Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Apr 15;267(2):325–330. doi: 10.1042/bj2670325

Protein synthesis in liver and extra-hepatic tissues after partial hepatectomy.

V R Preedy 1, L Paska 1, P H Sugden 1, P S Schofield 1, M C Sugden 1
PMCID: PMC1131290  PMID: 2334395

Abstract

Effects of partial hepatectomy on protein synthesis were defined in liver and extra-hepatic tissues of the mature rat. Studies were performed at 24 h and 48 h after surgery in the absence of the dietary input. Protein accretion in the regenerating liver preceded mitosis, but was accompanied by increases in RNA content and fractional rates of protein synthesis (ks). A positive relationship existed between protein-synthetic capacity and ks over the period of study. Increases in ks also bore a positive relationship with increases in translational efficiency. Extra-hepatic tissues showing decreased rates of protein synthesis after liver resection included kidney, striated muscles and brain. Effects were observed mainly at 24 h after surgery and resulted from decreased translational efficiency. Partial hepatectomy increased ks in diaphragm and tibia at both 24 h and 48 h after surgery. In diaphragm, there was net protein accretion, and, as in liver, increases in ks were due to increases in both protein-synthetic capacity and efficiency.

Full text

PDF
325

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown J. G., van Bueren J., Millward D. J. The effect of tri-iodothyronine administration on protein synthesis in the diabetic rat. Biochem J. 1983 Aug 15;214(2):637–640. doi: 10.1042/bj2140637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garlick P. J., Fern M., Preedy V. R. The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats. Biochem J. 1983 Mar 15;210(3):669–676. doi: 10.1042/bj2100669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garlick P. J., McNurlan M. A., Preedy V. R. A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine. Biochem J. 1980 Nov 15;192(2):719–723. doi: 10.1042/bj1920719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hawkins R. A., Williamson D. H., Krebs H. A. Ketone-body utilization by adult and suckling rat brain in vivo. Biochem J. 1971 Mar;122(1):13–18. doi: 10.1042/bj1220013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Issad T., Pénicaud L., Ferré P., Kandé J., Baudon M. A., Girard J. Effects of fasting on tissue glucose utilization in conscious resting rats. Major glucose-sparing effect in working muscles. Biochem J. 1987 Aug 15;246(1):241–244. doi: 10.1042/bj2460241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Katz N. Correlation between rates and enzyme levels of increased gluconeogenesis in rat liver and kidney after partial hepatectomy. Eur J Biochem. 1979 Aug 1;98(2):535–542. doi: 10.1111/j.1432-1033.1979.tb13214.x. [DOI] [PubMed] [Google Scholar]
  8. Mortimore G. E., Ward W. F. Behavior of the lysosomal system during organ perfusion. An inquiry into the mechanism of hepatic proteolysis. Front Biol. 1976;45:157–184. [PubMed] [Google Scholar]
  9. Preedy V. R., Paska L., Sugden P. H., Schofield P. S., Sugden M. C. Rat liver protein synthesis after partial hepatectomy. Biochem Soc Trans. 1989 Dec;17(6):1047–1047. doi: 10.1042/bst0171047. [DOI] [PubMed] [Google Scholar]
  10. Preedy V. R., Paska L., Sugden P. H., Schofield P. S., Sugden M. C. The effects of surgical stress and short-term fasting on protein synthesis in vivo in diverse tissues of the mature rat. Biochem J. 1988 Feb 15;250(1):179–188. doi: 10.1042/bj2500179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Preedy V. R., Smith D. M., Kearney N. F., Sugden P. H. Rates of protein turnover in vivo and in vitro in ventricular muscle of hearts from fed and starved rats. Biochem J. 1984 Sep 1;222(2):395–400. doi: 10.1042/bj2220395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schofield P. S., McLees D. J., Myles D. D., Sugden M. C. Ketone-body metabolism after partial hepatectomy in the rat. Biochem J. 1985 Oct 1;231(1):225–228. doi: 10.1042/bj2310225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scornik O. A., Botbol V. Role of changes in protein degradation in the growth of regenerating livers. J Biol Chem. 1976 May 25;251(10):2891–2897. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES