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. Author manuscript; available in PMC: 2024 Jul 20.
Published in final edited form as: Cell. 2023 Jul 5;186(15):3166–3181.e18. doi: 10.1016/j.cell.2023.06.003

Figure 3. Dynamics of human blastocyst formation.

Figure 3.

(A) Live-imaging of a human blastocyst stained with SPY650-DNA and SPY555-actin. After 1–2 hours cell-cell junctions become clearly labeled by SPY555-actin.

(B) 2D planes of a blastocyst during cavitation. A cell division within the ICM can be tracked over time and computationally segmented. Imaging through the blastocyst also reveals tether-like structures projecting between the trophectoderm and ICM validated by Phalloidin-555 staining in fixed embryos.

(C) Selected frames of a live human embryo undergoing hatching.

The zona pellucida was masked out in live human embryos to improve embryo visualization. Scale bars, 10 μm.

See also Figure S4.