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. 1990 May 15;268(1):1–13. doi: 10.1042/bj2680001

Assembly and secretion of hepatic very-low-density lipoprotein.

G F Gibbons 1
PMCID: PMC1131384  PMID: 2188646

Abstract

In contrast to water-soluble fuels such as glucose or ketone bodies, the use of lipids as an energy source for tissues has required the development of complex structures for their transport through the aqueous plasma. In the case of endogenously synthesized triacylglycerol this is achieved by the assembly and secretion of hepatic VLDL which provides the necessary stability in an aqueous medium. An essential component of this assembly process is apo B. Dietary changes which require an increase in hepatic VLDL secretion appear to be accompanied by increases in the availability of functional apo B. Interesting questions relate to: (a) the intracellular site(s) of triacylglycerol association with apo B, and (b) the mechanism(s) by which the availability of functional apo B at this site responds to metabolic and hormonal signals which reflect dietary status and, thus, the need to secrete triacylglycerol. As regards the latter, although in some cases changes in apo B synthesis occur in response to VLDL secretion hepatic apo B mRNA levels appear to be quite stable in vitro. Intracellular switching of apo B between the secretory and degradative pathways may be important in controlling VLDL assembly and post-translational modifications of the apoprotein may also play a role by influencing its ability to bind to triacylglycerol. Transport is not the only problem associated with the utilization of a concentrated energy source such as triacylglycerol and the complex problems of waste product disposal and recycling have to be dealt with. In the case of triacylglycerol, potentially toxic waste products include atherogenic remnants and LDL. The overall problem, then, in the long-term, involves the development of a 'safe' means of utilizing triacylglycerol and this requirement accounts for much of the complexity of plasma lipoprotein metabolism. In this area, the rat could teach the human a few tricks. One of these appears to be the utilization of hepatic apo B48 rather than apo B100 for VLDL assembly in response to increases in the extrahepatic utilization of hepatically synthesized triacylglycerol. Under these conditions, the remnants of hepatic triacylglycerol utilization by peripheral tissues are cleared from the plasma much more readily via a process which seems to involve the cycling of more triacylglycerol back to the liver than that which occurs in humans. The means by which this is achieved, though, are obscure and may involve a chylomicron remnant receptor, the nature of which, itself, remains controversial.(ABSTRACT TRUNCATED AT 400 WORDS)

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott W. G., Lillioja S., Young A. A., Zawadzki J. K., Yki-Järvinen H., Christin L., Howard B. V. Relationships between plasma lipoprotein concentrations and insulin action in an obese hyperinsulinemic population. Diabetes. 1987 Aug;36(8):897–904. doi: 10.2337/diab.36.8.897. [DOI] [PubMed] [Google Scholar]
  2. Agius L., Blackshear P. J., Williamson D. H. Rates of triacylglycerol entry into the circulation in the lactating rat. Biochem J. 1981 May 15;196(2):637–640. doi: 10.1042/bj1960637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alexander C. A., Hamilton R. L., Havel R. J. Subcellular localization of B apoprotein of plasma lipoproteins in rat liver. J Cell Biol. 1976 May;69(2):241–263. doi: 10.1083/jcb.69.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Arbeeny C. M., Edelstein D., Freedman S. R., Eder H. A. Serum lipoproteins of diabetic rats fed a high cholesterol diet. Diabetes. 1980 Oct;29(10):774–777. doi: 10.2337/diacare.20.10.774. [DOI] [PubMed] [Google Scholar]
  5. Azain M. J., Fukuda N., Chao F. F., Yamamoto M., Ontko J. A. Contributions of fatty acid and sterol synthesis to triglyceride and cholesterol secretion by the perfused rat liver in genetic hyperlipemia and obesity. J Biol Chem. 1985 Jan 10;260(1):174–181. [PubMed] [Google Scholar]
  6. BAKER N., SCHOTZ M. C. USE OF MULTICOMPARTMENTAL MODELS TO MEASURE RATES OF TRIGLYCERIDE METABOLISM IN RATS. J Lipid Res. 1964 Apr;5:188–197. [PubMed] [Google Scholar]
  7. Baltzell J. K., Berdanier C. D. Effect on the interaction of dietary carbohydrate and fat on the responses of rats to starvation-refeeding. J Nutr. 1985 Jan;115(1):104–110. doi: 10.1093/jn/115.1.104. [DOI] [PubMed] [Google Scholar]
  8. Bamberger M. J., Lane M. D. Assembly of very low density lipoprotein in the hepatocyte. Differential transport of apoproteins through the secretory pathway. J Biol Chem. 1988 Aug 25;263(24):11868–11878. [PubMed] [Google Scholar]
  9. Baraona E., Lieber C. S. Efcts of chronic ethanol feeding on serum lipoprotein metabolism in the rat. J Clin Invest. 1970 Apr;49(4):769–778. doi: 10.1172/JCI106290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Bartlett S. M., Gibbons G. F. Short- and longer-term regulation of very-low-density lipoprotein secretion by insulin, dexamethasone and lipogenic substrates in cultured hepatocytes. A biphasic effect of insulin. Biochem J. 1988 Jan 1;249(1):37–43. doi: 10.1042/bj2490037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Basso L. V., Havel R. J. Hepatic metabolism of free fatty acids in normal and diabetic dogs. J Clin Invest. 1970 Mar;49(3):537–547. doi: 10.1172/JCI106264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Berry E. M., Ziv E., Bar-On H. Lipoprotein secretion by isolated perfused livers from streptozotocin-diabetic rats. Diabetologia. 1981 Oct;21(4):402–408. doi: 10.1007/BF00252689. [DOI] [PubMed] [Google Scholar]
  13. Boogaerts J. R., Malone-McNeal M., Archambault-Schexnayder J., Davis R. A. Dietary carbohydrate induces lipogenesis and very-low-density lipoprotein synthesis. Am J Physiol. 1984 Jan;246(1 Pt 1):E77–E83. doi: 10.1152/ajpendo.1984.246.1.E77. [DOI] [PubMed] [Google Scholar]
