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. 1990 Mar 15;266(3):749–755. doi: 10.1042/bj2660749

Vitamin K-dependent carboxylation. Mechanistic studies with 3-fluoroglutamate-containing substrates.

A Vidal-Cros 1, M Gaudry 1, A Marquet 1
PMCID: PMC1131203  PMID: 2327963

Abstract

The tripeptides t-butyloxycarbonyl-Xaa-Glu-[3H]Val, where Xaa is either (2R,3S)- or (2R,3R)-3-fluoroglutamate (respectively the erythro and the threo isomer), were synthesized and their behaviour during vitamin K-dependent carboxylation was studied. Neither peptide was carboxylated. The erythro compound gave rise to an HF-elimination product representing 1% of the starting material. This HF elimination did not occur during incubation of the threo compound. The formation of the dehydropeptide, probably by elimination of an F- anion from an intermediate carbanion, favours the ionic pathway for vitamin K-dependent carboxylation.

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Selected References

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