Abstract
Host immune responses are tightly controlled by various immune factors during infection, and protozoan parasites also manipulate the immune system to evade surveillance, leading to an evolutionary arms race in host‒pathogen interactions; however, the underlying mechanisms are not fully understood. We observed that the level of superoxide dismutase 3 (SOD3) was significantly elevated in both Plasmodium falciparum malaria patients and mice infected with four parasite species. SOD3-deficient mice had a substantially longer survival time and lower parasitemia than control mice after infection, whereas SOD3-overexpressing mice were much more vulnerable to parasite infection. We revealed that SOD3, secreted from activated neutrophils, bound to T cells, suppressed the interleukin-2 expression and concomitant interferon-gamma responses crucial for parasite clearance. Overall, our findings expose active fronts in the arms race between the parasites and host immune system and provide insights into the roles of SOD3 in shaping host innate immune responses to parasite infection.
Subject terms: Parasite immune evasion, Parasite host response, Malaria
Superoxide dismutase 3 is elevated in patients and animals infected by protozoan parasites such as Plasmodium. Here, Li et al. show that SOD3 expression is associated with experimental cerebral malaria and inhibition of host immune responses.
Introduction
Host immune responses are tightly controlled by various immune factors during infection1,2. Pathogens are known to hijack host factors3 or express an array of virulence factors4–7 that aim to overcome host immune defenses to achieve successful proliferation and dissemination. Host genetic and immune factors (HIFs) are the two major types of factors that are exploited by pathogens during infection8,9. The reactive oxygen species (ROS) are one types of the innate responses upon pathogen invasion10. However, ROS is not only detrimental to the invaded pathogens, but also harmful to the host cells. The host cells rely on the superoxide dismutases (SODs, including SOD1, SOD2 and SOD3) to scavenge the extra amount of ROS and the maintenance of the homeostasis. It is known that Plasmodia increased a substantial amount of SOD activity from host in the infection11. However, a fundamental challenge in studying the HIF-pathogen interactions is to understand the host derive-redox signaling that facilitates pathogen evasion of host immune responses. Superoxide dismutase 3 (SOD3) is the only SOD-like enzyme secreted from the host cells to the extracellular space that scavenges substantial amounts of ROS to protect the host from oxidative stress during infection12,13. Additionally, SOD1, primarily functions in the cytoplasm, has been reported to serve as an indicator of disease severity in individuals with various clinical manifestations of vivax malaria14. SOD2, mainly localized in the mitochondrial lumen, has been found to be notably upregulated in pre-asymptomatic malaria patients from Cameroon15. Although the roles of SOD1 and SOD2 in malaria are well characterized14,15, almost nothing is known about the contribution of SOD3 to host immune responses beyond ROS scavenging.
Here, we demonstrated that SOD3 mainly secreted by neutrophils could directly bind T cells and suppress its IL-2 production, and consequently reduced the recruitment of IFN-γ producing T cells in the responses to invading parasites. Our results revealed an essential role of SOD3 in responses to parasite invasion and targeting SOD3 may be an alternative way to enhance host protective responses and reduce disease severity in acute infection.
Results
The expression of SOD3 was elevated in malaria patients and protozoa infected mice
We compared the expression of SOD3 in mice infected with lethal Plasmodium y. yoelii YM at different time points post-infection. Contrast to that in uninfected mice, the expression of SOD3 in the splenocytes was significantly elevated when parasites were detectable in the blood (Fig. 1a and Supplementary Fig. S1a, b). Upregulation of serum SOD3 in WT mice after infection with P. berghei ANKA (another rodent malaria parasite that is also lethal to the host, Fig. 1b) and P. y. yoelii YM (Supplementary Fig. S1c) was also observed, and that elevation of serum SOD3 was associated with increased parasitemia. We further quantitatively analyzed SOD3 in the sera of patients infected with P. falciparum vs. that of healthy donors. The patients with a first malaria episode had significantly higher levels of SOD3 than either individual with chronic malaria or healthy donors from nonmalaria epidemic areas (Fig. 1c).
To determine whether elevated SOD3 expression is a general phenomenon associated with protozoan infection. We extended the study by measuring the SOD3 levels in mice infected with either Trypanosoma brucei brucei Lister 427 or Toxoplasma gondii RH, and it turned out to be the same as that observed in mice infected by the two Plasmodium species (Supplementary Fig. S1d, e). Therefore, we hypothesize that SOD3 is essentially associated with host vulnerability to protozoan infection.
