Figure 3.

BaDV-2 IGR supports internal ribosome entry. (A) Bicistronic reporter RNAs containing either the CrPV IGR or the BaDV-2 IGR, (B) bicistronic RNAs with a 5′UTR strong hairpin, or (C) monocistronic reporter RNAs with a 5′UTR hairpin and the BaDV-2 IGR (top schematics) were incubated in Sf-21 extracts. In vitro transcribed RNAs were analyzed by agarose gel analysis (middle). Translation of FLuc and RLuc was measured by either radioisotope [³⁵S]-methionine/cysteine incorporation followed by SDS-PAGE and phosphorimager analysis or luciferase assays (graphs shown bottom). A paired t-test was used to determine the p values. ** p < 0.01. “n.s.” denotes the difference is not significant between the control groups and the experimental groups (p > 0.05). Shown are representative SDS-PAGE gels and the averages from at least three independent experiments ± standard deviation.