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. 2024 Jun 28;13(13):1120. doi: 10.3390/cells13131120

Figure 3.

Figure 3

Immunofluorescence staining of normal, healthy murine lung tissue and orthotopic tumors generated with monoclonal SmKPP.1 or CmKPP.1 cells. Images demonstrate that tumors generated by SmKPP.1 are positive for TTF-1 ((B.1), red), NapA ((B.2), red), and PD-L1 ((B.3), red); tumors generated by CmKPP.1 are also positive for TTF-1 ((C.1), red), NapA ((C.2), red), and PD-L1 ((C.3), red). Lung tissue from normal control mice without tumor exhibits a low expression of TTF-1 ((A.1), arrows), and the absence of NapA (A.2) and PD-L1 (A.3). All tissue slices were stained for CD31 (green, for endothelial cells, not included in the overlay for SmKPP.1 or CmKPP.1) to provide architecture and counterstained with DAPI (blue, for cellular nuclei). The scale bar represents 50 μm.