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. 1990 Jul 1;269(1):25–29. doi: 10.1042/bj2690025

Purification and characterization of a rat brain aldehyde dehydrogenase able to metabolize gamma-aminobutyraldehyde to gamma-aminobutyric acid.

T Abe 1, K Takada 1, K Ohkawa 1, M Matsuda 1
PMCID: PMC1131525  PMID: 2375753

Abstract

An enzyme which catalyses dehydrogenation of gamma-aminobutyraldehyde (ABAL) to gamma-aminobutyric acid (GABA) was purified to homogeneity from rat brain tissues by using DEAE-cellulose and affinity chromatography on 5'-AMP-Sepharose, phosphocellulose and Blue Agarose, followed by gel filtration. Such an enzyme was first purified from mammalian brain tissues, and was identified as an isoenzyme of aldehyde dehydrogenase. It has an Mr of 210,000 determined by polyacrylamide-gradient-gel electrophoresis, and appeared to be composed of subunits of Mr 50,000. The close similarity of substrate specificity toward acetaldehyde, propionaldehyde and glycolaldehyde between the enzyme and other aldehyde dehydrogenases previously reported was observed. But substrate specificity of the enzyme toward ABAL was higher than those of aldehyde dehydrogenases from human liver (E1 and E2), and was lower than those of ABAL dehydrogenases from human liver (E3), Escherichia coli and Pseudomonas species. The Mr and relative amino acid composition of the enzyme are also similar to those of E1 and E2. The existence of this enzyme in mammalian brain seems to be related to a glutamate decarboxylase-independent pathway (alternative pathway) for GABA synthesis from putrescine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambroziak W., Pietruszko R. Human aldehyde dehydrogenase: metabolism of putrescine and histamine. Alcohol Clin Exp Res. 1987 Dec;11(6):528–532. doi: 10.1111/j.1530-0277.1987.tb00167.x. [DOI] [PubMed] [Google Scholar]
  2. Asakura T., Ikeda Y., Matsuda M. Distribution of activity converting 4-aminobutyraldehyde to gamma-aminobutyric acid in subcellular fractions of mouse brain. J Neurochem. 1989 Feb;52(2):448–452. doi: 10.1111/j.1471-4159.1989.tb09141.x. [DOI] [PubMed] [Google Scholar]
  3. Callewaert D. M., Rosemblatt M. S., Tchen T. T. Purification and properties of 4-aminobutanal dehydrogenase from a Pseudomonas species. J Biol Chem. 1974 Mar 25;249(6):1737–1741. [PubMed] [Google Scholar]
  4. Cornish-Bowden A. Relating proteins by amino acid composition. Methods Enzymol. 1983;91:60–75. doi: 10.1016/s0076-6879(83)91011-x. [DOI] [PubMed] [Google Scholar]
  5. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  6. De Mello F. G., Bachrach U., Nirenberg M. Ornithine and glutamic acid decarboxylase activities in the developing chick retina. J Neurochem. 1976 Oct;27(4):847–851. doi: 10.1111/j.1471-4159.1976.tb05145.x. [DOI] [PubMed] [Google Scholar]
  7. Eckfeldt J., Mope L., Takio K., Yonetani T. Horse liver aldehyde dehydrogenase. Purification and characterization of two isozymes. J Biol Chem. 1976 Jan 10;251(1):236–240. [PubMed] [Google Scholar]
  8. Forte-McRobbie C. M., Pietruszko R. Purification and characterization of human liver "high Km" aldehyde dehydrogenase and its identification as glutamic gamma-semialdehyde dehydrogenase. J Biol Chem. 1986 Feb 15;261(5):2154–2163. [PubMed] [Google Scholar]
  9. Greenfield N. J., Pietruszko R. Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes. Biochim Biophys Acta. 1977 Jul 8;483(1):35–45. doi: 10.1016/0005-2744(77)90005-5. [DOI] [PubMed] [Google Scholar]
  10. Kitabatake N., Sasaki R., Chiba H. Localization of bovine liver aldehyde dehydrogenase isozymes and their immunological properties. J Biochem. 1981 Apr;89(4):1223–1229. [PubMed] [Google Scholar]
  11. Kremzner L. T., Hiller J. M., Simon E. J. Metabolism of polyamines in mouse neuroblastoma cells in culture: formation of GABA and putreanine. J Neurochem. 1975 Dec;25(6):889–894. doi: 10.1111/j.1471-4159.1975.tb04423.x. [DOI] [PubMed] [Google Scholar]
  12. Kurys G., Ambroziak W., Pietruszko R. Human aldehyde dehydrogenase. Purification and characterization of a third isozyme with low Km for gamma-aminobutyraldehyde. J Biol Chem. 1989 Mar 15;264(8):4715–4721. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Prieto M. I., Martin J., Balaña-Fouce R., Garrido-Pertierra A. Properties of gamma-aminobutyraldehyde dehydrogenase from Escherichia coli. Biochimie. 1987 Nov-Dec;69(11-12):1161–1168. doi: 10.1016/0300-9084(87)90142-8. [DOI] [PubMed] [Google Scholar]
  16. ROBERTS E., FRANKEL S. gamma-Aminobutyric acid in brain: its formation from glutamic acid. J Biol Chem. 1950 Nov;187(1):55–63. [PubMed] [Google Scholar]
  17. Ryzlak M. T., Pietruszko R. Purification and characterization of aldehyde dehydrogenase from human brain. Arch Biochem Biophys. 1987 Jun;255(2):409–418. doi: 10.1016/0003-9861(87)90409-7. [DOI] [PubMed] [Google Scholar]
  18. Seiler N., Al-Therib M. J. Putrescine catabolism in mammalian brain. Biochem J. 1974 Oct;144(1):29–35. doi: 10.1042/bj1440029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seiler N., al-Therib M. J., Kataoka K. Formation of GABA from putrescine in the brain of fish (Salmo irideus Gibb.). J Neurochem. 1973 Mar;20(3):699–708. doi: 10.1111/j.1471-4159.1973.tb00030.x. [DOI] [PubMed] [Google Scholar]
  20. Senior D. J., Tsai C. S. Purification and characterization of aldehyde dehydrogenase from rat liver mitochondria. Arch Biochem Biophys. 1988 Apr;262(1):211–220. doi: 10.1016/0003-9861(88)90183-x. [DOI] [PubMed] [Google Scholar]
  21. Sugahara M., Asakura T., Kurioka S., Matsuda M. 4-Aminobutyraldehyde as a substance convertible in vivo to GABA. J Neurochem. 1983 Jan;40(1):294–296. doi: 10.1111/j.1471-4159.1983.tb12686.x. [DOI] [PubMed] [Google Scholar]
  22. Tago K., Kurioka S., Matsuda M. 4-Aminobutyraldehyde dehydrogenase activity in rat brain. J Neurochem. 1982 Sep;39(3):803–809. doi: 10.1111/j.1471-4159.1982.tb07963.x. [DOI] [PubMed] [Google Scholar]

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