Fig. 3.

Subtypes and development trajectory of Macrophage

A) Application CytoTRACE to dissect Macrophage differentiation

B) Boxplots showing CytoTRACE values for 3 Macrophage subtype

C)The 10 genes with the highest positive and negative correlation with CAF development were calculated by Cytotrace, The horizontal axis shows the correlation, the vertical axis shows the names of the genes, and the colors of the bar chart are red and purple, respectively, indicating positive and negative correlations.

D)The 10 genes negatively correlated with cell development in C) were displayed in dotplot to check their expression in specific cell clusters across 3 different tumor samples

G) BEAM analysis in Monocle2 indicates different expression patterns along the development of Macrophage to different Macrophage subtype fate.

H) Relative expression of GNLY, KLRD1,COL1A2,HLA-DQA2, CMC1 and GZMK in Macrophage trajectory branches.