  14. Borchardt R. A., Davis R. A. Intrahepatic assembly of very low density lipoproteins. Rate of transport out of the endoplasmic reticulum determines rate of secretion. J Biol Chem. 1987 Dec 5;262(34):16394–16402. [PubMed] [Google Scholar]
  15. Boström K., Borén J., Wettesten M., Sjöberg A., Bondjers G., Wiklund O., Carlsson P., Olofsson S. O. Studies on the assembly of apo B-100-containing lipoproteins in HepG2 cells. J Biol Chem. 1988 Mar 25;263(9):4434–4442. [PubMed] [Google Scholar]
  16. Boström K., Lauer S. J., Poksay K. S., Garcia Z., Taylor J. M., Innerarity T. L. Apolipoprotein B48 RNA editing in chimeric apolipoprotein EB mRNA. J Biol Chem. 1989 Sep 15;264(26):15701–15708. [PubMed] [Google Scholar]
  17. Boström K., Wettesten M., Borén J., Bondjers G., Wiklund O., Olofsson S. O. Pulse-chase studies of the synthesis and intracellular transport of apolipoprotein B-100 in Hep G2 cells. J Biol Chem. 1986 Oct 15;261(29):13800–13806. [PubMed] [Google Scholar]
  18. Brindle N. P., Ontko J. A. Alpha-adrenergic suppression of very-low-density-lipoprotein triacylglycerol secretion by isolated rat hepatocytes. Biochem J. 1988 Mar 1;250(2):363–368. doi: 10.1042/bj2500363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Brindley D. N. Regulation of hepatic triacylglycerol synthesis and lipoprotein metabolism by glucocorticoids. Clin Sci (Lond) 1981 Aug;61(2):129–133. doi: 10.1042/cs0610129. [DOI] [PubMed] [Google Scholar]
  20. Cairns S. R., Peters T. J. Isolation of micro- and macro-droplet fractions from needle biopsy specimens of human liver and determination of the subcellular distribution of the accumulating liver lipids in alcoholic fatty liver. Clin Sci (Lond) 1984 Sep;67(3):337–345. doi: 10.1042/cs0670337. [DOI] [PubMed] [Google Scholar]
  21. Chait A., Brunzell J. D., Johnson D. G., Benson J. W., Werner P., Palmer J. P., Albers J. J., Ensinck J. W., Bierman E. L. Reduction of plasma triglyceride concentration by acute stress in man. Metabolism. 1979 May;28(5):553–561. doi: 10.1016/0026-0495(79)90197-5. [DOI] [PubMed] [Google Scholar]
  22. Chao F. F., Stiers D. L., Ontko J. A. Hepatocellular triglyceride synthesis and transfer to lipid droplets and nascent very low density lipoproteins. J Lipid Res. 1986 Nov;27(11):1174–1181. [PubMed] [Google Scholar]
  23. Chen S. H., Habib G., Yang C. Y., Gu Z. W., Lee B. R., Weng S. A., Silberman S. R., Cai S. J., Deslypere J. P., Rosseneu M. Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon. Science. 1987 Oct 16;238(4825):363–366. doi: 10.1126/science.3659919. [DOI] [PubMed] [Google Scholar]
  24. Chen S. H., Yang C. Y., Chen P. F., Setzer D., Tanimura M., Li W. H., Gotto A. M., Jr, Chan L. The complete cDNA and amino acid sequence of human apolipoprotein B-100. J Biol Chem. 1986 Oct 5;261(28):12918–12921. [PubMed] [Google Scholar]
  25. Cole T. G., Wilcox H. G., Heimberg M. Effects of adrenalectomy and dexamethasone on hepatic lipid metabolism. J Lipid Res. 1982 Jan;23(1):81–91. [PubMed] [Google Scholar]
  26. Coleman R. A., Haynes E. B., Sand T. M., Davis R. A. Developmental coordinate expression of triacylglycerol and small molecular weight apoB synthesis and secretion by rat hepatocytes. J Lipid Res. 1988 Jan;29(1):33–42. [PubMed] [Google Scholar]
  27. Crouse J. R., Grundy S. M. Effects of alcohol on plasma lipoproteins and cholesterol and triglyceride metabolism in man. J Lipid Res. 1984 May;25(5):486–496. [PubMed] [Google Scholar]
  28. Daggy B., Arost C., Bensadoun A. Dietary fish oil decreases VLDL production rates. Biochim Biophys Acta. 1987 Aug 15;920(3):293–300. doi: 10.1016/0005-2760(87)90107-x. [DOI] [PubMed] [Google Scholar]
  29. Dashti N., Wolfbauer G. Secretion of lipids, apolipoproteins, and lipoproteins by human hepatoma cell line, HepG2: effects of oleic acid and insulin. J Lipid Res. 1987 Apr;28(4):423–436. [PubMed] [Google Scholar]
  30. Davidson N. O., Carlos R. C., Drewek M. J., Parmer T. G. Apolipoprotein gene expression in the rat is regulated in a tissue-specific manner by thyroid hormone. J Lipid Res. 1988 Nov;29(11):1511–1522. [PubMed] [Google Scholar]
  31. Davidson N. O., Powell L. M., Wallis S. C., Scott J. Thyroid hormone modulates the introduction of a stop codon in rat liver apolipoprotein B messenger RNA. J Biol Chem. 1988 Sep 25;263(27):13482–13485. [PubMed] [Google Scholar]
  32. Davies M. S., Wallis S. C., Driscoll D. M., Wynne J. K., Williams G. W., Powell L. M., Scott J. Sequence requirements for apolipoprotein B RNA editing in transfected rat hepatoma cells. J Biol Chem. 1989 Aug 15;264(23):13395–13398. [PubMed] [Google Scholar]
  33. Davis R. A., Boogaerts J. R., Borchardt R. A., Malone-McNeal M., Archambault-Schexnayder J. Intrahepatic assembly of very low density lipoproteins. Varied synthetic response of individual apolipoproteins to fasting. J Biol Chem. 1985 Nov 15;260(26):14137–14144. [PubMed] [Google Scholar]
  34. Davis R. A., Boogaerts J. R. Intrahepatic assembly of very low density lipoproteins. Effect of fatty acids on triacylglycerol and apolipoprotein synthesis. J Biol Chem. 1982 Sep 25;257(18):10908–10913. [PubMed] [Google Scholar]
  35. Davis R. A., Borchardt R. A. Phosphorylation-dephosphorylation of apolipoprotein B. Methods Enzymol. 1986;129:536–542. doi: 10.1016/0076-6879(86)29090-4. [DOI] [PubMed] [Google Scholar]
  36. Davis R. A., Clinton G. M., Borchardt R. A., Malone-McNeal M., Tan T., Lattier G. R. Intrahepatic assembly of very low density lipoproteins. Phosphorylation of small molecular weight apolipoprotein B. J Biol Chem. 1984 Mar 25;259(6):3383–3386. [PubMed] [Google Scholar]
  37. Davis R. A., Prewett A. B., Chan D. C., Thompson J. J., Borchardt R. A., Gallaher W. R. Intrahepatic assembly of very low density lipoproteins: immunologic characterization of apolipoprotein B in lipoproteins and hepatic membrane fractions and its intracellular distribution. J Lipid Res. 1989 Aug;30(8):1185–1196. [PubMed] [Google Scholar]
