TABLE 2.
Family, year(s) reported, sequencing statistics, mosquito species, and Genbank accession numbers for each novel virus
| Viral family | Virus | Year(s) reported |
No. of contigs | Longest contig (nt) | Coverage depth (mean) | % Identity (median)a | GenBank accession numbers |
Species (% sequencing libraries)b |
|---|---|---|---|---|---|---|---|---|
| Dicistroviridae | Manitoba dicistro-like virus 1 | 2019 | 1 | 7,146 | 149.27 | 82.61 | OR448845 a | Cx. tarsalis (9.09) |
| Iflaviridae | Manitoba iflavirus 1 | 2020 and 2021 | 13 | 2,435 | 78.09 | 79.47 | OR448846 and OR448915–https://www.ncbi.nlm.nih.gov/nuccore/OR448915;https://www.ncbi.nlm.nih.gov/nuccore/OR448916;https://www.ncbi.nlm.nih.gov/nuccore/OR448917;https://www.ncbi.nlm.nih.gov/nuccore/OR448918;https://www.ncbi.nlm.nih.gov/nuccore/OR448919;https://www.ncbi.nlm.nih.gov/nuccore/OR448920;https://www.ncbi.nlm.nih.gov/nuccore/OR448921;https://www.ncbi.nlm.nih.gov/nuccore/OR448922;https://www.ncbi.nlm.nih.gov/nuccore/OR448923;https://www.ncbi.nlm.nih.gov/nuccore/OR448924;https://www.ncbi.nlm.nih.gov/nuccore/OR448925; OR448925 | Aae. vexans (47.37) and Oc. dorsalis (20) |
| Iflaviridae | Manitoba picorna-like virus 1 | 2019–2021 | 20 | 2,114 | 70.96 | 83.1 | OR448852 and OR448928– OR448946 | Ae. vexans (63.16) |
| Narnaviridae | Manitoba narnavirus 1 | 2021 | 2 | 1,760 | 18.77 | 80.59 | OR448851 and OR448927 | An. earlei (100) |
| Negevirus | Manitoba mononega-like virus 1 | 2021 | 1 | 11,180 | 59.07 | 73.21 | OR448848 | Ae. canadensis (100) |
| Negevirus | Manitoba mononega-like virus 2 | 2021 | 1 | 2,477 | 20.03 | 75 | OR448849 | Ae. canadensis (100) |
| Negevirus | Manitoba mononega-like virus 3 | 2020 | 1 | 5,456 | 42.65 | 78.57 | OR448850 | Oc. triseriatus (100) |
| Tombusviridae | Manitoba tombus-like virus 1 | 2019–2021 | 5 | 2,340 | 157.57 | 76.19 | OR448856 and OR448970– OR448973 | Cx. tarsalis (45.45) |
| Tymoviridae | Manitoba tymo-like virus 1 | 2021 | 1 | 6,302 | 23.67 | 66.79 | OR448860 | Ae. vexans (5.26) |
| Virgaviridae | Manitoba virgavirus 1 | 2020 | 1 | 1,675 | 11.06 | 83.83 | OR448861 | Oc. dorsalis (20) |
| Rhabdoviridae | Manitoba Rhabdovirus 1 | 2019–2021 | 20 | 5,085 | 26.41 | 77.9 | OR448853 and OR448947–OR448965 | Ae. vexans (73.68) and Oc. dorsalis (20) |
| Rhabdoviridae | Manitoba rhabdovirus 2 | 2020 | 1 | 4,648 | 51.7 | 75 | OR448854 | Ae. vexans (5.26) |
| Rhabdoviridae | Manitoba rhabdovirus 3 | 2020 and 2021 | 5 | 6,071 | 16.51 | 84.62 | OR448855 and OR448966–OR448969 | Ae. canadensis (100) and Cx. tarsalis (36.36) |
| Iridoviridae | Manitoba iridescent virus 1 | 2021 | 1 | 797 | 16.99 | 78.74 | OR448847 | Cq. perturbans (16.67) |
| Totiviridae | Manitoba toti-like virus 1 | 2019–2021 | 5 | 8,930 | 39.82 | 68.1 | OR448857 and OR448974–OR448977 | Ae. vexans (10.53) and Cx. tarsalis (27.27) |
| Totiviridae | Manitoba toti-like virus 2 | 2021 | 1 | 3,123 | 13.45 | 60.24 | OR448858 | Ae. canadensis (100) |
| Totiviridae | Manitoba toti-like virus 3 | 2020 | 4 | 4,626 | 15.11 | 73.5 | OR448859 and OR448978–OR448980 | Oc. dorsalis (80) |
a
All contig sequences for a given virus had amino acid (aa) identities of <85% in the NCBI databases.
b
Proportion of mosquito cDNA libraries (by species) in which a given virus was detected.