Fig. 6.
Cysteine is an inhibitor of CGL. A Here, cysteine is shown to be a non-competitive inhibitor of CGL with respect to conversion of homoserine to 2-oxobutanoate. B The Dixon plot indicates that the Ki for this inhibition is 0.52 mM. The data for this study were obtained as described in the “Methods” section using 228 ng rat liver CGL (6.42 pmol) in a reaction volume of 50 µL. The data are depicted as the means of up to 9 determinations. For the sake of clarity, the SD values are not depicted but can be found in Supplementary Table 1. All plots were fitted using Prism software and have R2 values of 1.00