Table 1.
Gene | Forward primer sequence | Reverse primer sequence |
---|---|---|
ACTA2 | 5' -CTG TTG CAG CCA TCC TTC AT- 3' | 5' -TCA TGA TGC TGT TGT AGG TGG- 3' |
ADRP | 5' -TCA GCT CCA TTC TAC TGT TCA CC- 3' | 5' -CCT GAA TTT TCT GAT TGG CAC- 3' |
COL1A1 | 5' -ATG TTC AGC TTT GTGGAC CTC- 3' | 5' -CTG TAC GCA GGT GAT TGG TG- 3' |
PPARγ | 5' -TTG CTG TCA TTA TTC TCA GTG GA- 3' | 5' -GAG GAC TCA GGG TGG TTC AG- 3' |
MYH11 | 5' -GCA GCT ACA GGC TGA AAG GA- 3' | 5' -CCT CCA GCT GTT CTT CAA GG- 3' |
GAPDH | 5' -GAA AGC CTG CCG GTG ACT AA- 3' | 5' -GCC CAA TAC GAC CAA ATC AGA G- 3' |
Table 1: List of the forward and reverse primers used in the qPCR analysis: ACTA2, COL1A, and MYH11 as MYF markers; ADPR, and PPARγ as LIF markers. GAPDH serves as a housekeeping gene.