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. 1990 Aug 15;270(1):175–180. doi: 10.1042/bj2700175

Intermediates of peroxisomal beta-oxidation. A study of the fatty acyl-CoA esters which accumulate during peroxisomal beta-oxidation of [U-14C]hexadecanoate.

K Bartlett 1, R Hovik 1, S Eaton 1, N J Watmough 1, H Osmundsen 1
PMCID: PMC1131695  PMID: 2396977

Abstract

1. 14C-labelled fatty acyl-CoA esters resulting from beta-oxidation of [U-14C]hexadecanoate by peroxisomal fractions isolated from rats treated with clofibrate showed the presence of the full range of saturated intermediates down to acetyl-CoA. 2. The pattern of intermediates generated was fairly constant. At low concentrations of [U-14C]hexadecanoate (50 microM), decanoyl-CoA was present in lowest amounts. At higher concentrations of [U-14C]hexadecanoate (greater than 100 microM), all intermediates of chain length shorter than 12 carbon atoms (except acetyl-CoA) were present at similar low concentrations; the process of beta-oxidation now resembling chain-shortening of hexadecanoate by two cycles of beta-oxidation. 3. In the absence of an NAD(+)-regenerating system [pyruvate and lactate dehydrogenase (EC 1.1.1.28)] 2-enoyl- and 3-hydroxyacyl-CoA esters were generated, suggesting that re-oxidation of NADH is essential for optimal rates of peroxisomal beta-oxidation in vitro. 4. At high concentrations of [U-14C]hexadecanoate (greater than 100 microM), 3-oxohexadecanoyl-CoA was produced, suggesting that thiolase (acetyl-CoA acetyltransferase; EC 2.3.1.9) can become rate-limiting for peroxisomal beta-oxidation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexson S. E., Osmundsen H., Berge R. K. The presence of acyl-CoA hydrolase in rat brown-adipose-tissue peroxisomes. Biochem J. 1989 Aug 15;262(1):41–46. doi: 10.1042/bj2620041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Christiansen E. N., Thomassen M. S., Christiansen R. Z., Osmundsen H., Norum K. R. Metabolism of erucic acid in perfused rat liver: increased chain shortening after feeding partially hydrogenated marine oil and rapeseed oil. Lipids. 1979 Oct;14(10):829–835. doi: 10.1007/BF02534124. [DOI] [PubMed] [Google Scholar]
  3. Christiansen R. Z. The effect of clofibrate-feeding on hepatic fatty acid metabolism. Biochim Biophys Acta. 1978 Sep 28;530(3):314–324. doi: 10.1016/0005-2760(78)90151-0. [DOI] [PubMed] [Google Scholar]
  4. Hiltunen J. K., Kärki T., Hassinen I. E., Osmundsen H. beta-Oxidation of polyunsaturated fatty acids by rat liver peroxisomes. A role for 2,4-dienoyl-coenzyme A reductase in peroxisomal beta-oxidation. J Biol Chem. 1986 Dec 15;261(35):16484–16493. [PubMed] [Google Scholar]
  5. Hovik R., Osmundsen H. Peroxisomal beta-oxidation of long-chain fatty acids possessing different extents of unsaturation. Biochem J. 1987 Nov 1;247(3):531–535. doi: 10.1042/bj2470531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lazarow P. B., De Duve C. A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2043–2046. doi: 10.1073/pnas.73.6.2043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lazarow P. B. Rat liver peroxisomes catalyze the beta oxidation of fatty acids. J Biol Chem. 1978 Mar 10;253(5):1522–1528. [PubMed] [Google Scholar]
  8. Leighton F., Bergseth S., Rørtveit T., Christiansen E. N., Bremer J. Free acetate production by rat hepatocytes during peroxisomal fatty acid and dicarboxylic acid oxidation. J Biol Chem. 1989 Jun 25;264(18):10347–10350. [PubMed] [Google Scholar]
  9. Neat C. E., Thomassen M. S., Osmundsen H. Effects of high-fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient. Biochem J. 1981 Apr 15;196(1):149–159. doi: 10.1042/bj1960149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Osmundsen H. Factors which can influence beta-oxidation by peroxisomes isolated from livers of clofibrate treated rats. Some properties of peroxisomal fractions isolated in a self-generated Percoll gradient by vertical rotor centrifugation. Int J Biochem. 1982;14(10):905–914. doi: 10.1016/0020-711x(82)90074-x. [DOI] [PubMed] [Google Scholar]
  11. Osmundsen H., Neat C. E., Norum K. R. Peroxisomal oxidation of long chain fatty acids. FEBS Lett. 1979 Mar 15;99(2):292–296. doi: 10.1016/0014-5793(79)80975-8. [DOI] [PubMed] [Google Scholar]
  12. Watmough N. J., Bhuiyan A. K., Bartlett K., Sherratt H. S., Turnbull D. M. Skeletal muscle mitochondrial beta-oxidation. A study of the products of oxidation of [U-14C]hexadecanoate by h.p.l.c. using continuous on-line radiochemical detection. Biochem J. 1988 Jul 15;253(2):541–547. doi: 10.1042/bj2530541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Watmough N. J., Turnbull D. M., Sherratt H. S., Bartlett K. Measurement of the acyl-CoA intermediates of beta-oxidation by h.p.l.c. with on-line radiochemical and photodiode-array detection. Application to the study of [U-14C]hexadecanoate oxidation by intact rat liver mitochondria. Biochem J. 1989 Aug 15;262(1):261–269. doi: 10.1042/bj2620261. [DOI] [PMC free article] [PubMed] [Google Scholar]

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