  38. Debeer L. J., Beynen A. C., Mannaerts G. P., Geelen M. J. Lipolysis of hepatic triacylglycerol stores. FEBS Lett. 1982 Apr 19;140(2):159–164. doi: 10.1016/0014-5793(82)80884-3. [DOI] [PubMed] [Google Scholar]
  39. Demmer L. A., Levin M. S., Elovson J., Reuben M. A., Lusis A. J., Gordon J. I. Tissue-specific expression and developmental regulation of the rat apolipoprotein B gene. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8102–8106. doi: 10.1073/pnas.83.21.8102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Dich J., Bro B., Grunnet N., Jensen F., Kondrup J. Accumulation of triacylglycerol in cultured rat hepatocytes is increased by ethanol and by insulin and dexamethasone. Biochem J. 1983 Jun 15;212(3):617–623. doi: 10.1042/bj2120617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Dolphin P. J., Rubinstein D. Glycosylation of apoproteins of rat very low density lipoproteins during transit through the hepatic Golgi apparatus. Can J Biochem. 1977 Jan;55(1):83–90. doi: 10.1139/o77-014. [DOI] [PubMed] [Google Scholar]
  42. Duerden J. M., Bartlett S. M., Gibbons G. F. Long-term maintenance of high rates of very-low-density-lipoprotein secretion in hepatocyte cultures. A model for studying the direct effects of insulin and insulin deficiency in vitro. Biochem J. 1989 Nov 1;263(3):937–943. doi: 10.1042/bj2630937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Duerden J. M., Bartlett S. M., Gibbons G. F. Regulation of very-low-density-lipoprotein lipid secretion in hepatocyte cultures derived from diabetic animals. Biochem J. 1989 Aug 15;262(1):313–319. doi: 10.1042/bj2620313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Duerden J. M., Gibbons G. F. Secretion and storage of newly synthesized hepatic triacylglycerol fatty acids in vivo in different nutritional states and in diabetes. Biochem J. 1988 Nov 1;255(3):929–935. doi: 10.1042/bj2550929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Dunphy W. G., Rothman J. E. Compartmental organization of the Golgi stack. Cell. 1985 Aug;42(1):13–21. doi: 10.1016/s0092-8674(85)80097-0. [DOI] [PubMed] [Google Scholar]
  46. Durrington P. N., Newton R. S., Weinstein D. B., Steinberg D. Effects of insulin and glucose on very low density lipoprotein triglyceride secretion by cultured rat hepatocytes. J Clin Invest. 1982 Jul;70(1):63–73. doi: 10.1172/JCI110604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Edwards P. A., Lemongello D., Fogelman A. M. The effect of glucagon, norepinephrine, and dibutyryl cyclic AMP on cholesterol efflux and on the activity of 3-hydroxy-3-methylglutaryl CoA reductase in rat hepatocytes. J Lipid Res. 1979 Jan;20(1):2–7. [PubMed] [Google Scholar]
  48. Egusa G., Beltz W. F., Grundy S. M., Howard B. V. Influence of obesity on the metabolism of apolipoprotein B in humans. J Clin Invest. 1985 Aug;76(2):596–603. doi: 10.1172/JCI112011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Ehrenreich J. H., Bergeron J. J., Siekevitz P., Palade G. E. Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization. J Cell Biol. 1973 Oct;59(1):45–72. doi: 10.1083/jcb.59.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Elovson J., Huang Y. O., Baker N., Kannan R. Apolipoprotein B is structurally and metabolically heterogeneous in the rat. Proc Natl Acad Sci U S A. 1981 Jan;78(1):157–161. doi: 10.1073/pnas.78.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Engelken S. F., Eaton R. P. The effects of altered thyroid status on lipid metabolism in the genetic hyperlipemic Zucker rat. Atherosclerosis. 1981 Jan-Feb;38(1-2):177–188. doi: 10.1016/0021-9150(81)90114-3. [DOI] [PubMed] [Google Scholar]
  52. Engelken S. F., Eaton R. P. Thyroid hormone-induced dissociation between plasma triglyceride and cholesterol regulation in the rat. Endocrinology. 1980 Jul;107(1):208–214. doi: 10.1210/endo-107-1-208. [DOI] [PubMed] [Google Scholar]
  53. FARQUHAR J. W., GROSS R. C., WAGNER R. M., REAVEN G. M. VALIDATION OF AN INCOMPLETELY COUPLED TWO-COMPARTMENT NONRECYCLING CATENARY MODEL FOR TURNOVER OF LIVER AND PLASMA TRIGLYCERIDE IN MAN. J Lipid Res. 1965 Jan;6:119–134. [PubMed] [Google Scholar]
  54. Farquhar M. G. Progress in unraveling pathways of Golgi traffic. Annu Rev Cell Biol. 1985;1:447–488. doi: 10.1146/annurev.cb.01.110185.002311. [DOI] [PubMed] [Google Scholar]
  55. Feingold K. R., Lear S. R., Moser A. H. De novo cholesterol synthesis in three different animal models of diabetes. Diabetologia. 1984 Mar;26(3):234–239. doi: 10.1007/BF00252414. [DOI] [PubMed] [Google Scholar]
  56. Fernando-Warnakulasuriya G. J., Eckerson M. L., Clark W. A., Wells M. A. Lipoprotein metabolism in the suckling rat: characterization of plasma and lymphatic lipoproteins. J Lipid Res. 1983 Dec;24(12):1626–1638. [PubMed] [Google Scholar]
  57. Francone O. L., Kalopissis A. D., Griffaton G. Contribution of cytoplasmic storage triacylglycerol to VLDL-triacylglycerol in isolated rat hepatocytes. Biochim Biophys Acta. 1989 Mar 14;1002(1):28–36. doi: 10.1016/0005-2760(89)90060-x. [DOI] [PubMed] [Google Scholar]
  58. Frayn K. N. Hormonal control of metabolism in trauma and sepsis. Clin Endocrinol (Oxf) 1986 May;24(5):577–599. doi: 10.1111/j.1365-2265.1986.tb03288.x. [DOI] [PubMed] [Google Scholar]
  59. Frost S. C., Clark W. A., Wells M. A. Studies on fat digestion, absorption, and transport in the suckling rat. IV. In vivo rates of triacylglycerol secretion by intestine and liver. J Lipid Res. 1983 Jul;24(7):899–903. [PubMed] [Google Scholar]
  60. Fukuda N., Azain M. J., Ontko J. A. Altered hepatic metabolism of free fatty acids underlying hypersecretion of very low density lipoproteins in the genetically obese Zucker rats. J Biol Chem. 1982 Dec 10;257(23):14066–14072. [PubMed] [Google Scholar]
  61. Geelen M. J., Harris R. A., Beynen A. C., McCune S. A. Short-term hormonal control of hepatic lipogenesis. Diabetes. 1980 Dec;29(12):1006–1022. doi: 10.2337/diab.29.12.1006. [DOI] [PubMed] [Google Scholar]
