Figure 5.

Necrosulfonamide (NSA) treatment reduces aortic leukocyte accumulation and angiogenesis in experimental abdominal aortic aneurysms. Frozen aortic sections from vehicle- and NSA (5 mg/kg body weight)-treated, angiotensin II-infused mice were fixed with acetone and stained with antibodies against CD68 for macrophages, CD4 for CD4⁺ T cells, CD8 for CD8⁺ T cells, CD31 for neovessels. (A) Representative immunohistochemical staining images for macrophages, CD4⁺ T cells, CD8⁺ T cells, and neovessels in two treatment groups. (B) Quantification (mean ± standard deviation) of macrophage accumulation. (C–E) Quantification (median and interquartile range) of CD4⁺ T cells (C), CD8⁺ T cells (D), and CD31⁺ neovessels (E) per aortic cross-section (ACS). Student’s t test (B) and non-parametric Mann–Whitney (C–E), * p < 0.05 compared to vehicle treatment. CD: Cluster of differentiation.