TABLE 1.
Strains, plasmids, and phages used in this study
| Strain, phage, or plasmid | Genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| ECL116 | F− ΔlacU169 endA hsdR thi | 1 |
| SME1074 | ECL116 λ Φ(rhaS-lacZ)Δ216 recA::cat | 9 |
| SME1076 | ECL116 λ Φ(rhaS-lacZ)Δ216 Δ(rhaSR)::Km recA::cat | 9 |
| SME1461 | ECL116 λ Φ(galP1Δ4-lacZ) | 14 |
| SME1834 | ECL116 λ Φ(galP1Δ4-lacZ)/pSE207/pREP4 | 14; this study |
| SME1849 | ECL116 λ Φ(rhaB-lacZ)Δ226 | 14 |
| SME1850 | ECL116 λ Φ(rhaB-lacZ)Δ110 | 14 |
| SME1851 | ECL116 λ Φ(rhaB-lacZ)Δ84 | 14 |
| SME1853 | ECL116 λ Φ(rhaB-lacZ)Δ226 Δcrp zhc-511::Tn10 | 14 |
| SME1854 | ECL116 λ Φ(rhaB-lacZ)Δ110 Δcrp zhc-511::Tn10 | 14 |
| SME1855 | ECL116 λ Φ(rhaB-lacZ)Δ84 Δcrp zhc-511::Tn10 | 14 |
| SME2503 | ECL116 λ Φ(rhaS-lacZ)Δ128 recA::cat | This study |
| SME2504 | ECL116 λ Φ(rhaS-lacZ)Δ216 Δcrp zhc-511::Tn10 recA::cat | This study |
| SME2505 | ECL116 λ Φ(rhaS-lacZ)Δ128 Δcrp zhc-511::Tn10 recA::cat | This study |
| SME2508 | ECL116 λ Φ(rhaS-lacZ)Δ90 recA::cat | This study |
| SME2509 | ECL116 λ Φ(rhaS-lacZ)Δ90 Δcrp zhc-511::Tn10 recA::cat | This study |
| SME2521 | ECL116 λ Φ(rhaS-lacZ)Δ216 Δ(rhaSR)::Km Δcrp zhc-511::Tn10 recA::cat | This study |
| SME2523 | ECL116 λ Φ(rhaS-lacZ)Δ216 ΔSR::kan recA::cat | This study |
| SME2524 | ECL116 λ Φ(rhaS-lacZ)Δ128 ΔrhaS-Tn10 recA::cat | This study |
| SME2525 | ECL116 λ Φ(rhaS-lacZ)Δ128 Δ(rhaSR)::Km recA::cat | This study |
| SME2526 | ECL116 λ Φ(rhaS-lacZ)Δ128 Δ(rhaSR)::Km Δcrp-Tn10 recA::cat | This study |
| SME2529 | ECL116 λ Φ(rhaS-lacZ)Δ90 Δ(rhaSR)::Km recA::cat | This study |
| SME2530 | ECL116 λ Φ(rhaS-lacZ)Δ90 Δ(rhaSR)::Km Δcrp-Tn10 recA::cat | This study |
| Phages | ||
| λ RS45 | bla′-lacsc att+ int+ imm21 | 31 |
| λ SME106 | λRS45Φ(rhaS-lacZ)Δ216 | 9 |
| λ SME112 | λRS45Φ(rhaS-lacZ)Δ128 | This study |
| λ SME114 | λRS45Φ(rhaS-lacZ)Δ90 | This study |
| Plasmids | ||
| pGEM-11Zf(+) | Promega Gene Editor kit | |
| pREIIα | Apr pREIIα rpoA | 4 |
| pHTf1α | Apr pHTf1α rpoA | 33 |
| pJTC114 | AprrhaR+ in pTAC12 | 34 |
| pQE30 | Apr | QIAGEN QIAexpress kit |
| pREP4 | KanrlacIq | QIAGEN QIAexpress kit |
| pRS414 | Apr ′lacZ lacY+ lacA+ | 31 |
| pSE101 | Apr, rhaB+ rhaSR+ in pTZ18R | 9 |
| pSE104 | Apr pRS414 Φ(rhaB-lacZ)Δ110 | 9 |
| pSE117 | Apr pSR414 Φ(rhaS-lacZ)Δ128 | This study |
| pSE186 | Apr pHG165crp | 14 |
| pSE187 | Apr pSE186 (Thr158Ala) | 14 |
| pSE188 | Apr pSE186 (Gly162Ala) | 14 |
| pSE189 | Apr pSE186 (His19Ala) | 14 |
| pSE190 | Apr pSE186 (His21Ala) | 14 |
| pSE191 | Apr pSE186 (Lys101Ala) | 14 |
| pSE192 | Apr pREIIα rpoA-Δ235 | 14 |
| pSE193 | Apr pRS414 Φ(rhaS-lacZ)Δ90 | This study |
| pSE207 | Apr pQE30 His6-crp | This study |
| pSE213 | Apr pGEM-11Zf(+) rhaSR-rhaBAD | This study |
| pSE214 | Apr pGEM-11Zf(+)rhaSR-rhaBAD CRP site2− | This study |
| pSE215 | Apr pGEM-11Zf(+)rhaSR-rhaBAD CRP site3− | This study |
| pSE216 | Apr pRS414 Φ(rhaS-lacZ)Δ309 CRP site2− | This study |
| pSE217 | Apr pRS414 Φ(rhaS-lacZ)Δ309 CRP site3− | This study |
| pSE218 | Apr pRS414 Φ(rhaS-lacZ)Δ309 | This study |