Extended Data Fig. 11 |. Microbiome engineering to enhance fatty acid amide production and exercise performance.

a, OEA levels in GF mice and GF mice mono-colonized with Coprococcus eutactus. b, Schematic of generation of Escherichia coli expressing the ereA-T gene cluster from Eubacterium rectale. c, OEA levels in GF mice and GF mice mono-colonized with either E. coli^ereA−T or the empty vector-containing strain E. coli^WT. d, e, Averaged recording traces (d) and quantification (e) of calcium imaging of DRG neurons exposed to in stool extracts from GF mice and GF mice mono-colonized with either E. coli^ereA−T or E. coli^WT. f, g, Averaged hourly distance (f) and quantification (g) of voluntary wheel activity of GF mice and GF mice mono-colonized with either E. coli^ereA−T or E. coli^WT. Inset shows representative recording traces. h-k, Kaplan-Meier plots (h, j) and quantifications (i, k) of distance (h, i) and time ( j, k) on treadmills by GF mice and GF mice mono-colonized with either E. coli^ereA−T or E. coli^WT. l, m, UMAP plot of all cell types identified in DRGs²⁰ (l) and expression of Trpv1 and Cnr1 (m). n-p, Expression of Arc (n) and Fos (o) in the dorsal root ganglia, and dopamine levels in the striatum (p) of exercised Cnr1-deficient mice and controls. q, r, Averaged hourly distance (q) and quantification (r) of voluntary wheel activity of Cnr1-deficient mice and controls. Inset shows representative recording traces. s, t, Kaplan-Meier plot (s) and quantification (t) of distance on treadmills by Cnr1-deficient mice and controls. Error bars indicate means ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001. Exact n and p-values are presented in Supplementary Table 2.