To test this hypothesis, we used SOD3 knockout (SOD3−/−) mice, in which SOD3 was not detectable in the sera by LC‒MS/MS analysis (Supplementary Fig. S1f, g). We further compared the vulnerability of the SOD3−/− mice and their WT littermates to infection of lethal Plasmodium species. To our surprise, the SOD3−/− mice showed significant resistance, with substantially extended survival time, to the infection of either P. y. yoelii YM strain or P. y. yoelii 17XL strain, which are both lethal to mice (Fig. 1d, e). To further determine whether SOD3 is generally associated with host vulnerability to protozoan infection, SOD3−/− and WT mice were respectively infected with two other protozoan parasites, including Trypanosoma brucei brucei Lister 427 and Toxoplasma gondii (RH strain). SOD3−/− mice also showed significant resistance to the two parasite species compared to the WT mice (Fig. 1f, g). Therefore, these results suggested that SOD3 is essentially associated with host vulnerability to protozoa infection.
The expression of SOD3 is associated with experimental cerebral malaria (ECM)
Cerebral malaria is a severe complication which is caused by the sequestration on P. falciparum-infected erythrocytes in the cerebral microvasculature16. Cerebral pathogenesis of P. falciparum malaria was experimentally modeled with P. berghei ANKA-infected C57BL/6 mice17. Here, we found that the expression of SOD3 was higher in the brains of P. berghei ANKA-infected mice than that in the uninfected WT mice as illustrated in the immunohistochemistry analysis (Supplementary Fig. S2a). We next investigated whether SOD3 expression is associated with P. berghei ANKA-induced ECM. We used a transgenic luciferase-expressing line, P. berghei ANKA (PbA luc), to infect SOD3−/−, SOD3-overexpressing (SOD3o/e, isogenic to C57BL/6) and WT mice, and quantified the luminescence via in vivo luminescence imaging. More severe ECM were observed in the SOD3o/e mice (Fig. 2a). The sequestration of infected red blood cells (iRBCs) in the brain of SOD3o/e mice was approximately 1.5-fold more compared to that of WT mice (Fig. 2b). In contrast, the amount of sequestered iRBCs in the brain of SOD3−/− mice was significantly lower than that in WT mice (Fig. 2a, b), and the SOD3−/− mice survived substantially longed after P. berghei ANKA infection (Fig. 2c), while both SOD3o/e and WT mice succumbed to death due to ECM at the same time after infection (Fig. 2c, d). Further, the malaria-associated pathologies in the SOD3−/− mice was alleviated compared to WT mice (Supplementary Fig. S2b, c). Importantly, intravenously administration of recombinant SOD3 in SOD3−/− mice reversed their resistance to P. berghei ANKA infection (Fig. 2e). Furthermore, P. berghei ANKA isolated from SOD3−/− mice and WT mice displayed similar infectivity in naïve mice, suggesting that SOD3 had no direct effect on the parasites (Supplementary Fig. S3d and e). All data indicate that SOD3 is an important factor associated with host vulnerability to protozoa infection.
SOD3 suppresses the function of T cells in parasite infection
To investigate whether SOD3 can directly bind and regulate immune cells, we conducted cell binding experiments using the recombinant SOD3 protein (rSOD3). Result showed that the rSOD3 directly bound to T cells and NK cells (Fig. 3a). Early burst of IFN-γ production is associated with protection to P. berghei ANKA infection. Intriguingly, a significant increase of IFN-γ in early stage was detected in the SOD3−/−mice, indicating a negative correlation between SOD3 and IFN-γ production, which was even more significant during early infection (Fig. 3b). The results of immunohistochemical staining of spleen tissue using IFN-γ antibody confirmed the aforementioned findings (Fig. 3c). It is well known that CD8+T cells and NK cells are major sources of IFN-γ, which play a pivotal role in the control of invading pathogens18. Here, the IFN-γ-expressing CD8+T cell subsets were also expanded following P. berghei (Fig. 3d) or P. y. yoelii (Fig. 3e) infection in SOD3−/− mice, suggesting that SOD3 may inhibit the activity of T cells and depletion of SOD3 would result in expansion of these IFN-γ-expressing T, NK and NKT cell subsets (Supplementary Fig. S3a–e). As all three major types of IFN-γ-producing T, NK and NKT subpopulations express IFN-γ at steady state (Supplementary Fig. S3f–j), which indicated that SOD3 may be an add-on factor in modulation of IFN-γ expression. Moreover, IFN-γ depletion led to a significant reduction in survival time of 80% SOD3−/− mice after infection, confirming that IFN-γ plays a protective role in SOD3−/− mice (Fig. 3f).
