Fig. 9.

Cysteine induces spectral changes in CGL indicative of a reaction with PLP. Spectra of 100 µg or 2.25 nmol of human recombinant CGL in 400 µL (5.617 µM) prior to and following the addition of cysteine at a final concentration of 100 µM are shown (A). The kinetic of the changes in absorbance at 320 and 450 nm is depicted (B). The reaction was carried out in 100 mM potassium phosphate buffer (pH 7.2) and at 22 °C. Spectra were collected every 1 min and smoothed using Prism software. The raw plots are given in Supplementary Data Fig. 4