REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD3	Biolegend	Cat#300302; RRID:AB_314037
Anti-human CD28	Biolegend	Cat#302902; RRID:AB_314303
Anti-mouse CD3	Biolegend	Cat#100302; RRID:AB_312666
Anti-mouse CD28	Biolegend	Cat#102102; RRID:AB_312866
FITC anti-mouse CD4	Biolegend	Cat#100406; RRID:AB_312690
Brilliant Violet 421™ anti-mouse IL-17A	Biolegend	Cat#506926; RRID:AB_10900442
Alexa Fluor 647 anti-mouse Foxp3	Biolegend	Cat#126408; RRID:AB_1089115
PE anti-mouse CD44	Biolegend	Cat#103008; RRID:AB_312958
APC anti-mouse CD62L	Biolegend	Cat#104412; RRID:AB_313099
PE/Cy7 anti-mouse CD25	Biolegend	Cat#101916; RRID:AB_2616762
Alexa Fluor 647 anti-human Foxp3	Biolegend	Cat#320211; RRID:AB_430886
PE anti-human IL-17A	Biolegend	Cat#512306; RRID:AB_961395
Anti-PDPK1 (phospho S241)	Abcam	Cat#ab131098; RRID:AB_11159760
Anti-HK2	Abcam	Cat#ab209847; RRID:AB_2904621
Anti-LDHA	Abcam	Cat#ab52488; RRID:AB_2134961
Anti-PDPK1	Santa Cruz Biotechnology	Cat#sc-17765; RRID:AB_626657
Anti-β-Actin	Santa Cruz Biotechnology	Cat#sc-47778; RRID:AB_626632
Anti-SGK1	ABclonal	Cat#A1025; RRID:AB_2757776
Anti-SGK1 (phospho S422)	Absin	Cat#abs147953; N.A
Anti-Glut1	Proteintech	Cat#21829-1-AP; RRID:AB_10837075
Anti-FoxO1	proteintech	Cat#18592-1-AP; RRID:AB_10860103
Biological samples
RA patients' peripheral blood samples	Tongji Hospital	N/A
Healthy Controls’ peripheral blood samples	Tongji Hospital	N/A
Chemicals, peptides, and recombinant proteins
GSK2334470 (GSK)	MedChemExpress	HY-14981
Protease inhibitor cocktail	Roche	4693116001
RIPA lysis buffer	Beyotime	P0013K
Recombinant murine IL-2	Biolegend	#575402
Recombinant murine IL-6	Biolegend	#575702
Recombinant murine TGF-β	Biolegend	#763102
Recombinant human IL-2	Biolegend	#791906
Recombinant human IL-6	Biolegend	#570806
Recombinant human TGF-β	Biolegend	#583301
Anti-TNF-α (Etanercept)	MedChemExpress	HY-108847
Critical commercial assays
Mouse inflammation kit	BD Biosciences	552364
MojoSort™ Mouse CD4 Naive T cell Isolation Kit	Miltenyi Biotec	130-104-453
Annexin V-FITC/PI Apoptosis Detection Kit	Yeasen Biotechnology	40302
ECL kit	Biosharp Life Sciences	BL520A
CFSE	Thermo Fisher	C34554
Experimental models: Organisms/strains
DBA/1 mice	Jackson Laboratory	N/A
C57BL/6 mice	Jackson Laboratory	N/A
Foxp3Cre-eGFP; Rosa26flox-stop-flox-tdTomato mice (Rosa-Red mice)	Jackson Laboratory	N/A
Software and algorithms
FlowJo software (v10.5.3)	BD Biosciences	https://www.bdbiosciences.com
GraphPad Prism 5.0 software	GraphPad Software Inc.	https://www.graphpad.com/; RRID:SCR_002798
FCAP ArrayTM Software	BD Biosciences	https://www.bdbiosciences.com