Skip to main content
Frontiers in Cellular and Infection Microbiology logoLink to Frontiers in Cellular and Infection Microbiology
. 2024 Jun 13;14:1419131. doi: 10.3389/fcimb.2024.1419131

Corrigendum: The effect of low-abundance OTU filtering methods on the reliability and variability of microbial composition assessed by 16S rRNA amplicon sequencing

Maria Nikodemova 1,2,, Elizabeth A Holzhausen 1,3,*,, Courtney L Deblois 4,5, Jodi H Barnet 1, Paul E Peppard 1, Garret Suen 4, Kristen M Malecki 1,6
PMCID: PMC11208917  PMID: 38938881

Error in Figure/Table

In the published article, there was an error in Table 2 as published. Table 1 was incorrectly duplicated, while Table 2 was omitted. The corrected Table 2 and its caption The effect of filtering method on difference α-diversity metrics appear below.

Table 2.

The effect of filtering method on different α-diversity metrics.

Filtering method Observed OTUs
Mean (SE)
Chao1
Mean (SE)
Shannon
Mean (SE)
Inverse Simpson
Mean (SE)
No filtering
 All OTUs included 266 (4.5) 380.9 (7.6) 3.243 (0.02) 13.289 (0.49)
Based on OTU abundance in whole dataset
 Filter OTUs with <0.1% abundance in dataset 65 (0.6)** 67.9 (0.7)** 2.922 (0.03)** 11.299 (0.38)*
 Filter OTUs without >10 reads in at least one sample in dataset 189 (2.1)** 219.0 (2.6)** 3.218 (0.03) 13.196 (0.49)
Based on OTU abundance in individual sample
 Filter OTUs with one read 188 (2.7)** 188 (2.7)** 3.2 (0.03) 13.2 (0.5)
 Filter OTUs with < 10 reads 106 (1.3)** 106 (1.3)** 3.164 (0.03)* 12.955 (0.48)
Based on OTU abundance in triplicate
 Filter OTUs without ≥10 reads in at least one of the three replicates 121 (1.4)** 121.4 (1.4)** 3.180 (0.03) 13.029 (0.48)
 Filter OTUs if not present in all three replicates 169 (2.2)** 182.2 (2.6)** 3.206 (0.03) 13.141 (0.49)

*p<0.05 vs All OTUs included, **p<0.001 vs All OTUs included.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.


Articles from Frontiers in Cellular and Infection Microbiology are provided here courtesy of Frontiers Media SA

RESOURCES