There is an error in Table 1. For lines 7, 8, 11, 12, 13, 16, 18, 19, 20, 21, 22, 23 and 24, the value in the column “Working Dilution” is not “10-Jan.” The correct value “1/10.” The publisher apologizes for the error.
Table 1. Antibodies used in Flow Cytometry.
Antibody | Species | Clone | Fluorochrome | Labeling strategy | Isotype | Concentration (mg/ml) | Working dilution |
---|---|---|---|---|---|---|---|
7-AAD | NA | NA | 7-AAD | Dye | NA | 0.1 | 1/400 |
granulocyte | mouse anti-pig | 6D10 | FITC | Directly conjugated | IgG2a | 0.1 | Neat |
CD45RA | mouse anti-pig | MIL13 | FITC | Directly conjugated | IgG1 | 0.1 | Neat |
CD4alpha | mouse anti-pig | MIL17 | FITC | Directly conjugated | IgG2b | 0.1 | Neat |
IgM | goat anti-pig | AAI48 | FITC | Directly conjugated | IgM | 1.0 | 1/100 |
CD5 | mouse anti-pig | 1H6/8 | FITC | Directly conjugated | IgG2a | 0.1 | Neat |
CD14 | mouse anti-pig | MIL2 | FITC | Directly conjugated | IgG2b | 0.1 | 1/10 |
CD79a | Mouse anti-human | HM57 | RPE | Directly conjugated | IgG1 | Not determined | 1/10 |
CD4alpha | mouse anti-pig | MIL17 | RPE | Directly conjugated | IgG2b | Not determined | 1/100 |
CD8alpha | mouse anti-pig | MIL12 | RPE | Directly conjugated | IgG2a | Not determined | 1/100 |
CD45RA | mouse anti-pig | MIL13 | RPE | Directly conjugated | IgG1 | Not determined | 1/10 |
CD16 | mouse anti-pig | G7 | RPE | Directly conjugated | IgG1 | Not determined | 1/10 |
CD3 | mouse anti-pig | PPT3 | RPE-Cy7a | Secondary antibodya | IgG1 | 0.1 | 1/10 |
SWC7 or WC4 | mouse anti-bovine | CC55 | RPE-Cy7a | Secondary antibodya | IgG1 | 0.1 | Neat |
macrophages | mouse anti-pig | BA4D5 | APCb | Secondary antibodyb | IgG2b | 0.1 | Neat |
SWC2 or CD27 | mouse anti-pig | B30C7 | APC | Directly conjugated | IgG1 | Not determined | 1/10 |
CD335 | mouse anti-pig | VIV-KM1 | APC | Directly conjugated | IgG1 | Not determined | 1/100 |
IgG1 isotype control | mouse | NA | FITC | Directly conjugated | IgG1 | 0.1 | 1/10 |
IgG2a isotype control | mouse | NA | FITC | Directly conjugated | IgG2a | 0.1 | 1/10 |
IgG2b isotype control | mouse | NA | FITC | Directly conjugated | IgG2b | 0.1 | 1/10 |
IgG1 isotype control | mouse | NA | RPE | Directly conjugated | IgG1 | Not determined | 1/10 |
IgG2a isotype control | mouse | NA | RPE | Directly conjugated | IgG2a | Not determined | 1/10 |
IgG2b isotype control | mouse | NA | RPE | Directly conjugated | IgG2b | Not determined | 1/10 |
IgG1 isotype control | mouse | NA | APC | Directly conjugated | IgG1 | Not determined | 1/10 |
IgG1 isotype control | mouse anti-pig | NA | RPE-CY7a | Secondary antibodya | IgG1 | 0.1 | Neat |
a MAb dilution following PE-Cy7 labeling (Serotec).
Reference
- 1.Forner R, Bombassaro G, Bellaver FV, Maciag S, Fonseca FN, et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows. PLOS ONE 16(5): e0249366. 10.1371/journal.pone.0249366 [DOI] [PMC free article] [PubMed] [Google Scholar]