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. 2024 Jun 12;19(6):e0305647. doi: 10.1371/journal.pone.0305647

Correction: Distribution difference of colostrum-derived B and T cells subsets in gilts and sows

The PLOS ONE Staff
PMCID: PMC11168617  PMID: 38865369

There is an error in Table 1. For lines 7, 8, 11, 12, 13, 16, 18, 19, 20, 21, 22, 23 and 24, the value in the column “Working Dilution” is not “10-Jan.” The correct value “1/10.” The publisher apologizes for the error.

Table 1. Antibodies used in Flow Cytometry.

Antibody Species Clone Fluorochrome Labeling strategy Isotype Concentration (mg/ml) Working dilution
7-AAD NA NA 7-AAD Dye NA 0.1 1/400
granulocyte mouse anti-pig 6D10 FITC Directly conjugated IgG2a 0.1 Neat
CD45RA mouse anti-pig MIL13 FITC Directly conjugated IgG1 0.1 Neat
CD4alpha mouse anti-pig MIL17 FITC Directly conjugated IgG2b 0.1 Neat
IgM goat anti-pig AAI48 FITC Directly conjugated IgM 1.0 1/100
CD5 mouse anti-pig 1H6/8 FITC Directly conjugated IgG2a 0.1 Neat
CD14 mouse anti-pig MIL2 FITC Directly conjugated IgG2b 0.1 1/10
CD79a Mouse anti-human HM57 RPE Directly conjugated IgG1 Not determined 1/10
CD4alpha mouse anti-pig MIL17 RPE Directly conjugated IgG2b Not determined 1/100
CD8alpha mouse anti-pig MIL12 RPE Directly conjugated IgG2a Not determined 1/100
CD45RA mouse anti-pig MIL13 RPE Directly conjugated IgG1 Not determined 1/10
CD16 mouse anti-pig G7 RPE Directly conjugated IgG1 Not determined 1/10
CD3 mouse anti-pig PPT3 RPE-Cy7a Secondary antibodya IgG1 0.1 1/10
SWC7 or WC4 mouse anti-bovine CC55 RPE-Cy7a Secondary antibodya IgG1 0.1 Neat
macrophages mouse anti-pig BA4D5 APCb Secondary antibodyb IgG2b 0.1 Neat
SWC2 or CD27 mouse anti-pig B30C7 APC Directly conjugated IgG1 Not determined 1/10
CD335 mouse anti-pig VIV-KM1 APC Directly conjugated IgG1 Not determined 1/100
IgG1 isotype control mouse NA FITC Directly conjugated IgG1 0.1 1/10
IgG2a isotype control mouse NA FITC Directly conjugated IgG2a 0.1 1/10
IgG2b isotype control mouse NA FITC Directly conjugated IgG2b 0.1 1/10
IgG1 isotype control mouse NA RPE Directly conjugated IgG1 Not determined 1/10
IgG2a isotype control mouse NA RPE Directly conjugated IgG2a Not determined 1/10
IgG2b isotype control mouse NA RPE Directly conjugated IgG2b Not determined 1/10
IgG1 isotype control mouse NA APC Directly conjugated IgG1 Not determined 1/10
IgG1 isotype control mouse anti-pig NA RPE-CY7a Secondary antibodya IgG1 0.1 Neat

a MAb dilution following PE-Cy7 labeling (Serotec).

Reference

  • 1.Forner R, Bombassaro G, Bellaver FV, Maciag S, Fonseca FN, et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows. PLOS ONE 16(5): e0249366. 10.1371/journal.pone.0249366 [DOI] [PMC free article] [PubMed] [Google Scholar]

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