TABLE 1.
Target | Sequence (5′ to 3′) | |
---|---|---|
JEV-GP78 | Sense | TTGACAATCATGGCAAAC |
Antisense | CCCAACTTGCGCTGAATAA | |
ZBTB20 | Sense | CCTCATCCACTCGACACATTCAC |
Antisense | GAAGGTTGATGCTGTGAATGCGC | |
IKβα | Sense | GCCAGGAATTGCTGAGGCACTT |
Antisense | GTCTGCGTCAAGACTGCTACAC | |
mmu-miR-122–5p | TGGAGTGTGACAATGGTGTTTG | |
mmu-miR-1946b | GCCGGGCAGTGGTGGCACATGCTTTT | |
mmu-miR-1a-3p | TGGAATGTAAAGAAGTATGTAT | |
mmu-miR-31–5p | AGGCAAGATGCTGGCATAGCTG | |
mmu-miR-499–5p | TTAAGACTTGCAGTGATGTTT | |
WT ZBTB20 | Sense | TCAGCAGAGCTCGCTACTGGCCTCTAAATACAACC |
Antisense | ACTGCTTCTAGACCCACCCTCCATTTCTTTTTCT | |
SDM ZBTB20 | Sense | ACACCGCTCACAGTTTTCAGAGAACAGTT |
Antisense | AACTGTTCTCTGAAAACTGTGAGCGGTGT | |
Plasmid-specific primer (Luc) | Sense | AAGGGCGGCAAGATCGCCGTGTAA |
WT, wild type; SDM, site-directed mutagenesis.