Figure 4.

GSK rectifies high salt-induced Treg instability in vivo

(A) Schematic representation of constructing Rosa-Red mouse model.

(B) Peripheral one-drop blood flow cytometry was employed to validate Rosa-Red transgenic mice.

(C) PCR was employed for genotyping Rosa-Red mice.

(D–F) The proportion of unstable Treg cells (tdTomato⁺ GFP⁻) within the total Treg population in the spleen and lymph nodes of Rosa-Red mice. Statistical significance was calculated by unpaired Student’s t test and data are represented as mean ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.