  62. Getz G. S., Soltys P. A., Carey K. D., McGill H. C., Jr, Hay R. Nutrition and biogenesis of plasma lipoproteins in nonhuman primates. Am Heart J. 1987 Feb;113(2 Pt 2):440–445. doi: 10.1016/0002-8703(87)90611-9. [DOI] [PubMed] [Google Scholar]
  63. Gibbons G. F. Hyperlipidaemia of diabetes. Clin Sci (Lond) 1986 Nov;71(5):477–486. doi: 10.1042/cs0710477. [DOI] [PubMed] [Google Scholar]
  64. Gibbons G. F. Insulin, diabetes and hepatic very-low-density lipoprotein metabolism. Biochem Soc Trans. 1989 Feb;17(1):49–51. doi: 10.1042/bst0170049. [DOI] [PubMed] [Google Scholar]
  65. Gibbons G. F., Pullinger C. R., Björnsson O. G. Changes in the sensitivity of lipogenesis in rat hepatocytes to hormones and precursors over the diurnal cycle and during longer-term starvation of donor animals. J Lipid Res. 1984 Dec 1;25(12):1358–1367. [PubMed] [Google Scholar]
  66. Gibbons G. F., Pullinger C. R. Diurnal variations in the effects of an unsaturated-fat-containing diet on fatty acid and cholesterol synthesis in rat hepatocytes. Biochem J. 1986 Nov 1;239(3):617–623. doi: 10.1042/bj2390617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Gibbons G. F., Pullinger C. R. Regulation of hepatic very-low-density lipoprotein secretion in rats fed on a diet high in unsaturated fat. Biochem J. 1987 Apr 15;243(2):487–492. doi: 10.1042/bj2430487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Glaumann H., Bergstrand A., Ericsson J. L. Studies on the synthesis and intracellular transport of lipoprotein particles in rat liver. J Cell Biol. 1975 Feb;64(2):356–377. doi: 10.1083/jcb.64.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. Green R. P., Birkenmeier E. H., Beamer W. G., Maltais L. J., Gordon J. I. The hypothyroid (hyt/hyt) mouse: a model system for studying the effects of thyroid hormone on developmental changes in gene expression. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5592–5596. doi: 10.1073/pnas.85.15.5592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  70. Greenfield M., Kolterman O., Olefsky J., Reaven G. M. Mechanism of hypertriglyceridaemia in diabetic patients with fasting hyperglycaemia. Diabetologia. 1980 Jun;18(6):441–446. doi: 10.1007/BF00261698. [DOI] [PubMed] [Google Scholar]
  71. Groot P. H., de Boer B. C., Haddeman E., Houtsmuller U. M., Hülsmann W. C. Effect of dietary fat composition on the metabolism of triacylglycerol-rich plasma lipoproteins in the postprandial phase in meal-fed rats. J Lipid Res. 1988 May;29(5):541–551. [PubMed] [Google Scholar]
  72. Grunnet N., Jensen F., Kondrup J., Dich J. Effect of ethanol on fatty acid metabolism in cultured hepatocytes: dependency on incubation time and fatty acid concentration. Alcohol. 1985 Jan-Feb;2(1):157–161. doi: 10.1016/0741-8329(85)90035-7. [DOI] [PubMed] [Google Scholar]
  73. Hahn P., Girard J., Assan R., Frohlich J., Kervran A. Control of blood cholesterol levels in suckling and weanling rats. J Nutr. 1977 Nov;107(11):2062–2066. doi: 10.1093/jn/107.11.2062. [DOI] [PubMed] [Google Scholar]
  74. Hardman D. A., Kane J. P. Isolation and characterization of apolipoprotein B-48. Methods Enzymol. 1986;128:262–272. doi: 10.1016/0076-6879(86)28072-6. [DOI] [PubMed] [Google Scholar]
  75. Harris W. S., Connor W. E., Alam N., Illingworth D. R. Reduction of postprandial triglyceridemia in humans by dietary n-3 fatty acids. J Lipid Res. 1988 Nov;29(11):1451–1460. [PubMed] [Google Scholar]
  76. Harris W. S. Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review. J Lipid Res. 1989 Jun;30(6):785–807. [PubMed] [Google Scholar]
  77. Havel R. J., Kane J. P., Balasse E. O., Segel N., Basso L. V. Splanchnic metabolism of free fatty acids and production of triglycerides of very low density lipoproteins in normotriglyceridemic and hypertriglyceridemic humans. J Clin Invest. 1970 Nov;49(11):2017–2035. doi: 10.1172/JCI106422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  78. Havel R. J. Role of the liver in lipoprotein catabolism. Methods Enzymol. 1986;129:591–612. doi: 10.1016/0076-6879(86)29093-x. [DOI] [PubMed] [Google Scholar]
  79. Hay R., Fleming R., O'Connell W., Kirschner J., Oppliger W. Apolipoproteins of the orotic acid fatty liver: implications for the biogenesis of plasma lipoproteins. J Lipid Res. 1988 Aug;29(8):981–995. [PubMed] [Google Scholar]
  80. Heimberg M., Olubadewo J. O., Wilcox H. G. Plasma lipoproteins and regulation of hepatic metabolism of fatty acids in altered thyroid states. Endocr Rev. 1985 Fall;6(4):590–607. doi: 10.1210/edrv-6-4-590. [DOI] [PubMed] [Google Scholar]
  81. Higgins J. A., Fieldsend J. K. Phosphatidylcholine synthesis for incorporation into membranes or for secretion as plasma lipoproteins by Golgi membranes of rat liver. J Lipid Res. 1987 Mar;28(3):268–278. [PubMed] [Google Scholar]
  82. Higgins J. A., Hutson J. L. The roles of Golgi and endoplasmic reticulum in the synthesis and assembly of lipoprotein lipids in rat hepatocytes. J Lipid Res. 1984 Dec 1;25(12):1295–1305. [PubMed] [Google Scholar]
  83. Hoeg J. M., Meng M. S., Ronan R., Demosky S. J., Jr, Fairwell T., Brewer H. B., Jr Apolipoprotein B synthesized by Hep G2 cells undergoes fatty acid acylation. J Lipid Res. 1988 Sep;29(9):1215–1220. [PubMed] [Google Scholar]
  84. Hospattankar A. V., Higuchi K., Law S. W., Meglin N., Brewer H. B., Jr Identification of a novel in-frame translational stop codon in human intestine apoB mRNA. Biochem Biophys Res Commun. 1987 Oct 14;148(1):279–285. doi: 10.1016/0006-291x(87)91107-7. [DOI] [PubMed] [Google Scholar]
  85. Howard B. V., Abbott W. G., Egusa G., Taskinen M. R. Coordination of very low-density lipoprotein triglyceride and apolipoprotein B metabolism in humans: effects of obesity and non-insulin-dependent diabetes mellitus. Am Heart J. 1987 Feb;113(2 Pt 2):522–526. doi: 10.1016/0002-8703(87)90625-9. [DOI] [PubMed] [Google Scholar]
  86. Howard B. V. Lipoprotein metabolism in diabetes mellitus. J Lipid Res. 1987 Jun;28(6):613–628. [PubMed] [Google Scholar]
