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. 2024 Jun 13;15(7):e01321-24. doi: 10.1128/mbio.01321-24

Fig 7.

Western blots of ZBTB20, I kappa beta alpha pathway across SCFA, JEV, and SCFA plus JEV with miR-200a-3p manipulation and quantification of protein levels with comparison between scramble, mimic, and inhibitor are featured.

miR-200a-3p-regulated ZBTB20 alters Iκβα and NF-κB protein levels downstream. (A) Representable immunoblots showing protein expression of ZBTB20, total/p-Iκβα, and total/pNF-κB in the presence of scramble/miR-200a-3p mimic in untreated, SCFA, JEV, and SCFA + JEV conditions. Each mimic condition has been compared to its scramble counterpart. (B) Bar graphs represent densitometric quantification of protein levels from panel A. (C) Representable immunoblots showing protein expression of ZBTB20, total/p-Iκβα, and total/pNF-κB in the presence of scramble/miR-200a-3p inhibitor in untreated, SCFA, JEV, and SCFA + JEV conditions. Each inhibitor condition has been compared to its scramble counterpart. (D) Bar graphs represent densitometric quantification of protein levels from panel C. Data represented in grouped form as mean fold change ± SEM with respect to scramble control in each condition. A minimum of three independent experiments were used. Scramble controls have been normalized using untreated control group. P-values were determined (*, P < 0.05;**, P < 0.01;***, P < 0.001) using two-way analysis of variance and Tukey’s post hoc correction.