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. 1992 Feb 15;282(Pt 1):203–207. doi: 10.1042/bj2820203

Damage-recognition proteins as a potential indicator of DNA-damage-mediated sensitivity or resistance of human cells to ultraviolet radiation.

C C Chao 1
PMCID: PMC1130908  PMID: 1540136

Abstract

We have previously identified damage-recognition proteins that bind to cisplatin[cis-diamminedichloroplatinum(II), a DNA cross-linking agent]- or u.v.-modified DNA in HeLa cells [Chao, Huang, Huang & Lin-Chao (1991) Mol. Cell. Biol. 11, 2075-2080; Chao, Huang, Lee & Lin-Chao (1991) Biochem. J. 277, 875-878]. In the present study we compared damage-recognition proteins in cells expressing different sensitivities to DNA damage. An increase in damage-recognition proteins and an enhancement of plasmid re-activation were detected in HeLa cells resistant to cisplatin and u.v. However, repair-defective cells derived from xeroderma-pigmentosum (a rare skin disease) patients did not express less cisplatin damage-recognition proteins than repair-competent cells, suggesting that damage-recognition-protein expression may not be related to DNA repair. By contrast, cells resistant to DNA damage consistently expressed high levels of u.v.-modified-DNA damage-recognition proteins. The results support the notion that u.v. damage-recognition proteins are different from those that bind to cisplatin. These findings also suggest that the damage-recognition proteins identified could be used as potential indicators of the sensitivity or resistance of cells to u.v.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Behrens B. C., Hamilton T. C., Masuda H., Grotzinger K. R., Whang-Peng J., Louie K. G., Knutsen T., McKoy W. M., Young R. C., Ozols R. F. Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues. Cancer Res. 1987 Jan 15;47(2):414–418. [PubMed] [Google Scholar]
  2. Bird P., Gething M. J., Sambrook J. Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent. J Cell Biol. 1987 Dec;105(6 Pt 2):2905–2914. doi: 10.1083/jcb.105.6.2905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Carthew R. W., Chodosh L. A., Sharp P. A. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell. 1985 Dec;43(2 Pt 1):439–448. doi: 10.1016/0092-8674(85)90174-6. [DOI] [PubMed] [Google Scholar]
  5. Chao C. C., Huang S. L., Huang H. M., Lin-Chao S. Cross-resistance to UV radiation of a cisplatin-resistant human cell line: overexpression of cellular factors that recognize UV-modified DNA. Mol Cell Biol. 1991 Apr;11(4):2075–2080. doi: 10.1128/mcb.11.4.2075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chao C. C., Huang S. L., Lee L. Y., Lin-Chao S. Identification of inducible damage-recognition proteins that are overexpressed in HeLa cells resistant to cis-diamminedichloroplatinum (II). Biochem J. 1991 Aug 1;277(Pt 3):875–878. doi: 10.1042/bj2770875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chao C. C., Lee Y. L., Cheng P. W., Lin-Chao S. Enhanced host cell reactivation of damaged plasmid DNA in HeLa cells resistant to cis-diamminedichloroplatinum(II). Cancer Res. 1991 Jan 15;51(2):601–605. [PubMed] [Google Scholar]
  8. Chao C. C., Lee Y. L., Lin-Chao S. Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid. Biochem Biophys Res Commun. 1990 Jul 31;170(2):851–859. doi: 10.1016/0006-291x(90)92169-z. [DOI] [PubMed] [Google Scholar]
  9. Chao C. C., Lin-Chao S. Transient induction of photolyase activity in arrested frog cells in response to a short-wave ultraviolet segment of simulated "sunlight". Biochem Biophys Res Commun. 1987 May 29;145(1):604–611. doi: 10.1016/0006-291x(87)91363-5. [DOI] [PubMed] [Google Scholar]