  87. Howell K. E., Palade G. E. Heterogeneity of lipoprotein particles in hepatic Golgi fractions. J Cell Biol. 1982 Mar;92(3):833–845. doi: 10.1083/jcb.92.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  88. Huang G., Lee D. M., Singh S. Identification of the thiol ester linked lipids in apolipoprotein B. Biochemistry. 1988 Mar 8;27(5):1395–1400. doi: 10.1021/bi00405a001. [DOI] [PubMed] [Google Scholar]
  89. Huang M. T., Williams M. A. Essential fatty acid deficiency and plasma triglyceride turnover in rats. Am J Physiol. 1980 May;238(5):E499–E505. doi: 10.1152/ajpendo.1980.238.5.E499. [DOI] [PubMed] [Google Scholar]
  90. ISSELBACHER K. J., SCHEIG R., PLOTKIN G. R., CAULFIELD J. B. CONGENITAL BETA-LIPOPROTEIN DEFICIENCY: AN HEREDITARY DISORDER INVOLVING A DEFECT IN THE ABSORPTION AND TRANSPORT OF LIPIDS. Medicine (Baltimore) 1964 May;43:347–361. [PubMed] [Google Scholar]
  91. Imaizumi K., Lu Y. F., Sugano M. Secretion of apolipoproteins in the suckling rat. Absence of low-molecular-weight apolipoprotein B of hepatic origin. Biochim Biophys Acta. 1985 Dec 4;837(3):345–348. [PubMed] [Google Scholar]
  92. Kalopissis A. D., Griglio S., Malewiak M. I., Rozen R. Effect of a high-fat diet on rat very low density lipoprotein secretion. Biochim Biophys Acta. 1980 Oct 6;620(1):111–119. doi: 10.1016/0005-2760(80)90190-3. [DOI] [PubMed] [Google Scholar]
  93. Kalopissis A. D., Griglio S., Malewiak M. I., Rozen R., Liepvre X. L. Very-low-density-lipoprotein secretion by isolated hepatocytes of fat-fed rats. Biochem J. 1981 Aug 15;198(2):373–377. doi: 10.1042/bj1980373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  94. Kane J. P. Apolipoprotein B: structural and metabolic heterogeneity. Annu Rev Physiol. 1983;45:637–650. doi: 10.1146/annurev.ph.45.030183.003225. [DOI] [PubMed] [Google Scholar]
  95. Kannan R., Learn D. B., Baker N., Elovson J. Fatty acid synthesis in vivo and hepatic contribution to whole-body lipogenic rates in obese Zucker rats. Lipids. 1980 Dec;15(12):993–998. doi: 10.1007/BF02534313. [DOI] [PubMed] [Google Scholar]
  96. Kaufman J. F., Krangel M. S., Strominger J. L. Cysteines in the transmembrane region of major histocompatibility complex antigens are fatty acylated via thioester bonds. J Biol Chem. 1984 Jun 10;259(11):7230–7238. [PubMed] [Google Scholar]
  97. Kelly R. B. Pathways of protein secretion in eukaryotes. Science. 1985 Oct 4;230(4721):25–32. doi: 10.1126/science.2994224. [DOI] [PubMed] [Google Scholar]
  98. Kesäniemi Y. A., Beltz W. F., Grundy S. M. Comparisons of metabolism of apolipoprotein B in normal subjects, obese patients, and patients with coronary heart disease. J Clin Invest. 1985 Aug;76(2):586–595. doi: 10.1172/JCI112010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  99. Kissebah A. H., Alfarsi S., Adams P. W. Integrated regulation of very low density lipoprotein triglyceride and apolipoprotein-B kinetics in man: normolipemic subjects, familial hypertriglyceridemia and familial combined hyperlipidemia. Metabolism. 1981 Sep;30(9):856–868. doi: 10.1016/0026-0495(81)90064-0. [DOI] [PubMed] [Google Scholar]
  100. Knott T. J., Pease R. J., Powell L. M., Wallis S. C., Rall S. C., Jr, Innerarity T. L., Blackhart B., Taylor W. H., Marcel Y., Milne R. Complete protein sequence and identification of structural domains of human apolipoprotein B. Nature. 1986 Oct 23;323(6090):734–738. doi: 10.1038/323734a0. [DOI] [PubMed] [Google Scholar]
  101. Kostner G. M., Karádi I. Lipoprotein alterations in diabetes mellitus. Diabetologia. 1988 Oct;31(10):717–722. doi: 10.1007/BF00274772. [DOI] [PubMed] [Google Scholar]
  102. Kotlar T. J., Borensztajn J. Oscillatory changes in muscle lipoprotein lipase activity of fed and starved rats. Am J Physiol. 1977 Oct;233(4):E316–E319. doi: 10.1152/ajpendo.1977.233.4.E316. [DOI] [PubMed] [Google Scholar]
  103. Laker M. E., Mayes P. A. Investigations into the direct effects of insulin on hepatic ketogenesis, lipoprotein secretion and pyruvate dehydrogenase activity. Biochim Biophys Acta. 1984 Sep 12;795(2):427–430. doi: 10.1016/0005-2760(84)90094-8. [DOI] [PubMed] [Google Scholar]
  104. Lamb R. G., Bow S. J., Wright T. O. Effects of chronic insulin and glucagon exposure on the biosynthesis of glycerolipids by cultured hepatocytes. J Biol Chem. 1982 Dec 25;257(24):15022–15025. [PubMed] [Google Scholar]
  105. Lingappa V. R. Intracellular traffic of newly synthesized proteins. Current understanding and future prospects. J Clin Invest. 1989 Mar;83(3):739–751. doi: 10.1172/JCI113952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  106. Lipkin E. W., Cooper C., Shipley R. A. The contribution of serum triacylglycerol to hepatic triacylglycerol turnover in the starved rat. Biochem J. 1978 May 15;172(2):205–218. doi: 10.1042/bj1720205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  107. Lodish H. F., Kong N., Snider M., Strous G. J. Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates. Nature. 1983 Jul 7;304(5921):80–83. doi: 10.1038/304080a0. [DOI] [PubMed] [Google Scholar]
  108. Magee A. I., Schlesinger M. J. Fatty acid acylation of eucaryotic cell membrane proteins. Biochim Biophys Acta. 1982 Nov 30;694(3):279–289. doi: 10.1016/0304-4157(82)90008-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  109. Mangiapane E. H., Brindley D. N. Effects of dexamethasone and insulin on the synthesis of triacylglycerols and phosphatidylcholine and the secretion of very-low-density lipoproteins and lysophosphatidylcholine by monolayer cultures of rat hepatocytes. Biochem J. 1986 Jan 1;233(1):151–160. doi: 10.1042/bj2330151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  110. Marrino P., Gavish D., Shafrir E., Eisenberg S. Diurnal variations of plasma lipids, tissue and plasma lipoprotein lipase, and VLDL secretion rates in the rat. A model for studies of VLDL metabolism. Biochim Biophys Acta. 1987 Aug 15;920(3):277–284. doi: 10.1016/0005-2760(87)90105-6. [DOI] [PubMed] [Google Scholar]
