Figure 8.

Stop codon mutations to confirm the BaDV-2 IGR IRES initiation site. (A) Schematic of stop codon mutations along the BaDV-2 IGR IRES (upper). The red letters denote the predicted AUG start codon. In vitro translation assays were performed in Sf-21 cell lysate with the indicated mutations using bicistronic reporter RNAs. RLuc and FLuc activities were measured and normalized to the wild-type BaDV-2 bicistronic RNA (bottom). An ANOVA statistical test was used to determine the p value and thus the significance levels. Groups were compared to WT BaDV. ** represents p < 0.01. “n.s.” denotes the difference is not significant between the experimental groups and WT (p > 0.05). Shown are the averages from at least three independent experiments ± standard deviation.