  10. Chao C. C., Rosenstein B. S. Induction of sister-chromatid exchanges in ICR 2A frog cells exposed to 254 nm and solar UV wavelengths. Photochem Photobiol. 1985 May;41(5):625–627. doi: 10.1111/j.1751-1097.1985.tb03537.x. [DOI] [PubMed] [Google Scholar]
  11. Chu G., Chang E. Cisplatin-resistant cells express increased levels of a factor that recognizes damaged DNA. Proc Natl Acad Sci U S A. 1990 May;87(9):3324–3327. doi: 10.1073/pnas.87.9.3324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Chu G., Chang E. Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA. Science. 1988 Oct 28;242(4878):564–567. doi: 10.1126/science.3175673. [DOI] [PubMed] [Google Scholar]
  13. Dignam J. D., Lebovitz R. M., Roeder R. G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983 Mar 11;11(5):1475–1489. doi: 10.1093/nar/11.5.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Donahue B. A., Augot M., Bellon S. F., Treiber D. K., Toney J. H., Lippard S. J., Essigmann J. M. Characterization of a DNA damage-recognition protein from mammalian cells that binds specifically to intrastrand d(GpG) and d(ApG) DNA adducts of the anticancer drug cisplatin. Biochemistry. 1990 Jun 19;29(24):5872–5880. doi: 10.1021/bi00476a032. [DOI] [PubMed] [Google Scholar]
  15. Eastman A., Schulte N. Enhanced DNA repair as a mechanism of resistance to cis-diamminedichloroplatinum(II). Biochemistry. 1988 Jun 28;27(13):4730–4734. doi: 10.1021/bi00413a022. [DOI] [PubMed] [Google Scholar]
  16. Fraval H. N., Roberts J. J. Excision repair of cis-diamminedichloroplatinum(II)-induced damage to DNA of Chinese hamster cells. Cancer Res. 1979 May;39(5):1793–1797. [PubMed] [Google Scholar]
  17. Frei E., 3rd, Cucchi C. A., Rosowsky A., Tantravahi R., Bernal S., Ervin T. J., Ruprecht R. M., Haseltine W. A. Alkylating agent resistance: in vitro studies with human cell lines. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2158–2162. doi: 10.1073/pnas.82.7.2158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Fry D. W., Jackson R. C. Membrane transport alterations as a mechanism of resistance to anticancer agents. Cancer Surv. 1986;5(1):47–79. [PubMed] [Google Scholar]
  19. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., Howard B. H. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777–6781. doi: 10.1073/pnas.79.22.6777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hamilton T. C., Winker M. A., Louie K. G., Batist G., Behrens B. C., Tsuruo T., Grotzinger K. R., McKoy W. M., Young R. C., Ozols R. F. Augmentation of adriamycin, melphalan, and cisplatin cytotoxicity in drug-resistant and -sensitive human ovarian carcinoma cell lines by buthionine sulfoximine mediated glutathione depletion. Biochem Pharmacol. 1985 Jul 15;34(14):2583–2586. doi: 10.1016/0006-2952(85)90551-9. [DOI] [PubMed] [Google Scholar]
  22. Hirschfeld S., Levine A. S., Ozato K., Protić M. A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells. Mol Cell Biol. 1990 May;10(5):2041–2048. doi: 10.1128/mcb.10.5.2041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hospers G. A., Mulder N. H., De Vries E. G. Mechanisms of cellular resistance to cisplatin. Med Oncol Tumor Pharmacother. 1988;5(3):145–151. doi: 10.1007/BF02986437. [DOI] [PubMed] [Google Scholar]
  24. Jones J. C., Zhen W. P., Reed E., Parker R. J., Sancar A., Bohr V. A. Gene-specific formation and repair of cisplatin intrastrand adducts and interstrand cross-links in Chinese hamster ovary cells. J Biol Chem. 1991 Apr 15;266(11):7101–7107. [PubMed] [Google Scholar]
