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. 1983 Jul;72(1):128–141. doi: 10.1172/JCI110950

Endothelial characteristics of glomerular capillaries in normal, mercuric chloride-induced, and gentamicin-induced acute renal failure in the rat.

R E Bulger, G Eknoyan, D J Purcell 2nd, D C Dobyan
PMCID: PMC1129168  PMID: 6223941

Abstract

A reduction in glomerular capillary endothelial pore size and density has been reported in several models of acute renal failure. It has been suggested that these changes underlie the decrease in glomerular filtration rate and altered glomerular capillary hemodynamics measured in various experimental models of acute renal failure. We have thoroughly quantitated the surface characteristics of glomerular capillaries in control rats and in rats with either mercuric chloride-induced acute renal failure (2 mg/kg body wt) evaluated at 6 and 24 h after administration of the nephrotoxin or with gentamicin (G)1-induced acute renal failure evaluated after 8-9 d of 40 mg/kg body wt twice a day. Despite reductions in glomerular filtration rate in the experimental groups, no significant differences were observed between control (C) and any experimental group with respect to percent areas occupied by fenestrated endothelium (C = 53.6 +/- 2.7%; 6 h HgCl2 = 50.9 +/- 1.9%; 24 h HgCl2 = 53.9 +/- 5.7%; G = 56.7 +/- 2.4%), by cytoplasmic ridges (C = 31.2 +/- 1.5%; 6 h HgCl2 = 29.8 +/- 1.9%; 24 h HgCl2 = 30.6 +/- 3.1%; G = 26.5 +/- 1.5%), nonfenestrated endothelium (C = 15.5 +/- 4.0%; 6 h HgCl2 = 19.3 +/- 2.0%; 24 h HgCl2 = 15.6 +/- 4.3%; G = 16.9 +/- 2.3%), in the individual pore area expressed in square nanometers (C = 1,494 +/- 75; 6 h HgCl2 = 1,326 +/- 48; 24 h HgCl2 = 1,559 +/- 130; G = 1,340 +/- 101), or in the percentage of total pore area within fenestrated areas that were measured (C = 12.8 +/- 0.8%; 6 h HgCl2 = 11.2 +/- 0.7%; 24 h HgCl2 = 10.9 +/- 0.8%; G = 10.9 +/- 0.7%). These results provide quantitative data on the normal glomerular capillary endothelial surface characteristics and suggest that reductions of glomerular filtration rate in acute renal failure are not always associated with alterations in glomerular endothelial capillaries.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avasthi P. S., Evan A. P. Glomerular permeability in aminonucleoside-induced nephrosis in rats. A proposed role of endothelial cells. J Lab Clin Med. 1979 Feb;93(2):266–276. [PubMed] [Google Scholar]
  2. Avasthi P. S., Evan A. P., Hay D. Glomerular endothelial cells in uranyl nitrate-induced acute renal failure in rats. J Clin Invest. 1980 Jan;65(1):121–127. doi: 10.1172/JCI109641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avasthi P. S., Evan A. P., Huser J. W., Luft F. C. Effect of gentamicin on glomerular ultrastructure. J Lab Clin Med. 1981 Sep;98(3):444–454. [PubMed] [Google Scholar]
  4. Baehler R. W., Kotchen T. A., Burke J. A., Galla J. H., Bhathena D. Considerations on the pathophysiology of mercuric chloride-induced acute renal failure. J Lab Clin Med. 1977 Aug;90(2):330–340. [PubMed] [Google Scholar]
  5. Baylis C., Rennke H. R., Brenner B. M. Mechanisms of the defect in glomerular ultrafiltration associated with gentamicin administration. Kidney Int. 1977 Nov;12(5):344–353. doi: 10.1038/ki.1977.121. [DOI] [PubMed] [Google Scholar]
