Sequential glucosylation determined by NMR in the biosynthesis of mulberroside D, a cis-oxyresveratrol diglucoside, in Morus alba L. cell cultures

Abstract.

Three new diglucosides, mulberrosides D, E, and F, were isolated from Morus alba cell cultures and their structures were established by spectroscopy. In the biosynthesis of mulberroside D, a cinnamoylpolyketide-derived cis-oxyresveratrol diglucoside in the cell cultures, the different ratio of ¹³C-enrichment between two glucose moieties of mulberroside D, revealed by administering [U-¹³C₆]-D-glucose and [1,3-¹³C₂]glycerol to the cell cultures, suggested that two glucosylation steps in the biosynthesis of the glucoside occur with different time courses.