  25. Lai G. M., Ozols R. F., Smyth J. F., Young R. C., Hamilton T. C. Enhanced DNA repair and resistance to cisplatin in human ovarian cancer. Biochem Pharmacol. 1988 Dec 15;37(24):4597–4600. doi: 10.1016/0006-2952(88)90325-5. [DOI] [PubMed] [Google Scholar]
  26. Loehrer P. J., Einhorn L. H. Drugs five years later. Cisplatin. Ann Intern Med. 1984 May;100(5):704–713. doi: 10.7326/0003-4819-100-5-704. [DOI] [PubMed] [Google Scholar]
  27. Masuda H., Ozols R. F., Lai G. M., Fojo A., Rothenberg M., Hamilton T. C. Increased DNA repair as a mechanism of acquired resistance to cis-diamminedichloroplatinum (II) in human ovarian cancer cell lines. Cancer Res. 1988 Oct 15;48(20):5713–5716. [PubMed] [Google Scholar]
  28. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
  29. Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Patterson M., Chu G. Evidence that xeroderma pigmentosum cells from complementation group E are deficient in a homolog of yeast photolyase. Mol Cell Biol. 1989 Nov;9(11):5105–5112. doi: 10.1128/mcb.9.11.5105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Plooy A. C., van Dijk M., Lohman P. H. Induction and repair of DNA cross-links in chinese hamster ovary cells treated with various platinum coordination compounds in relation to platinum binding to DNA, cytotoxicity, mutagenicity, and antitumor activity. Cancer Res. 1984 May;44(5):2043–2051. [PubMed] [Google Scholar]
  32. Rosenberg B. Fundamental studies with cisplatin. Cancer. 1985 May 15;55(10):2303–23l6. doi: 10.1002/1097-0142(19850515)55:10<2303::aid-cncr2820551002>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  33. Rosenstein B. S., Chao C. C. Isolation of a mutant cell line derived from ICR 2A frog cells hypersensitive to the induction of non-dimer DNA damage by solar ultraviolet radiation. Somat Cell Mol Genet. 1985 Jul;11(4):339–344. doi: 10.1007/BF01534410. [DOI] [PubMed] [Google Scholar]
  34. Sebastian J., Kraus B., Sancar G. B. Expression of the yeast PHR1 gene is induced by DNA-damaging agents. Mol Cell Biol. 1990 Sep;10(9):4630–4637. doi: 10.1128/mcb.10.9.4630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sheibani N., Jennerwein M. M., Eastman A. DNA repair in cells sensitive and resistant to cis-diamminedichloroplatinum(II): host cell reactivation of damaged plasmid DNA. Biochemistry. 1989 Apr 4;28(7):3120–3124. doi: 10.1021/bi00433a055. [DOI] [PubMed] [Google Scholar]
  36. Tyrrell R. M. Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage. Proc Natl Acad Sci U S A. 1984 Feb;81(3):781–784. doi: 10.1073/pnas.81.3.781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Ushay H. M., Tullius T. D., Lippard S. J. Inhibition of the BamHI cleavage and unwinding of pBR322 deoxyribonucleic acid by the antitumor drug cis-dichlorodiammineplatinum(II). Biochemistry. 1981 Jun 23;20(13):3744–3748. doi: 10.1021/bi00516a012. [DOI] [PubMed] [Google Scholar]
  38. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  39. Zwelling L. A., Anderson T., Kohn K. W. DNA-protein and DNA interstrand cross-linking by cis- and trans-platinum(II) diamminedichloride in L1210 mouse leukemia cells and relation to cytotoxicity. Cancer Res. 1979 Feb;39(2 Pt 1):365–369. [PubMed] [Google Scholar]
  40. Zwelling L. A., Michaels S., Schwartz H., Dobson P. P., Kohn K. W. DNA cross-linking as an indicator of sensitivity and resistance of mouse L1210 leukemia to cis-diamminedichloroplatinum(II) and L-phenylalanine mustard. Cancer Res. 1981 Feb;41(2):640–649. [PubMed] [Google Scholar]

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