TABLE 1.

Annotation of hypothetical proteins

Gene familya	Annotation	GO function	Confidenceb
Methylobacterium_jeotgali_CDS_4118	rpmE, 50S ribosomal protein L31	Translation	Yes
Methylooceanibacter_caenitepidi_CDS_2654	yhbS, N-acetyltransferase (GNAT)	Organonitrogen compound biosynthetic process	No
Methylobacterium_phyllostachyos_CDS_4295	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex	Organophosphate metabolic process	Yes
Methylocella_tundrae_CDS_3415	EC:3.4.11.9: Xaa-Pro aminopeptidase	Metalloaminopeptidase activity	Yes
Methylocystis_hirsuta_CDS_2285	MoxR-like ATPase	Organonitrogen compound catabolic process/metalloendopeptidase activity	No
Methylooceanibacter_superfactus_CDS_2697	ATPase family	Cell cycle process	No
Methylovirgula_sp._HY1_CDS_2003	Glutamine amidotransferase	Pyrophosphatase activity	Yes
Methylobacterium_adhaesivum_CDS_1753	NAc	Cytoplasm	No
Methylovirgula_sp._4M-Z18_CDS_4185	RNA-binding domains	Lyase activity	No
Methylobacterium_terrae_CDS_3436	NA	Phosphatidylinositol phosphorylation	No
Methylosinus_trichosporium_OB3b_CDS_4175	Conserved protein YbaR	Enzyme inhibitor activity	No
Methylocystis_hirsuta_CDS_2870	Lysine decarboxylase	Quinone metabolic process	Yes
Methylopila_sp._Yamaguchi_CDS_1376	DNA polymerase III subunit	Binding	Yes
Methylooceanibacter_marginalis_CDS_2229	Type III secretion system	Nitrogen compound metabolic process	Yes
Methylocystis_parvus_CDS_0309	Polyhydroxyalkanoate synthesis repressor PhaR	rRNA processing/transcription factor binding	Yes
Methylooceanibacter_methanicus_CDS_0298	Polyhydroxyalkanoate synthesis regulator protein	rRNA processing/transcription factor binding	Yes
Methylobacterium_brachythecii_CDS_2235	Zn-finger domain	Cofactor binding	No
Methylobacterium_bullatum_CDS_2120	ATPase, AAA+	Transferase activity, transferring phosphorus-containing groups	No
Methylobrevis_albus_CDS_1240	Haloacid dehalogenase	Catalytic activity	Yes
Methylocystis_parvus_CDS_1055	SH3-like domain	Organonitrogen compound metabolic process	No
Methylobacterium_oxalidis_CDS_2397	PtsH, phosphotransferase	Carbohydrate transport	Yes
Methylocapsa_sp._S129_CDS_5161	Dipeptidyl aminopeptidase	Catalytic activity	Yes

^a Information related to the gene families with regard to the FASTA sequences used in the study are provided in Table S4.

^b The confidence level is determined by the combined score derived from motif-based protein-level prediction from PROSITE and NCBI, as well as the GO process-based ProteInfer.

^c NA: not applicable.