  111. Marsh J. B. Hepatic lipoprotein biosynthesis. Methods Enzymol. 1986;129:498–519. doi: 10.1016/0076-6879(86)29088-6. [DOI] [PubMed] [Google Scholar]
  112. McBurney L. J., Radomski M. W. Metabolism of serum free fatty acid and low-density lipoproteins in the cold-acclimated rat. Am J Physiol. 1969 Jul;217(1):19–23. doi: 10.1152/ajplegacy.1969.217.1.19. [DOI] [PubMed] [Google Scholar]
  113. Murthy V. K., Shipp J. C. Synthesis and accumulation of triglycerides in liver of diabetic rats. Effects of insulin treatment. Diabetes. 1979 May;28(5):472–478. doi: 10.2337/diab.28.5.472. [DOI] [PubMed] [Google Scholar]
  114. Nagata Y., Zilversmit D. B. Blockade of intestinal lipoprotein clearance in rabbits injected with Triton WR 1339-ethyl oleate. J Lipid Res. 1987 Jun;28(6):684–692. [PubMed] [Google Scholar]
  115. Nestel P. J., Connor W. E., Reardon M. F., Connor S., Wong S., Boston R. Suppression by diets rich in fish oil of very low density lipoprotein production in man. J Clin Invest. 1984 Jul;74(1):82–89. doi: 10.1172/JCI111422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  116. Nestel P. J. Triglyceride turnover in man. Effects of dietary carbohydrate. Prog Biochem Pharmacol. 1973;8:125–160. [PubMed] [Google Scholar]
  117. Nikkilä E. A., Kekki M. Plasma triglyceride transport kinetics in diabetes mellitus. Metabolism. 1973 Jan;22(1):1–22. doi: 10.1016/0026-0495(73)90024-3. [DOI] [PubMed] [Google Scholar]
  118. Nossen J. O., Rustan A. C., Barnard T., Drevon C. A. Inhibition by chloroquine of the secretion of very low density lipoproteins by cultured rat hepatocytes. Biochim Biophys Acta. 1984 Feb 17;803(1-2):11–20. doi: 10.1016/0167-4889(84)90049-1. [DOI] [PubMed] [Google Scholar]
  119. Novikoff P. M., Roheim P. S., Novikoff A. B., Edelstein D. Production and prevention of fatty liver in rats fed clofibrate and orotic acid diets containing sucrose. Lab Invest. 1974 Jun;30(6):732–750. [PubMed] [Google Scholar]
  120. Olefsky J. M., Farquhar J. W., Reaven G. M. Reappraisal of the role of insulin in hypertriglyceridemia. Am J Med. 1974 Oct;57(4):551–560. doi: 10.1016/0002-9343(74)90006-0. [DOI] [PubMed] [Google Scholar]
  121. Olofsson S. O., Bjursell G., Boström K., Carlsson P., Elovson J., Protter A. A., Reuben M. A., Bondjers G. Apolipoprotein B: structure, biosynthesis and role in the lipoprotein assembly process. Atherosclerosis. 1987 Nov;68(1-2):1–17. doi: 10.1016/0021-9150(87)90088-8. [DOI] [PubMed] [Google Scholar]
  122. Ong J. M., Kern P. A. Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue. J Clin Invest. 1989 Jul;84(1):305–311. doi: 10.1172/JCI114155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  123. Otway S., Robinson D. S. The use of a non-ionic detergent (Triton WR 1339) to determine rates of triglyceride entry into the circulation of the rat under different physiological conditions. J Physiol. 1967 May;190(2):321–332. doi: 10.1113/jphysiol.1967.sp008211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  124. Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
  125. Palmer J. F., Cooper C., Shipley R. A. Rate of release of hepatic triacylglycerol into serum in the starved rat. Biochem J. 1978 May 15;172(2):219–226. doi: 10.1042/bj1720219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  126. Patsch W., Franz S., Schonfeld G. Role of insulin in lipoprotein secretion by cultured rat hepatocytes. J Clin Invest. 1983 May;71(5):1161–1174. doi: 10.1172/JCI110865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  127. Patsch W., Gotto A. M., Jr, Patsch J. R. Effects of insulin on lipoprotein secretion in rat hepatocyte cultures. The role of the insulin receptor. J Biol Chem. 1986 Jul 25;261(21):9603–9606. [PubMed] [Google Scholar]
  128. Pfeffer S. R., Rothman J. E. Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi. Annu Rev Biochem. 1987;56:829–852. doi: 10.1146/annurev.bi.56.070187.004145. [DOI] [PubMed] [Google Scholar]
  129. Pietri A. O., Dunn F. L., Grundy S. M., Raskin P. The effect of continuous subcutaneous insulin infusion on very-low-density lipoprotein triglyceride metabolism in type I diabetes mellitus. Diabetes. 1983 Jan;32(1):75–81. doi: 10.2337/diab.32.1.75. [DOI] [PubMed] [Google Scholar]
  130. Popper D. A., Shiau Y. F., Reed M. Role of small intestine in pathogenesis of hyperlipidemia in diabetic rats. Am J Physiol. 1985 Aug;249(2 Pt 1):G161–G167. doi: 10.1152/ajpgi.1985.249.2.G161. [DOI] [PubMed] [Google Scholar]
  131. Pottenger L. A., Getz G. S. Serum lipoprotein accumulation in the livers of orotic acid-fed rats. J Lipid Res. 1971 Jul;12(4):450–459. [PubMed] [Google Scholar]
  132. Powell L. M., Wallis S. C., Pease R. J., Edwards Y. H., Knott T. J., Scott J. A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine. Cell. 1987 Sep 11;50(6):831–840. doi: 10.1016/0092-8674(87)90510-1. [DOI] [PubMed] [Google Scholar]
  133. Pullinger C. R., Gibbons G. F. Effects of hormones and pyruvate on the rates of secretion of very-low-density lipoprotein triacylglycerol and cholesterol by rat hepatocytes. Biochim Biophys Acta. 1985 Jan 9;833(1):44–51. doi: 10.1016/0005-2760(85)90251-6. [DOI] [PubMed] [Google Scholar]
  134. Pullinger C. R., North J. D., Teng B. B., Rifici V. A., Ronhild de Brito A. E., Scott J. The apolipoprotein B gene is constitutively expressed in HepG2 cells: regulation of secretion by oleic acid, albumin, and insulin, and measurement of the mRNA half-life. J Lipid Res. 1989 Jul;30(7):1065–1077. [PubMed] [Google Scholar]
  135. Quarfordt S. H., Hanks J., Shelburne F., Schirmer B. Differing uptake of emulsion triglyceride by the fed and fasted rat liver. J Clin Invest. 1982 May;69(5):1092–1098. doi: 10.1172/JCI110544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  136. Reaven G. M., Chen Y. D. Role of insulin in regulation of lipoprotein metabolism in diabetes. Diabetes Metab Rev. 1988 Nov;4(7):639–652. doi: 10.1002/dmr.5610040703. [DOI] [PubMed] [Google Scholar]