To investigate whether SOD3 can directly inhibit the expansion of IFN-γ-producing T cell subpopulations, we performed T-cell activation and differentiation assay in vitro. rSOD3 could directly suppress T cell activation (Fig. 3g) and inhibited naive T cell differentiation into effector T cells (Fig. 3h, i). Importantly, rSOD3 significantly reduced the proportion of IFN-γ-producing T cells in vitro, suggesting that SOD3 directly inhibited the expansion of IFN-γ-producing T cells (Fig. 3j).
To further elucidate the molecular mechanism of T cell inhibition by SOD3, we prescreened and compared the expression of the sixteen proteins that regulate IFN-γ signaling in splenic cells in SOD3−/− and WT mice (Supplementary Fig. S4a). Elevated expression of nuclear factor kappa B (NF-κB), the signal transducer and activator of transcription 5 A (STAT5a) and c-Jun N-terminal Kinase (JNK) was identified in spleen from SOD3−/− mice compare to WT mice after infection, whereas reduction of NAD-dependent deacetylase sirtuin-1 (SIRT1) and mitogen-activated protein (MAP) kinases/P38 expression was observed in SOD3−/− mice compare to WT mice. The expression of five toll-like receptors, STAT5b and protein kinase B (PKB, or Akt) was relatively stable in the two experimental groups (Supplementary Fig. S5a). Importantly, an increased expression of JNK was observed in purified T cells in SOD3−/− mice after infection (Fig. 3k, l), and JNK has been known to control activation of NF-kb and STAT5a, we thus focused on its involvement in the regulation of IFN-γ expressing T cells. Inhibition JNK activity with a specific inhibitor SP600125 impaired proliferation of IFN-γ-producing T cells (Fig. 3m). Taking together, SOD3 was found to inhibit the expression of JNK in T cells and JNK activation is crucial for IFN-γ-mediated T-cell toxicity.
SOD3 suppressed IL-2 expression by T cells
IL-2 is a major T cell growth factor which promotes the expansion of IFN-γ expressing T cells19. Here, we observed a time-dependent increase in the serum IL-2 in SOD3−/− mice after parasite infection, but not IL-10, TNF-α, compared to that of WT mice (Fig. 4a, Supplementary Fig. S5a, b). Further, higher percentages of IL-2+ CD4+ T cells and IL-2+ CD8+ T cells in SOD3−/− mice than in WT mice persisted until late infection (Fig. 4b–e). Moreover, an increased proportion of IL-2-producing CD4+ T cells and CD8+ T cells was also observed in SOD3−/− mice after P. y. yoelii infection (Fig. 4f–h). Importantly, IL-2 depletion by infusion of IL-2-specific antibodies in vivo significantly increases susceptibility to Plasmodium infection (Fig. 4i, j). And intravenous injection of recombinant IL-2 rescued resistance to infection by significantly reduced parasitemia in WT mice (Fig. 4k), confirming that IL-2 plays a protective role in resistance to Plasmodium infection in SOD3−/− mice.
As SOD3 binds CD4+ T cells and CD8+ T cells (Fig. 3a), we therefore tested whether SOD3 inhibits T cells to generate IL-2. Addition of SOD3 into the splenic lymphocytes significantly reduced the proportion of IL-2-producing CD4+ T cells and CD8+ T cells, suggesting that SOD3 can directly regulate the expansion of IL-2-expressing T cells (Fig. 5a, b). To investigate whether the inhibition of IL-2-expressing T cells by SOD3 is causally related to JNK activation, we assessed the proportion of IL-2-producing T cells under JNK-deficient conditions. Inhibition of JNK activity led to the reduction of IL-2-producing CD4+ and CD8+ T cells (Fig. 5c, d). These data indicate that SOD3 can directly suppress the expansion of IL-2-producing CD4+ and CD8+ T cells, which may require JNK activation. SOD3 deficiency significantly increased the proportion of IFN-γ expressing CD122+ (IL-2 receptor) T cells without altering the proportion of CD122+ T cells (Fig. 5e, f). Furthermore, neutralization with IL-2-specific antibody resulted in a striking reduction of IFN-γ levels in the sera (Fig. 5g). Overall, our data suggest that SOD3 suppresses T cells by inhibition of IL-2 autocrine pathways, thereby benefits parasites in evading host immune surveillance.
