Table. 4 |.

Details of antibodies used for immunofluorescence.

Antibody (clone)	Species	Company	Catalog no.	IHC	Antigen retrieval	Temperature	Time
Anti-NeuN (A60)	Mouse mono	Millipore Sigma	MAB377	1:1000	20 m	4°C	o/n
Anti-NeuN	Guinea pig poly	Millipore Sigma	ABN90	1:1000	20 m	4°C	o/n
Anti-Calbindin D-28k	Rabbit	Swant Inc	CD38	1:1000	20 m	4°C	o/n
Anti-Parvalbumin	Guinea pig poly	Synaptic Systems	195 044	1:1000	20 m	4°C	o/n
Anti-RFP	Chicken poly	Rockland Immunochemicals	600-901-379	1:1000 (no antigen retrieval) 1:500 (with antigen retrieval)	No 20 m	4°C	o/n or 48 h*
Anti-MEIS2 (H-10)	Mouse mono	Santa Cruz Biotechnology	sc-515470	1:1000	20 m	4°C	o/n or 48 h*
Anti-TBR2	Rabbit poly	Abcam	ab23345	1:500	20 m	4°C	o/n
Anti-p27	Mouse	BD Biosciences	610241	1:500	20 m	4°C	o/n
Alexa Fluor 555 anti-chicken IgY (H+L)	Goat	Invitrogen	A21437	1:500	n/a	RT	1 h
Alexa Fluor 488 anti-rabbit IgG (H+L)	Donkey	Invitrogen	A21206	1:500	n/a	RT	1 h
Alexa Fluor 647 anti-rabbit IgG (H+L)	Donkey	Invitrogen	A31573	1:500	n/a	RT	1 h or 2 h*
Alexa Fluor 488 anti-mouse IgG (H+L)	Donkey	Invitrogen	A21202	1:500	n/a	RT	1 h
Alexa Fluor 647 anti-mouse IgG (H+L)	Donkey	Invitrogen	A31571	1:500	n/a	RT	1 h or 2 h*
Alexa Fluor 647 anti-guinea pig IgG (H+L)	Goat	Invitrogen	A21450	1:500	n/a	RT	1 h
Anti-mouse IgG antibody (H+L), Biotinylated	Goat	Vector Laboratories	BA-9200	1:500	n/a	RT	1 h
Anti-chicken IgG antibody (H+L), Biotinylated	Goat	Vector Laboratories	BA-9010	1:500	n/a	RT	1 h

^*Applicable for free-floating immunofluorescence or immunohistochemistry