Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Aug 15;270(1):103–107. doi: 10.1042/bj2700103

Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.

A Gorchein 1
PMCID: PMC1131684  PMID: 2396971

Abstract

A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.

Full text

PDF
103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beale S. I., Castelfranco P. A. The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: II. Formation of C-delta-Aminolevulinic Acid from Labeled Precursors in Greening Plant Tissues. Plant Physiol. 1974 Feb;53(2):297–303. doi: 10.1104/pp.53.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. F., Devey K. R., McBride L., Desnick R. J. Rapid determination of delta-aminolevulinate synthase activity by a specific fluorometric coupled enzyme assay. Anal Biochem. 1981 May 1;113(1):68–78. doi: 10.1016/0003-2697(81)90045-2. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Brodie M. J., Thompson G. G., Moore M. R., Beattie A. D., Goldberg A. Hereditary coproporphyria. Demonstration of the abnormalities in haem biosynthesis in peripheral blood. Q J Med. 1977 Apr;46(182):229–241. [PubMed] [Google Scholar]
  5. Brooker J. D., Srivastava G., May B. K., Elliott W. H. Radiochemical assay for gamma-aminolevulinate synthase. Enzyme. 1982;28(2-3):109–119. doi: 10.1159/000459095. [DOI] [PubMed] [Google Scholar]
  6. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  7. De Matteis F., Ray D. E. Studied on cerebellar haem metabolism in the rat in vivo. J Neurochem. 1982 Aug;39(2):551–556. doi: 10.1111/j.1471-4159.1982.tb03980.x. [DOI] [PubMed] [Google Scholar]
  8. Fitzsimons E. J., May A., Elder G. H., Jacobs A. Measurement of 5-aminolevulinic acid synthase activity in whole and fractionated human bone marrow: effect of myeloid cell lysis by monoclonal antibody. Anal Biochem. 1986 Feb 15;153(1):9–17. doi: 10.1016/0003-2697(86)90053-9. [DOI] [PubMed] [Google Scholar]
  9. Freshney R. I., Paul J. Measurement of aminolaevulinate synthetase activity in normal mouse liver with [2-14C] glycine. Biochim Biophys Acta. 1970 Dec 16;220(3):594–601. doi: 10.1016/0005-2744(70)90289-5. [DOI] [PubMed] [Google Scholar]
  10. Gorchein A. Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection. Biochem J. 1984 May 1;219(3):883–889. doi: 10.1042/bj2190883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorchein A., Webber R. delta-Aminolaevulinic acid in plasma, cerebrospinal fluid, saliva and erythrocytes: studies in normal, uraemic and porphyric subjects. Clin Sci (Lond) 1987 Jan;72(1):103–112. doi: 10.1042/cs0720103. [DOI] [PubMed] [Google Scholar]
  12. Lien L. F., Beattie D. S. Comparisons and modifications of the colorimetric assay for delta-aminolevulinic acid synthase. Enzyme. 1982;28(2-3):120–132. doi: 10.1159/000459096. [DOI] [PubMed] [Google Scholar]
  13. MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
  14. Percy V. A., Shanley B. C. Studies on haem biosynthesis in rat brain. J Neurochem. 1979 Dec;33(6):1267–1274. doi: 10.1111/j.1471-4159.1979.tb05273.x. [DOI] [PubMed] [Google Scholar]
  15. Strand L. J., Swanson A. L., Manning J., Branch S., Marver H. S. Radiochemical microassay of delta-aminolevulinic acid synthetase in hepatic and erythroid tissues. Anal Biochem. 1972 Jun;47(2):457–470. doi: 10.1016/0003-2697(72)90139-x. [DOI] [PubMed] [Google Scholar]
  16. Tikerpae J., Samson D., Lim C. K. Determination of delta-aminolaevulinic acid synthase activity in human bone marrow using high performance liquid chromatography. Clin Chim Acta. 1981 Jun 2;113(1):65–73. doi: 10.1016/0009-8981(81)90441-1. [DOI] [PubMed] [Google Scholar]
  17. URATA G., GRANICK S. Biosynthesis of alpha-aminoketones and the metabolism of aminoacetone. J Biol Chem. 1963 Feb;238:811–820. [PubMed] [Google Scholar]
  18. Yoda B., Schacter B. A., Israels L. G. Delta-aminolevulinic acid synthetase assay in chicken liver homogenates and particulate fractions. Anal Biochem. 1975 May 26;66(1):221–233. doi: 10.1016/0003-2697(75)90740-x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES