Figure 3. Rhythmic transcripts, and their associated processes, overlap between sexes in pyramidal cells.

(A) The number of rhythmic transcripts in pyramidal cells isolated from male and female animals at different significance cutoffs. The percentage of rhythmic transcripts is listed in parentheses. Both males and females show a similar number of rhythmic transcripts. (B) Venn diagram showing the overlap of rhythmic transcripts (p<0.05) between males and females. Nearly half of all rhythmic transcripts in pyramidal cells are rhythmic in both sexes. (C) Threshold free approach (rank-rank hypergeometric overlap) assessing the overlap of rhythmicity in transcripts between males and females. There is broad overlap in rhythmic transcripts between sexes in pyramidal cells. (D) The top 10 pathways significantly enriched for rhythmic transcripts in pyramidal cells as determined by Ingenuity Pathway Analysis. The vertical line represents significant enrichment (-log₁₀(p-value)>1.3). Top pathways include multiple cancer associated pathways. (E)&(F) The top 10 pathways enriched for transcripts that are uniquely rhythmic in either male (E) or females (F), showing sex specific roles of rhythms in pyramidal cells. PYR=pyramidal cells