  137. Reaven G. M., Greenfield M. S. Diabetic hypertriglyceridemia: evidence for three clinical syndromes. Diabetes. 1981;30(Suppl 2):66–75. doi: 10.2337/diab.30.2.s66. [DOI] [PubMed] [Google Scholar]
  138. Risser T. R., Reaven G. M., Reaven E. P. Intestinal contribution to secretion of very low density lipoproteins into plasma. Am J Physiol. 1978 Mar;234(3):E277–E281. doi: 10.1152/ajpendo.1978.234.3.E277. [DOI] [PubMed] [Google Scholar]
  139. Risser T. R., Reaven G. M., Reaven E. P. Intestinal very low density lipoprotein secretion in insulin-deficient rats. Diabetes. 1978 Sep;27(9):902–908. doi: 10.2337/diab.27.9.902. [DOI] [PubMed] [Google Scholar]
  140. Ronne H., Ocklind C., Wiman K., Rask L., Obrink B., Peterson P. A. Ligand-dependent regulation of intracellular protein transport: effect of vitamin a on the secretion of the retinol-binding protein. J Cell Biol. 1983 Mar;96(3):907–910. doi: 10.1083/jcb.96.3.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  141. Salam W. H., Wilcox H. G., Heimberg M. Effects of oleic acid on the biosynthesis of lipoprotein apoproteins and distribution into the very-low-density lipoprotein by the isolated perfused rat liver. Biochem J. 1988 May 1;251(3):809–816. doi: 10.1042/bj2510809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  142. Schonfeld G., Pfleger B. Overproduction of very low-density lipoproteins by livers of genetically obese rats. Am J Physiol. 1971 May;220(5):1178–1181. doi: 10.1152/ajplegacy.1971.220.5.1178. [DOI] [PubMed] [Google Scholar]
  143. Schonfeld G., Pfleger B. Utilization of exogenous free fatty acids for the production of very low density lipoprotein triglyceride by livers of carbohydrate-fed rats. J Lipid Res. 1971 Sep;12(5):614–621. [PubMed] [Google Scholar]
  144. Shaheen O., Morgan D. W., Wilcox H. G., Keyes W. G., Heimberg M. Modulation by thyroid status of the actions of glucagon and dibutyryl adenosine 3',5'-monophosphate on metabolism of free fatty acids by the isolated perfused rat liver. Endocrinology. 1982 May;110(5):1740–1748. doi: 10.1210/endo-110-5-1740. [DOI] [PubMed] [Google Scholar]
  145. Siuta-Mangano P., Janero D. R., Lane M. D. Association and assembly of triglyceride and phospholipid with glycosylated and unglycosylated apoproteins of very low density lipoprotein in the intact liver cell. J Biol Chem. 1982 Oct 10;257(19):11463–11467. [PubMed] [Google Scholar]
  146. Soutar A. K. Lipoprotein-remnant receptors: second receptor verified? Nature. 1989 Sep 14;341(6238):106–107. doi: 10.1038/341106a0. [DOI] [PubMed] [Google Scholar]
  147. Sparks C. E., Marsh J. B. Metabolic heterogeneity of apolipoprotein B in the rat. J Lipid Res. 1981 Mar;22(3):519–527. [PubMed] [Google Scholar]
  148. Sparks C. E., Sparks J. D., Bolognino M., Salhanick A., Strumph P. S., Amatruda J. M. Insulin effects on apolipoprotein B lipoprotein synthesis and secretion by primary cultures of rat hepatocytes. Metabolism. 1986 Dec;35(12):1128–1136. doi: 10.1016/0026-0495(86)90026-0. [DOI] [PubMed] [Google Scholar]
  149. Sparks J. D., Sparks C. E., Bolognino M., Roncone A. M., Jackson T. K., Amatruda J. M. Effects of nonketotic streptozotocin diabetes on apolipoprotein B synthesis and secretion by primary cultures of rat hepatocytes. J Clin Invest. 1988 Jul;82(1):37–43. doi: 10.1172/JCI113597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  150. Sparks J. D., Sparks C. E., Miller L. L. Insulin effects on apolipoprotein B production by normal, diabetic and treated-diabetic rat liver and cultured rat hepatocytes. Biochem J. 1989 Jul 1;261(1):83–88. doi: 10.1042/bj2610083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  151. Sparks J. D., Sparks C. E., Roncone A. M., Amatruda J. M. Secretion of high and low molecular weight phosphorylated apolipoprotein B by hepatocytes from control and diabetic rats. Phosphorylation of APO BH and APO BL. J Biol Chem. 1988 Apr 15;263(11):5001–5004. [PubMed] [Google Scholar]
  152. Sprang S. R., Acharya K. R., Goldsmith E. J., Stuart D. I., Varvill K., Fletterick R. J., Madsen N. B., Johnson L. N. Structural changes in glycogen phosphorylase induced by phosphorylation. Nature. 1988 Nov 17;336(6196):215–221. doi: 10.1038/336215a0. [DOI] [PubMed] [Google Scholar]
  153. Stein O., Stein Y. Lipid synthesis, intracellular transport, storage, and secretion. I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats. J Cell Biol. 1967 May;33(2):319–339. doi: 10.1083/jcb.33.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  154. Storlien L. H., Kraegen E. W., Chisholm D. J., Ford G. L., Bruce D. G., Pascoe W. S. Fish oil prevents insulin resistance induced by high-fat feeding in rats. Science. 1987 Aug 21;237(4817):885–888. doi: 10.1126/science.3303333. [DOI] [PubMed] [Google Scholar]
  155. Strobl W., Gorder N. L., Fienup G. A., Lin-Lee Y. C., Gotto A. M., Jr, Patsch W. Effect of sucrose diet on apolipoprotein biosynthesis in rat liver. Increase in apolipoprotein E gene transcription. J Biol Chem. 1989 Jan 15;264(2):1190–1194. [PubMed] [Google Scholar]
  156. Struck D. K., Siuta P. B., Lane M. D., Lennarz W. J. Effect of tunicamycin on the secretion of serum proteins by primary cultures of rat and chick hepatocytes. Studies on transferrin, very low density lipoprotein, and serum albumin. J Biol Chem. 1978 Aug 10;253(15):5332–5337. [PubMed] [Google Scholar]
  157. Taskinen M. R., Nikkilä E. A., Välimäki M., Sane T., Kuusi T., Kesäniemi A., Ylikahri R. Alcohol-induced changes in serum lipoproteins and in their metabolism. Am Heart J. 1987 Feb;113(2 Pt 2):458–464. doi: 10.1016/0002-8703(87)90614-4. [DOI] [PubMed] [Google Scholar]
  158. Topping D. L., Mayes P. A. Insulin and non-esterified fatty acids. Acute regulators of lipogenesis in perfused rat liver. Biochem J. 1982 May 15;204(2):433–439. doi: 10.1042/bj2040433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  159. Topping D. L., Storer G. B., Trimble R. P. Effects of flow rate and insulin on triacylglycerol secretion by perfused rat liver. Am J Physiol. 1988 Sep;255(3 Pt 1):E306–E313. doi: 10.1152/ajpendo.1988.255.3.E306. [DOI] [PubMed] [Google Scholar]
