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. Author manuscript; available in PMC: 2024 Jun 17.
Published in final edited form as: Vaccine. 2023 Aug 22;41(44):6502–6513. doi: 10.1016/j.vaccine.2023.08.037

Fig. 8. Pseudovirus neutralization titers of AH-adsorbed DCFHP produced in Expi293 and CHO cells.

Fig. 8.

Mice were immunized with 10 mcg DCFHP, produced in either Expi-293 (transient expression) or CHO (stable expression) along with 150 mcg of alum (Alhydrogel). Some mice were boosted 21 days after the primary immunization as indicated in the figure. Blood samples were collected at indicated time points and the neutralization capacity of the serum samples were analyzed for SARS-CoV-2 wild type (A) and B.1.1.529.1 (Omicron) variant (B) using pseudovirus and HeLa cells co-expressing ACE2 and TMPRSS2. The neutralization titers are expressed as log10 values of 50 percent of neutralization titers (NT50). Note that the neutralization capacity is indistinguishable among the groups immunized with Expi-DCFHP and CHO-DCFHP. The average neutralization titers for each group are indicated below each bar. * LOQ, Lower limit of Quantitation.