a, Schematics representing AAV spread (shaded regions) and fiber placements (rectangles) for every animal related to experiments in Fig. 5j-n and Extended Data Fig. 2k, l. Each animal is represented by a different colour. See Fig. 5k for a representative histological image. b, Schematics representing AAV spread (shaded regions) for every animal related to experiments in Extended Data Fig. 1g-j. Each animal is represented by a different colour. c, Representative image of hM4Di-mCherry expression in PVHMc4r neuron somas. d, Schematics representing AAV spread (shaded regions) for every animal related to experiments in Extended Data Fig. 1k-m. Each animal is represented by a different colour. e, Representative image of hM3Dq-mCherry expression in POMC neuron somas in the arcuate nucleus. Scale bar = 200 μm. 3V = Third ventricle. The schematics were created using The Mouse Brain in Stereotaxic Coordinates Second Edition (Paxinos and Franklin).