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. 1990 Mar 15;266(3):757–763. doi: 10.1042/bj2660757

Menstrual-cycle-dependent expression of keratan sulphate in human endometrium.

M E Hoadley 1, M W Seif 1, J D Aplin 1
PMCID: PMC1131204  PMID: 1691631

Abstract

Immunochemical methods have been used to detect and characterize two classes of polypeptide-associated keratan sulphate (KS) in epithelial secretions from human endometrium. Monoclonal antibody D9B1 binds to a hormonally regulated sialylated epitope associated with KS in a high relative molecular mass (250,000-350,000) component that bands as a doublet in SDS/PAGE. These KS chain(s) are sensitive to keratanase, endo-beta-galactosidase and N-glycanase. A second, more highly sulphated, type of KS is also present, that is resistant to all three enzymes. This can be detected using monoclonal antibody 5D4. It is present throughout the menstrual cycle and is associated principally with a component of Mr 140,000. Thus secretory KS contributes to the environment of the implanting embryo, may be used as a molecular index of endometrial function and could be important in the establishment of pregnancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aplin J. D., Seif M. W. Basally located epithelial cell surface component identified by a novel monoclonal antibody technique. Exp Cell Res. 1985 Oct;160(2):550–555. doi: 10.1016/0014-4827(85)90202-2. [DOI] [PubMed] [Google Scholar]
  2. Bell S. C., Patel S. R., Jackson J. A., Waites G. T. Major secretory protein of human decidualized endometrium in pregnancy is an insulin-like growth factor-binding protein. J Endocrinol. 1988 Aug;118(2):317–328. doi: 10.1677/joe.0.1180317. [DOI] [PubMed] [Google Scholar]
  3. Choi H. U., Meyer K. The structure of keratan sulphates from various sources. Biochem J. 1975 Dec;151(3):543–553. doi: 10.1042/bj1510543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Funderburgh J. L., Caterson B., Conrad G. W. Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan. J Biol Chem. 1987 Aug 25;262(24):11634–11640. [PubMed] [Google Scholar]
  5. Julkunen M., Koistinen R., Aalto-Setälä K., Seppälä M., Jänne O. A., Kontula K. Primary structure of human insulin-like growth factor-binding protein/placental protein 12 and tissue-specific expression of its mRNA. FEBS Lett. 1988 Aug 29;236(2):295–302. doi: 10.1016/0014-5793(88)80041-3. [DOI] [PubMed] [Google Scholar]
  6. Mathews M. B., Cifonelli J. A. Comparative biochemistry of keratosulfates. J Biol Chem. 1965 Nov;240(11):4140–4145. [PubMed] [Google Scholar]
  7. Mehmet H., Scudder P., Tang P. W., Hounsell E. F., Caterson B., Feizi T. The antigenic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series. Eur J Biochem. 1986 Jun 2;157(2):385–391. doi: 10.1111/j.1432-1033.1986.tb09680.x. [DOI] [PubMed] [Google Scholar]
  8. Nakazawa K., Suzuki S. Purification of Keratan Sulfate-endogalactosidase and its action on keratan sulfates of different origin. J Biol Chem. 1975 Feb 10;250(3):912–917. [PubMed] [Google Scholar]
  9. Ritvos O., Ranta T., Jalkanen J., Suikkari A. M., Voutilainen R., Bohn H., Rutanen E. M. Insulin-like growth factor (IGF) binding protein from human decidua inhibits the binding and biological action of IGF-I in cultured choriocarcinoma cells. Endocrinology. 1988 May;122(5):2150–2157. doi: 10.1210/endo-122-5-2150. [DOI] [PubMed] [Google Scholar]
  10. SENO N., MEYER K., ANDERSON B., HOFFMAN P. VARIATIONS IN KERATOSULFATES. J Biol Chem. 1965 Mar;240:1005–1010. [PubMed] [Google Scholar]
  11. Scudder P., Uemura K., Dolby J., Fukuda M. N., Feizi T. Isolation and characterization of an endo-beta-galactosidase from Bacteroides fragilis. Biochem J. 1983 Aug 1;213(2):485–494. doi: 10.1042/bj2130485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seif M. W., Aplin J. D., Buckley C. H. Luteal phase defect: the possibility of an immunohistochemical diagnosis. Fertil Steril. 1989 Feb;51(2):273–279. doi: 10.1016/s0015-0282(16)60490-9. [DOI] [PubMed] [Google Scholar]
  13. Seif M. W., Aplin J. D., Foden L. J., Tindall V. R. A novel approach for monitoring the endometrial cycle and detecting ovulation. Am J Obstet Gynecol. 1989 Feb;160(2):357–362. doi: 10.1016/0002-9378(89)90444-4. [DOI] [PubMed] [Google Scholar]
  14. Smith R. A., Seif M. W., Rogers A. W., Li T. C., Dockery P., Cooke I. D., Aplin J. D. The endometrial cycle: the expression of a secretory component correlated with the luteinizing hormone peak. Hum Reprod. 1989 Apr;4(3):236–242. doi: 10.1093/oxfordjournals.humrep.a136878. [DOI] [PubMed] [Google Scholar]

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