  160. Unger R. H., Orci L. Physiology and pathophysiology of glucagon. Physiol Rev. 1976 Oct;56(4):778–826. doi: 10.1152/physrev.1976.56.4.778. [DOI] [PubMed] [Google Scholar]
  161. Van Golde L. M., Fleischer B., Fleischer S. Some studies on the metabolism of phospholipids in Golgi complex from bovine and rat liver in comparison to other subcellular fractions. Biochim Biophys Acta. 1971 Oct 12;249(1):318–330. doi: 10.1016/0005-2736(71)90109-x. [DOI] [PubMed] [Google Scholar]
  162. Vauhkonen M., Viitala J., Parkkinen J., Rauvala H. High-mannose structure of apolipoprotein-B from low-density lipoproteins of human plasma. Eur J Biochem. 1985 Oct 1;152(1):43–50. doi: 10.1111/j.1432-1033.1985.tb09161.x. [DOI] [PubMed] [Google Scholar]
  163. Venkatesan S., Ward R. J., Peters T. J. Effect of chronic ethanol feeding on the hepatic secretion of very-low-density lipoproteins. Biochim Biophys Acta. 1988 May 2;960(1):61–66. doi: 10.1016/0005-2760(88)90009-4. [DOI] [PubMed] [Google Scholar]
  164. Vogelberg K. H., Gries F. A., Moschinski D. Hepatic production of VLDL-triglycerides. Dependence of portal substrate and insulin concentration. Horm Metab Res. 1980 Dec;12(12):688–694. doi: 10.1055/s-2007-999233. [DOI] [PubMed] [Google Scholar]
  165. Wang S. R., Infante J., Catala D., Petit D., Bonnefis M. T., Infante R. Lipid and lipoprotein synthesis in isolated and cultured hepatocytes from lean and obese Zucker rats. Biochim Biophys Acta. 1989 Apr 26;1002(3):302–311. doi: 10.1016/0005-2760(89)90344-5. [DOI] [PubMed] [Google Scholar]
  166. Weiland D., Mondon C. E., Reaven G. M. Evidence for multiple causality in the development of diabetic hypertriglyceridaemia. Diabetologia. 1980 Apr;18(4):335–340. doi: 10.1007/BF00251016. [DOI] [PubMed] [Google Scholar]
  167. Wetterau J. R., Zilversmit D. B. A triglyceride and cholesteryl ester transfer protein associated with liver microsomes. J Biol Chem. 1984 Sep 10;259(17):10863–10866. [PubMed] [Google Scholar]
  168. Wilcox H. G., Heimberg M. Secretion and uptake of nascent hepatic very low density lipoprotein by perfused livers from fed and fasted rats. J Lipid Res. 1987 Apr;28(4):351–360. [PubMed] [Google Scholar]
  169. Williamson D. H. Role of insulin in the integration of lipid metabolism in mammalian tissues. Biochem Soc Trans. 1989 Feb;17(1):37–40. doi: 10.1042/bst0170037. [DOI] [PubMed] [Google Scholar]
  170. Windmueller H. G., Spaeth A. E. De novo synthesis of fatty acid in perfused rat liver as a determinant of plasma lipoprotein production. Arch Biochem Biophys. 1967 Nov;122(2):362–369. doi: 10.1016/0003-9861(67)90206-8. [DOI] [PubMed] [Google Scholar]
  171. Windmueller H. G., Spaeth A. E. Regulated biosynthesis and divergent metabolism of three forms of hepatic apolipoprotein B in the rat. J Lipid Res. 1985 Jan;26(1):70–81. [PubMed] [Google Scholar]
  172. Witztum J. L., Schonfeld G. Lipoproteins in the plasma and hepatic perfusates of the Zucker fatty rat. Diabetes. 1979 May;28(5):509–516. doi: 10.2337/diab.28.5.509. [DOI] [PubMed] [Google Scholar]
  173. Wolfe R. R., Durkot M. J. Role of very low density lipoproteins in the energy metabolism of the rat. J Lipid Res. 1985 Feb;26(2):210–217. [PubMed] [Google Scholar]
  174. Wong L., Pino R. M. Biogenesis of very-low-density lipoproteins in rat liver. Intracellular distribution of apolipoprotein B. Eur J Biochem. 1987 Apr 15;164(2):357–367. doi: 10.1111/j.1432-1033.1987.tb11066.x. [DOI] [PubMed] [Google Scholar]
  175. Wong S. H., Nestel P. J., Trimble R. P., Storer G. B., Illman R. J., Topping D. L. The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver. Biochim Biophys Acta. 1984 Feb 9;792(2):103–109. doi: 10.1016/0005-2760(84)90209-1. [DOI] [PubMed] [Google Scholar]
  176. Woodside W. F., Heimberg M. Effects of anti-insulin serum, insulin, and glucose on output of triglycerides and on ketogenesis by the perfused rat liver. J Biol Chem. 1976 Jan 10;251(1):13–23. [PubMed] [Google Scholar]
  177. Woodside W. F., Heimberg M. The metabolism of oleic acid by the perfused rat liver in experimental diabetes induced by antiinsulin serum. Metabolism. 1978 Dec;27(12):1763–1777. doi: 10.1016/0026-0495(78)90262-7. [DOI] [PubMed] [Google Scholar]
  178. Wu A. L., Windmueller H. G. Variant forms of plasma apolipoprotein B. Hepatic and intestinal biosynthesis and heterogeneous metabolism in the rat. J Biol Chem. 1981 Apr 25;256(8):3615–3618. [PubMed] [Google Scholar]
  179. Yamamoto M., Yamamoto I., Tanaka Y., Ontko J. A. Fatty acid metabolism and lipid secretion by perfused livers from rats fed laboratory stock and sucrose-rich diets. J Lipid Res. 1987 Oct;28(10):1156–1165. [PubMed] [Google Scholar]
  180. Yang C. Y., Chen S. H., Gianturco S. H., Bradley W. A., Sparrow J. T., Tanimura M., Li W. H., Sparrow D. A., DeLoof H., Rosseneu M. Sequence, structure, receptor-binding domains and internal repeats of human apolipoprotein B-100. Nature. 1986 Oct 23;323(6090):738–742. doi: 10.1038/323738a0. [DOI] [PubMed] [Google Scholar]
  181. Yao Z. M., Vance D. E. The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. J Biol Chem. 1988 Feb 25;263(6):2998–3004. [PubMed] [Google Scholar]
  182. Young S. G., Bertics S. J., Scott T. M., Dubois B. W., Curtiss L. K., Witztum J. L. Parallel expression of the MB19 genetic polymorphism in apoprotein B-100 and apoprotein B-48. Evidence that both apoproteins are products of the same gene. J Biol Chem. 1986 Mar 5;261(7):2995–2998. [PubMed] [Google Scholar]
  183. Zech L. A., Boston R. C., Foster D. M. The methodology of compartmental modeling as applied to the investigation of lipoprotein metabolism. Methods Enzymol. 1986;129:366–384. doi: 10.1016/0076-6879(86)29080-1. [DOI] [PubMed] [Google Scholar]

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