Figure 6.

Microinjection of miR-71-5p inhibitor: (a) Injection of the miR-71-5p inhibitor significantly reduced the expression level of miR-71-5p by 34.40% compared with the control group. (b) Inhibition of miR-71-5p resulted in an upregulation of Ae-GRD expression. (c) No significant change in mortality was observed in the miR-71-5p inhibitor group after 24 h of ivermectin treatment. Data represent three biological replicates, each consisting of three technical replicates. Statistical analysis was conducted using the t-test. “ns” indicates no significant difference, and asterisks denote levels of statistical significance: * p < 0.05, ** p < 0.01.