Neutrophils are the main cellular sources of SOD3
To identify the source of splenic SOD3, we analyzed available single-cell transcriptome data (Mouse Cell Atlas, https://bis.zju.edu.cn/MCA/) from healthy mice and the data indicated that SOD3 was primarily expressed in splenic macrophages and neutrophils. Correspondingly, we found, in WT mice, the proportions of CD11bhi macrophages (Fig. 6a) and neutrophils (Fig. 6b) after infection doubled compared to uninfected controls, while other subpopulations decreased (Fig. 6c, d). We thus sorted splenic macrophages and neutrophils at different time points post-infection using fluorescence-activated cell sorting and examined the expression of SOD3 in these cells. The results of RT–qPCR, immunofluorescence and Western blotting illustrated that only neutrophils showed a significant increase in SOD3 mRNA transcription (Fig. 6e, f) and protein expression after infection (Fig. 6g, h). In agreement with the above results, the adoptive transfer of neutrophils, but not macrophages, from WT mice into SOD3−/− mice significantly reduced the survival time of SOD3−/− mice after parasite infection (Fig. 6i, j). These data strongly suggest that the parasites evaded immune surveillance by inducing SOD3 secretion from neutrophils, which suppress IL-2 and IFN-γ-mediated host immune responses to protozoa infection.
Discussion
Pathogen infection triggers oxidative stress by generating an imbalance between the oxidant and antioxidant systems. SOD3, a member of the superoxide dismutase family, has been primarily recognized as an enzyme for scavenging ROS in extracellular spaces. In this study, we revealed a function of SOD3, which was upregulated during protozoan infection. However, the overexpression of SOD3 was surprisingly found to dysregulate the host innate immune response to invading parasites. SOD3, primarily expressed by neutrophils, can directly bind and suppress the expansion of IL-2-expressing T cells, concomitantly reducing the expansion of IFN-γ-expressing T cells, possibly by inhibiting the JNK pathway. This eventually results in significantly weakened host immune resistance to parasite infection (Figs. 1–6, Supplementary Fig. S6). Overall, our results revealed an unexpected link between invading parasites and host neutrophils, highlighting that SOD3 could be a target for mitigation of inflammation and hypertension in the context of infectious diseases.
An essential pathophysiological role of SOD3 has been noticed in inflammatory diseases and autoimmune disorders20–22. However, there have been no systemic investigations on the relevance between SOD3 and parasite infection. Here, we systematically scrutinized the involvement of SOD3 in the suppression of early innate immune reaction after parasite infection and deciphered the underlying mechanism. The data suggest that SOD3 acted as an immune dysregulatory factor in the infections, which is supported by the fact that SOD3−/− mice were significantly more resistant than their WT littermates to infection with either lethal or nonlethal parasite species (Figs. 1 and 2). IL-2 derived from CD4+ T cell seemed to play a critical role in the SOD3−/− mice, as neutralization of IL-2 with specific antibodies significantly reduced the survival time of the mice compared with the controls after parasite infection. Furthermore, the fact that the preferential binding of SOD3 to T cells and inhibition of the expansion of IL-2 expressing or IFN-γ-producing T cells and downstream IFN-γ production also strongly indicated SOD3 played an important regulation in immune responses to protozoa infection.
Studies have strongly suggested that an early burst of IFN-γ production is associated with protection to P. falciparum and P. berghei ANKA infection23,24. Parasite-related HIFs have been reported to facilitate immune evasion by inhibiting IFN-γ-mediated pathogen clearance3,25–28. Here, we showed that IFN-γ responses in SOD3−/− mice were augmented and neutralization of IFN-γ in SOD3−/− mice at early infection resulted in reduced survival time in 80% of SOD3−/− mice. Moreover, neutralization of IL-2 at early time also led to decreased IFN-γ production in SOD3−/− mice in response to parasite infection. Thus, the protective effect of SOD3 deficiency is mediated by early production of IFN-γ. The observations in this study demonstrate important differences in baseline immunity between SOD3 null mice and their WT control, suggesting that SOD3 efficiently targets IFN-γ and IL-2 producing T cells. These data support the conclusion that SOD3 is a pivotal immune factor in modulation of host innate immune responses to parasite infection by controlling the production of IL-2 and IFN-γ.
