In the published article, there was an error in Figure 3 as published. The western blot images of pACLY/ACLY ( Figure 3A ) were also mistakenly inserted in Figure 3B in place of pPRAS40/PRAS40 images. The corrected Figure 3 and its caption appear below.
Figure 3.
Effects of Akt and PKA inhibition on ACLY phosphorylation, LPS-induced pro-inflammatory cytokine production and basal histone acetylation in THP-1 cells. (A) Western blot analysis of ACLY phosphorylation in ACLY WT cells upon treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. (B) Western blot analysis of PRAS40 phosphorylation in ACLY WT cells upon treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. (C) Western blot analysis of CREB phosphorylation upon treatment with 10 µM H-89 or 50 µM forskolin for 30 minutes (D) mRNA expression of IL6, CCL2 and CCL4 in ACLY WT and S455A THP-1 cells following treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes and 100 ng/ml LPS for 3 hours. (E) Western blot analysis of histone H3 acetylation at K9, K27, K14 and K23 in ACLY WT and S455A cells after treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. Data represent mean values ± SE of 3-7 independent experiments. * p<0.05, ** p<0.01, *** p<0.001.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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