  6. DiBona G. F., McDonald F. D., Flamenbaum W., Dammin G. J., Oken D. E. Maintenance of renal function in salt loaded rats despite severe tubular necrosis induced by HgCl 2 . Nephron. 1971;8(3):205–220. doi: 10.1159/000179922. [DOI] [PubMed] [Google Scholar]
  7. Eknoyan G., Bulger R. E., Dobyan D. C. Mercuric chloride-induced acute renal failure in the rat. I. Correlation of functional and morphologic changes and their modification by clonidine. Lab Invest. 1982 Jun;46(6):613–620. [PubMed] [Google Scholar]
  8. Fujita T., Tokunaga J., Edanaga M. Scanning electron microscopy of the glomerular filtration membrane in the rat kidney. Cell Tissue Res. 1976 Feb 25;166(3):299–314. doi: 10.1007/BF00220127. [DOI] [PubMed] [Google Scholar]
  9. Griffith L. D., Bulger R. E., Trump B. F. The ultrastructure of the functioning kidney. Lab Invest. 1967 Feb;16(2):220–246. [PubMed] [Google Scholar]
  10. Jamison R. L. Micropuncture study of superficial and juxtamedullary nephrons in the rat. Am J Physiol. 1970 Jan;218(1):46–55. doi: 10.1152/ajplegacy.1970.218.1.46. [DOI] [PubMed] [Google Scholar]
  11. Keane W. F., Raij L. Determinants of glomerular mesangial localization of immune complexes. Role of endothelial fenestrae. Lab Invest. 1981 Oct;45(4):366–371. [PubMed] [Google Scholar]
  12. Luft F. C., Aronoff G. R., Evan A. P., Connors B. A. The effect of aminoglycosides on glomerular endothelium: a comparative study. Res Commun Chem Pathol Pharmacol. 1981 Oct;34(1):89–95. [PubMed] [Google Scholar]
  13. Luft F. C., Evan A. P. Comparative effects of tobramycin and gentamicin on glomerular ultrastructure. J Infect Dis. 1980 Dec;142(6):910–914. doi: 10.1093/infdis/142.6.910. [DOI] [PubMed] [Google Scholar]
  14. Luft F. C., Evan A. P. Glomerular filtration barrier in aminoglycoside-induced nephrotoxic acute renal failure. Ren Physiol. 1980;3(1-6):265–271. doi: 10.1159/000172770. [DOI] [PubMed] [Google Scholar]
  15. Olson J. L., Rennke H. G., Venkatachalam M. A. Alterations in the charge and size selectivity barrier of the glomerular filter in aminonucleoside nephrosis in rats. Lab Invest. 1981 Mar;44(3):271–279. [PubMed] [Google Scholar]
  16. Schor N., Ichikawa I., Rennke H. G., Troy J. L., Brenner B. M. Pathophysiology of altered glomerular function in aminoglycoside-treated rats. Kidney Int. 1981 Feb;19(2):288–296. doi: 10.1038/ki.1981.19. [DOI] [PubMed] [Google Scholar]
  17. Solez K., Racusen L. C., Whelton A. Glomerular epithelial cell changes in early postischemic acute renal failure in rabbits and man. Am J Pathol. 1981 May;103(2):163–173. [PMC free article] [PubMed] [Google Scholar]
  18. Tokunaga J., Edanaga M., Fujita T., Adachi K. Freeze cracking of scanning electron microscope specimens. A study of the kidney and spleen. Arch Histol Jpn. 1974 Sep;37(2):165–182. doi: 10.1679/aohc1950.37.165. [DOI] [PubMed] [Google Scholar]
  19. Whelton A., Solez K. Aminoglycoside nephrotoxicity--a tale of two transports. J Lab Clin Med. 1982 Feb;99(2):148–155. [PubMed] [Google Scholar]
  20. Williams R. H., Thomas C. E., Navar L. G., Evan A. P. Hemodynamic and single nephron function during the maintenance phase of ischemic acute renal failure in the dog. Kidney Int. 1981 Apr;19(4):503–515. doi: 10.1038/ki.1981.48. [DOI] [PubMed] [Google Scholar]

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