While we believe that these results presented in this study is an advance in understanding the interaction between the invade protozoan parasites and host immune system, the exact mechanism underlies the possible regulation of SOD3 on the T cells remains further investigation. The reason is that, even though the active SOD3 protein showed direct inhibition on T cell function in both in vitro and in vivo conditions, other functions of SOD3 may mediate the effects independent of its enzymatic activity and affinity to the immune cells. Further, a significant increase in JNK expression was indeed observed in T cells sorted from SOD3−/− mice after infection, there may be an unknown link between SOD3 and the kinase activity of the JNK protein.
Overall, this study expands our understandings of how neutrophil-derived SOD3 can modulate immune responses and argue for the importance in keeping the balance between the antioxidant activities and the potent immune responses to invading pathogens.
Methods
Ethics
The animal experiments were conducted according to the animal husbandry guidelines of Shenyang Agricultural University (permit no. SYXK<Liao>2021-0010). Human sera samples were obtained under approved protocols of the Ethical Committee of the Chinese Academy of Medical Sciences (approval no. IPB-2016-2) and Institutional Review Board (no. PRAMS0034319) of the Pennsylvania State University.
Animals
Female and male C57BL/6 mice were obtained from Liaoning Changsheng Biological Technology Company (Liaoning, China). C57BL/6-Sod3tm1cyagen (SOD3−/−) mouse strains (Serial Number: KOCMP-22050-Sod3) were purchased from Cyagen Biosciences (Suzhou, China). Mice were maintained under the following housing conditions: ambient temperature 22 °C, humidity control 50%, 12 h light/12 h dark cycle. Littermates used in the tests were of the same sex and similar body weights as the SOD3−/− mice. SOD3 transgenic mice (Global SOD3 overexpression) were constructed with the help from GRENSTER Co., Ltd. (Liaoning, China) and achieved by breeding a CAG-SOD3-IRES-ZsGreen1 mouse to transgenic mice, which globally overexpressed SOD3, with an average protein expression level ~1.4-fold higher than normal in serum. In this study, we have used humane endpoints for the infected animals. To determine when the animals should be euthanized, we used the specific signs such as weight loss, inability to rise or ambulate, or dehydration. In Kaplan-Meier survival analysis, mice that are euthanized in humane endpoints are considered “censored” data to distinguish them from mice where death actually occurred.
Sera from P. falciparum malaria patients and healthy donors
All procedures performed on human samples were carried out in accordance with the tenets of the World Medical Association’s Declaration of Helsinki. A total of 20 patients (ten male and ten female) suffering from falciparum malaria were recruited from a malaria-endemic area, all patients provided with written informed consent as previously described28. Another serum samples were collected from P. falciparum–infected patients (24 male)29 with a recent history of travel to malaria-endemic African countries, and P. falciparum infection was clinically confirmed by a microscopy. The inclusion criteria were no history of P. falciparum infection. Healthy control sera were collected from healthy volunteers with informed consent. The information of all individuals involved was anonymized. Written consent for the publication of this study was obtained from all individuals.
Experimental malaria models
Plasmodium species lethal to rodents, including P. y. yoelii YM, P. y. yoelii 17XL and P. berghei ANKA, were donated by Dr. Yaming Cao and propagated by passage in C57BL/6 male mice of 7–8 weeks old; a transgenic P. berghei ANKA parasite strain expressing luciferase under the control of the ef1a promoter (PbA luc)28 was provided by Dr. Wenyue Xu (Army Medical University, China) and propagated by passage in mice.
Preliminary ECM experiments in mice were performed to optimise the infection dose. Of the four doses tested (102, 103, 104, 105 iRBCs), the minimum infection dose of 103 P. berghei ANKA parasites was collected and injected intraperitoneally into 7–8-week-old male C57BL/6 naïve mice to initiate ECM experiments, minimizing pain and distress to the mice. Humane endpoints were defined as serious neurological signs or immobility. Parasitemia was determined by examination of Giemsa-stained thin blood smears by light microscopy every other day after infection.
Detection of the accumulation of malaria parasites in the mouse brains by in vivo imaging
Upon P. berghei–luc infection, the accumulation of parasites in the brain and in the body was assessed in SOD3o/e mice as well as SOD3−/− mice and WT mice with an AniView600 multimode in vivo animal imaging system (Guangzhou Biolight Biotechnology Co., Ltd., China) after intraperitoneal injection of D-Luciferin (High Purity, Biyuntian, China, Cat# ST198). At least three mice were used in each group.
