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. 1992 Apr 1;283(Pt 1):243–246. doi: 10.1042/bj2830243

Cu(2+)-heparin interaction studied by polarimetry.

D Grant 1, W F Long 1, C F Moffat 1, F B Williamson 1
PMCID: PMC1131020  PMID: 1567372

Abstract

Plots analogous to isothermal binding curves were derived from polarimetric studies of solutions of Na(+)-heparin to which Cu2+ was added. The shape of the plots suggests that discrete Cu(2+)-heparin complexes may form, the nature and stoichiometry of which depend upon the conditions under which the interaction occurs. Initially, complexes were formed with a stoichiometry of about 1 Cu2+ ion per heparin tetrasaccharide unit. Under these conditions, the complex may exist as a colloid-like phase that is not subject to simple solution equilibrium processes, and that is maintained by forces in addition to electrostatic ones. Further addition of Cu2+ resulted in the formation of a different complex with a stoichiometry of about 1 Cu2+ ion per disaccharide unit.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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