Main text
(iScience 26, 106444; April 21, 2023)
During figure preparation for this article, the authors made errors in composing the PLA images in Figures 2, 5, S1, and S3. The changes are detailed below.
In Figure 2A, the original representative 4 hr p53/PARP1 PLA image was of a different cell than the one shown in the 4 hr DAPI and Merge images. We have now included the PLA image for the same cell as the one shown in the DAPI and Merge images. In Figures 2A–2D, the representative images for DAPI and Merge were swapped. The order has now been corrected. In Figure 5A, the representative 45 m DTX3L KO#1 images (PLA, DAPI, and Merge) for DTX3L/UbiK48 were erroneously duplicated from the Figure 3A 45 m DTX3L KO#1 images (PLA, DAPI, and Merge) for p53/DTX3L. The authors have now substituted the correct representative images. In Figure S1C, the representative 4 m and 8 m ABT888 images (PLA, DAPI, and Merge) for PARP1/PAR were erroneously duplicated from the 15 m and 0 m ABT888 images (PLA, DAPI, and Merge) for PARP1/PAR, respectively. The authors have now substituted the correct representative images. In Figure S3A, the representative 45 m DTX3L KO#2 images (PLA, DAPI, and Merge) for DTX3L/UbiK48 were erroneously duplicated from the Figure 3A 45 m DTX3L KO#2 images (PLA, DAPI, and Merge) for p53/DTX3L. The authors have now substituted the correct representative images. The authors note that the aggregate data, which were directly derived from the Leica Application Suite X (LAS X) software as quantified PLA mean nuclear intensities and shown as bar graphs of “mean nuclear intensity” in Figures 2E, 5B, and S1C (bottom panel), are unchanged, and the conclusions of these studies remain the same. The authors apologize to the readers.
Figure 2. P53 is recruited to PARP-decorated DNA damage sites and retained at these sites by chemical PARP inhibition or DTX3L depletion (original)
Figure 2. P53 is recruited to PARP-decorated DNA damage sites and retained at these sites by chemical PARP inhibition or DTX3L depletion (corrected)
Figure 5. DTX3L is required for p53 K48-linked polyubiquitylation at DNA damage foci (original)
Figure 5. DTX3L is required for p53 K48-linked polyubiquitylation at DNA damage foci (corrected)
Figure S1. Chemical PARP inhibition increases p53/PARP1 co-localization at DNA damage sites, related to Figure 2 (original)
Figure S1. Chemical PARP inhibition increases p53/PARP1 co-localization at DNA damage sites, related to Figure 2 (corrected)
Figure S3. K48-linked polyubiquitylation of p53 is DTX3L-dependent, related to Figure 5 (original)
Figure S3. K48-linked polyubiquitylation of p53 is DTX3L-dependent, related to Figure 5 (corrected)