T. brucei cultivation and infection
The T. brucei Lister 427 strain30 was donated by Dr. Zhaorong Lun (Sun Yat-sen University, China) and maintained in our laboratory. The bloodstream form of T. brucei was proliferated in Hirumi’s modified Iscove’s-9 medium and propagated by passage in mice. SOD3−/− mice and WT mice were inoculated intraperitoneally with 50 T. brucei parasites. Parasitemia was determined by evaluating tail blood by light microscopy with a hemocytometer each day after infection.
T. gondii RH cultivation and infection
The T. gondii RH strain was maintained in our laboratory. The parasites were expanded in Vero cells and propagated by passage in mice. T. gondii RH strain tachyzoites were grown under the indicated conditions and collected by filtration as previously reported31. SOD3−/− mice and WT mice were inoculated intraperitoneally with 1 × 104 tachyzoites.
In vivo IFN-γ and IL-2 depletion
For in vivo depletion of IFN-γ and IL-2, SOD3−/− mice were injected through the tail vein with anti–IFN-γ monoclonal antibody (mAb) (Clone R4-6A2, Selleck Chemicals, USA) and anti–IL-2 mAb (Clone JES6-1A12, Leinco Technologies, St. Louis, MO, every other day). SOD3−/− mice in the control group were injected intraperitoneally with the corresponding isotype control IgG. Parasitemia was determined by examination of Giemsa-stained thin blood smears by light microscopy every other day after infection. The parasitemia was quitted after the total death of the animals in the control group.
SOD3 rescue experiment
For rescue experiments, SOD3−/− mice were injected with recombinant mouse active SOD3 protein every other day from day 0–10 after P. berghei infection (Cat# APA117Mu01, Cloud clone crop, China, purity> 95%). The injected concentrations of SOD3 corresponded to that of SOD3 in WT mice after infection. SOD3 has a long half-life of ~ 20 h, and the endotoxin level was determined by the limulus amoebocyte lysate method. The specific activity of recombinant mouse SOD3 is 273.8 U/mg. SOD3−/− mice in the control group were administered with the same volume of saline buffer via tail vein injection.
Adoptive transfer of neutrophils and macrophages from WT mice to SOD3−/− mice
Splenic neutrophils and macrophages were positively selected as before32. The purity of sorted cells, as verified by FACS analysis, was more than 95%, which separated intact live cells from dead cells and enucleated cellular debris. The proportion of dead cells was determined by using a hemocytometer to count the cells stained with trypan blue and was lower than 5%. Fifteen SOD3−/− mice were randomly allocated to three groups with five animals in each group. In the SOD3−/− + WT macrophage group, SOD3−/− mice (recipients) received 1.5 × 106 macrophages from WT mice i.v. (SOD3+ macrophages→SOD3−/− host). In the SOD3−/− mice + WT neutrophils group, SOD3−/− mice (recipients) received only 1.5 × 106 neutrophils from WT mice i.v. (SOD3+ neutrophils→SOD3−/− host). SOD3−/− mice and WT mice in the control group received the same volume of saline buffer. Meanwhile, mice in all groups were inoculated intraperitoneally with iRBCs.
Immunofluorescence and immunohistochemical analysis of SOD3 expression in splenic and brain tissues of WT and SOD3−/− mice
For immunofluorescence analysis, mice were sacrificed at day seven after P. y. yoelii YM infection, and the spleens were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned. In brief, spleen sections were blocked with 10% FBS in PBS, incubated with anti-SOD3 antibodies (Affinity Biosciences, China, Cat# DF7753) and anti-myeloperoxidase primary antibodies (MPO, Abcam, UK, Cat# ab208670) in 5% FBS in PBS for 1 h at 37 °C, washed five times with PBS, and incubated with the corresponding secondary antibodies according to the IF staining procedure. The same procedure was performed to stain healthy spleen sections.
For immunohistochemical analysis, P. berghei ANKA-infected mice were sacrificed and the brain and spleen were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned. Brain sections were blocked with 10% FBS in PBS, incubated with anti-SOD3 antibodies (Affinity Biosciences, Cat# DF7753, RRID: AB2841219), incubated with a corresponding secondary antibody, and then stained with 3,3-diaminobenzidine. Spleen sections were incubated with a rabbit anti-IFN gamma antibody (Bioss, Beijing, China, Cat# bs-0480R). The same procedure was performed to stain healthy brain and spleen sections.
