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. 1990 Apr 15;267(2):549–551. doi: 10.1042/bj2670549

Calcium activates glucose-6-phosphatase in intact rat hepatic microsomes.

I D Waddell 1, L Gibb 1, A Burchell 1
PMCID: PMC1131326  PMID: 2159288

Abstract

The effects of Ca2+ on the microsomal glucose-6-phosphatase activity were investigated. Evidence is provided that increases by Ca2+ in both the pyrophosphatase and the glucose-6-phosphate-hydrolysing activities are due to an increase in microsomal transport capacity of T2, the phosphate/pyrophosphate-transport protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arion W. J., Lange A. J., Walls H. E., Ballas L. M. Evidence for the participation of independent translocation for phosphate and glucose 6-phosphate in the microsomal glucose-6-phosphatase system. Interactions of the system with orthophosphate, inorganic pyrophosphate, and carbamyl phosphate. J Biol Chem. 1980 Nov 10;255(21):10396–10406. [PubMed] [Google Scholar]
  2. BEAUFAY H., DE DUVE C. Le système hexose-phosphatasique. IV. Spécificité de la glucose-6-phosphatase. Bull Soc Chim Biol (Paris) 1954;36(11-12):1525–1537. [PubMed] [Google Scholar]
  3. Burchell A., Bell J. E., Busuttil A., Hume R. Hepatic microsomal glucose-6-phosphatase system and sudden infant death syndrome. Lancet. 1989 Aug 5;2(8658):291–294. doi: 10.1016/s0140-6736(89)90484-4. [DOI] [PubMed] [Google Scholar]
  4. Burchell A., Burchell B., Monaco M., Walls H. E., Arion W. J. Stabilization of glucose-6-phosphatase activity by a 21 000-dalton hepatic microsomal protein. Biochem J. 1985 Sep 1;230(2):489–495. doi: 10.1042/bj2300489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burchell A., Cain D. I. Rat hepatic microsomal glucose-6-phosphatase protein levels are increased in streptozotocin-induced diabetes. Diabetologia. 1985 Nov;28(11):852–856. doi: 10.1007/BF00291077. [DOI] [PubMed] [Google Scholar]
  6. Burchell A., Hume R., Burchell B. A new microtechnique for the analysis of the human hepatic microsomal glucose-6-phosphatase system. Clin Chim Acta. 1988 Apr 15;173(2):183–191. doi: 10.1016/0009-8981(88)90256-2. [DOI] [PubMed] [Google Scholar]
  7. Burchell A., Jung R. T., Lang C. C., Bennet W., Shepherd A. N. Diagnosis of type 1a and type 1c glycogen storage diseases in adults. Lancet. 1987 May 9;1(8541):1059–1062. doi: 10.1016/s0140-6736(87)90484-3. [DOI] [PubMed] [Google Scholar]
  8. Burchell A., Waddell I. D., Stewart L., Hume R. Perinatal diagnosis of type 1c glycogen storage disease. J Inherit Metab Dis. 1989;12 (Suppl 2):315–317. doi: 10.1007/BF03335409. [DOI] [PubMed] [Google Scholar]
  9. CORI G. T., CORI C. F. Glucose-6-phosphatase of the liver in glycogen storage disease. J Biol Chem. 1952 Dec;199(2):661–667. [PubMed] [Google Scholar]
  10. Countaway J. L., Waddell I. D., Burchell A., Arion W. J. The phosphohydrolase component of the hepatic microsomal glucose-6-phosphatase system is a 36.5-kilodalton polypeptide. J Biol Chem. 1988 Feb 25;263(6):2673–2678. [PubMed] [Google Scholar]
  11. Davidson A. M., Halestrap A. P. Liver mitochondrial pyrophosphate concentration is increased by Ca2+ and regulates the intramitochondrial volume and adenine nucleotide content. Biochem J. 1987 Sep 15;246(3):715–723. doi: 10.1042/bj2460715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kamath S. A., Kummerow F. A., Narayan K. Ananth. A simple procedure for the isolation of rat liver microsomes. FEBS Lett. 1971 Sep 15;17(1):90–92. doi: 10.1016/0014-5793(71)80571-9. [DOI] [PubMed] [Google Scholar]
  13. Lange A. J., Arion W. J., Beaudet A. L. Type Ib glycogen storage disease is caused by a defect in the glucose-6-phosphate translocase of the microsomal glucose-6-phosphatase system. J Biol Chem. 1980 Sep 25;255(18):8381–8384. [PubMed] [Google Scholar]
  14. Mine T., Kojima I., Ogata E. Calcium rather than cyclic AMP is an intracellular messenger of parathyroid hormone action on glycogen metabolism in isolated rat hepatocytes. Biochem J. 1989 Mar 15;258(3):889–894. doi: 10.1042/bj2580889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. NORDLIE R. C., ARION W. J. LIVER MICROSOMAL GLUCOSE 6-PHOSPHATASE, INORGANIC PYROPHOSPHATASE, AND PYROPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE. 3. ASSOCIATED NUCLEOSIDE TRIPHOSPHATE- AND NUCLEOSIDE DIPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE ACTIVITIES. J Biol Chem. 1965 May;240:2155–2164. [PubMed] [Google Scholar]
  16. Narisawa K., Igarashi Y., Otomo H., Tada K. A new variant of glycogen storage disease type I probably due to a defect in the glucose-6-phosphate transport system. Biochem Biophys Res Commun. 1978 Aug 29;83(4):1360–1364. doi: 10.1016/0006-291x(78)91371-2. [DOI] [PubMed] [Google Scholar]
  17. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  18. SAMPSON S. R., KARLER R. CALCIUM-BINDING PROPERTIES OF SKELETAL MUSCLE MICROSOMES. J Cell Physiol. 1963 Dec;62:303–309. doi: 10.1002/jcp.1030620310. [DOI] [PubMed] [Google Scholar]
  19. SWANSON M. A. Phosphatases of liver. I. Glucose-6-phosphatase. J Biol Chem. 1950 Jun;184(2):647–659. [PubMed] [Google Scholar]
  20. Scott I. D., Akerman K. E., Nicholls D. G. Calcium-ion transport by intact synaptosomes. Intrasynaptosomal compartmentation and the role of the mitochondrial membrane potential. Biochem J. 1980 Dec 15;192(3):873–880. doi: 10.1042/bj1920873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Waddell I. D., Hume R., Burchell A. A direct method for the diagnosis of human hepatic type 1b and type 1c glycogen-storage disease. Clin Sci (Lond) 1989 Jun;76(6):573–579. doi: 10.1042/cs0760573. [DOI] [PubMed] [Google Scholar]
  22. van de Werve G. Liver glucose-6-phosphatase activity is modulated by physiological intracellular Ca2+ concentrations. J Biol Chem. 1989 Apr 15;264(11):6033–6036. [PubMed] [Google Scholar]

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