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. 1992 May 1;283(Pt 3):807–811. doi: 10.1042/bj2830807

Isolation of different high-Mr mucin species from human whole saliva.

E C Veerman 1, P A van den Keybus 1, M Valentijn-Benz 1, A V Nieuw Amerongen 1
PMCID: PMC1130958  PMID: 1590770

Abstract

By using CsCl-density-gradient ultracentrifugation, two high-Mr mucin species were isolated from human whole saliva, having buoyant densities in 0.2 M-guanidinium chloride of approx. 1.56 g/ml (pool IA) and 1.48 g/ml (pool IIA). Analytical density-gradient centrifugation of submandibular, sublingual, labial and palatal saliva, followed by immunochemical analysis with anti-mucin monoclonal antibodies, indicated immunochemical and physicochemical similarities between the high-density mucins of pool IA and mucins from palatal salivary glands. Chemical analysis indicated that the putative palatal mucin was rich in sulphate, but poor in sialic acid. The lower-density mucins of pool IIA equated with the high-Mr mucins of submandibular-sublingual saliva, both immunochemically and physicochemically (buoyant density).

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Selected References

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