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[Preprint]. 2024 Aug 3:2024.08.02.606264. [Version 1] doi: 10.1101/2024.08.02.606264

Figure 7. Systemic sympathetic signaling drives ARS-enhancement of PV-BS inhibition in females.

Figure 7

(A) A Cre-dependent ChIEF AVV was injected into the vSUB of PV-Cre female mice and optogenetically evoked IPSCs from PV interneurons were recorded from BS cells 24-hr after saline, sympathetic block (symp), or corticosterone block (cort) pretreatment. (B) Input-output summary graph with representative traces (left) and slope (right) for IPSCs recorded in BS cells after drug controls (B; P=0.4011; slope, P=0.4583; saline n=14/3, symp n=15/3, cort n=16/3). (C) Strontium-mediated aIPSC amplitudes after optogenetic stimulation for BS cells (c; P= 0.6628; saline n=15/3, symp n=13/3, cort n=13/3). (D) PPR (50ms) measurements from BS cells (D; P=0.6986; saline n=14/3, symp n=15/3, cort n=16/3). (E,I) A Cre-dependent ChIEF AVV was injected into the vSUB of PV-Cre female mice, mice received a symp (e) or cort (i) pretreatment before ARS or underwent control conditions, and optogenetically evoked IPSCs from PV interneurons were recorded from BS cells 24-hr later. (f,j) Input-output summary graph with representative traces (left) and slope (right) for IPSCs in BS cells in symp (E; P=0.1973; slope, P=0.1960; control n=13/3, symp=17/4) or cort (i; 0.213 pA, P=0.9891; 0.518 pA, P=0.2942; 1.050 pA, **P=0.0044; 1.640 pA, *P=0.0320; slope, *P=0.0419; control n=13/3, cort n=16/3) studies. (G,K) Strontium-mediated aIPSC amplitudes from BS cells after symp (G; P=0.9042; control n=11/3, stress n=15/4) or cort (K; **P=0.0018, control n=13/3, cort n=13/3) studies. (H,L) PPR (50ms) measurements in BS cells in symp (H; P=0.4673, control n=13/3, symp n=17/3) or cort (L; P=0.3180, control n=13/3, cort n=16/3) studies. Numbers in the legend represent the numbers of cells/animals. Statistical significance was determined by a 1-way ANOVA, 2-way ANOVA, or unpaired t-test. See also Figures S9.