Figure 6.
Effect of the antidepressants on HO-1 levels in Aβo-stimulated SH-SY5Y cells. (A,B) SH-SY5Y cells were treated with the three antidepressants + Aβo for 24 h after WAY-100635 pretreatment for 1 h. The p-value in ANOVA was 0.0150. Each value expresses the mean ± SEM of 6 individually treated samples (Tukey’s test). #, p < 0.05, versus Aβo + 0.1% DMSO-exposed cells. In the absence of 5 μM Aβo, HO-1 levels of Flx-, Dlx-, and Mir-treated cells were 3.27 ± 0.29, 3.61 ± 0.34, and 3.20 ± 0.48 fluorescence intensity × 104/μg protein (no significant difference, n = 6, Tukey’s test). Aβo, Aβ oligomer; DMSO, dimethyl sulfoxide; HO-1, heme oxygenase 1; Flx, fluoxetine; Dlx, duloxetine; Mir, mirtazapine; NS, not significant; SEM, standard error of the mean; ANOVA, analysis of variance.