Detection of SOD3 in the sera of both parasite-infected mice and humans using enzyme-linked immunosorbent assay (ELISA)
The concentrations of SOD3 in the sera of WT mice at different time points post-infection were detected using a mouse SOD3 ELISA kit (CLOUD-CLONE CORP., Cat# SEA117Mu) according to the manufacturer’s instructions. Serum samples require about a 100-fold dilution. Three healthy mice were used as controls. Briefly, 100 µL of different concentrations of standards and samples were added to each well. The wells were then covered with an adhesive strip and incubated for 2 hours at 37 °C. After removing the liquid from each well, 100 µL of biotin antibody (1x) was added to each well after three washes with PBS. The wells were covered with a adhesive strip and incubated for 1 hour at 37 °C. Next, each well was aspirated and washed three times. After washing, 100 µL of HRP-avidin (1x) was added to each well and incubated for 1 hour at 37 °C, followed by five washes. Finally, after the addition of TMB substrate, the optical density (OD) at 450 nm was measured. The minimum detectable dose of SOD3 is typically less than 1.27 ng/mL.
The concentrations of SOD3 in the sera of healthy volunteers and P. falciparum–infected patients were detected using a Human SOD3 ELISA kit (Elabscience, Wuhan, China, Cat# E-EL-H2382) according to the manufacturer’s instructions. Same experimental process was followed as explained above.
LC‒MS/MS analysis of serum SOD3
Serum proteins were extracted from WT and SOD3−/− mice, and the high-abundance proteins in the sera were removed using the ProteoMiner protein enrichment kit (Bio-Rad, USA), followed by enzymatic digestion, enrichment of informative peptides, and LC‒MS/MS (Orbitrap Exploris 480) for separation and detection. The search parameters were set as follows: mass tolerance for precursor ion was 10 ppm, and mass tolerance for product ion was 0.02 Da. SOD3 protein levels were calculated using Proteome Discoverer 2.4 (Thermo). The identified SOD3 protein contains at least 1 unique peptide.
Statistics and reproducibility
Student’s t-test was used to calculate significance between two groups and ANOVA test was used to calculate differences for more than two groups. All data are shown as mean values and errors bars represent standard deviation. Each replicate was biologically independent. The sample size and number of replicates were determined based on previous studies.
Pearson correlation and regression analyses were used for the correlation studies. Correlation analysis was performed using the function “cor” of the base R package by applying Pearson’s method, using default parameters unless otherwise specified. R (version 4.0.3) and Graph Pad Prism (version 9) was used for all statistical analyses. Uncropped blots are found in Supplementary Fig. 7. The gating strategy is given in Supplementary Fig. 8.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Supplementary information
Source data
Acknowledgements
This research was supported by a grant from the National Key Research and Development Program of China (grant number 2022YFD1800200) to Q.C., the National Nature and Science Foundation of China (grant number 82030060) to Q.C., the CAMS Innovation Fund for Medical Sciences (CIFMS) (grant number 2019-I2M-5-042) to N.J., and the NIH grant (U19 AI089672) to L.C. Figures 1a, 6h, i, Figures S2d, and S6 Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
Author contributions
Q.L. performed most experiments, analyzed the data, and wrote the first draft of the manuscript. N.J. supervised the study. K.L. performed all Western blot and assisted with animal experiments, T.L. performed in vitro experiments using primary T cell, Y.Y., L.Y., Z.S., Y.Z., and A.F. assisted with the animal experiments. N.H., L.C., and Y.C. were responsible for collection of serum and confirmation of infection. W.X provided the luc-expression P. berghei parasite strain. L.Y., Z.S., N.H., and A.F. performed the infection experiment. X.S., Y.F., and R.C. assisted with the flow cytometry experiments. Q.C. conceived the study, analyzed the data, and finalized the manuscript.
Peer review
Peer review information
Nature Communications thanks Katsuyuki Yui and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. A peer review file is available.
Data availability
All data supporting the findings of this study are available within the paper. Source data are provided with this paper.
Competing interests
The authors declare no competing interests.
Footnotes
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
These authors contributed equally: Qilong Li, Kunying Lv, Ning Jiang.
Supplementary information
The online version contains supplementary material available at 10.1038/s41467-024-49348-0.
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Data Availability Statement
All data supporting the findings of this study are available within the paper. Source data are provided with this paper.