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. 2024 Jun 28;13(13):1120. doi: 10.3390/cells13131120

Figure 2.

Figure 2

Western blot of monoclonal cell lines SmKPP.1, CmKPP.1 and LLC. Both SmKPP.1 and CmKPP.1 exhibit typical tumor markers of human lung adenocarcinoma including the presence of proteins TTF-1 (40 kDA), NapA (35 kDA), and absence of p40 (40 kDA); they also demonstrate the absence of gene product p53 (53 kDA), and the presence of KRASG12D (25 kDA) and p110α (110 kDA). This is in comparison to LLC which is positive for NapA but negative for TTF-1 and p40, positive for p53, and negative for KRASG12D and p110α. PD-L1 is more strongly present in CmKPP.1 compared to SmKPP.1 and LLC. Murine testes (MT) serve as a positive control for p40, and human lung fibroblasts (HLF) serve as a positive control for PD-L1. Cell passage 22 used for SmKPP.1 and passage 16 for CmKPP.1.