Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Aug 15;270(1):261–263. doi: 10.1042/bj2700261

Alternative splicing of glucokinase mRNA in rat liver.

D J Hayzer 1, P B Iynedjian 1
PMCID: PMC1131708  PMID: 2396986

Abstract

The sequences of two near full-length cDNAs encoding rat liver glucokinase are reported. One of the cDNAs is essentially identical to the cDNA cloned by Andreone, Printz, Pilkis, Magnuson & Granner. [(1989) J. Biol. Chem. 264, 363-369]. The other cDNA contains a 151 bp insertion and a downstream 52 bp deletion. The inserted block of bases has been shown to originate from an optional cassette exon, termed 2A, between the previously described exons 1 and 2. The conceptual translation product from the variant mRNA is identical to the original glucokinase protein for the first 15 amino acids. Next there is a novel polypeptide sequence of 87 residues, comprising 50 residues encoded by the cassette exon and 37 residues specified by an altered reading frame in exon 2. Due to the 52 bp deletion, 17 amino acids of the reference sequence are then missing, after which the sequence reverts to the original. Northern blot analysis with oligonucleotide probes has shown that alternatively spliced mRNA represents about 5% of total glucokinase mRNA. Alternative splicing of glucokinase mRNA in liver may explain earlier findings of minor isoforms of hepatic glucokinase.

Full text

PDF
261

Images in this article

263Fig. 3.
on p.263

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. B., Walker D. G. Multiple forms of hepatic glucokinase. Biochem Soc Trans. 1976;4(6):1057–1057. doi: 10.1042/bst0041057. [DOI] [PubMed] [Google Scholar]
  2. Andreone T. L., Printz R. L., Pilkis S. J., Magnuson M. A., Granner D. K. The amino acid sequence of rat liver glucokinase deduced from cloned cDNA. J Biol Chem. 1989 Jan 5;264(1):363–369. [PubMed] [Google Scholar]
  3. Dale R. M., McClure B. A., Houchins J. P. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid. 1985 Jan;13(1):31–40. doi: 10.1016/0147-619x(85)90053-8. [DOI] [PubMed] [Google Scholar]
  4. Iynedjian P. B., Möbius G., Seitz H. J., Wollheim C. B., Renold A. E. Tissue-specific expression of glucokinase: identification of the gene product in liver and pancreatic islets. Proc Natl Acad Sci U S A. 1986 Apr;83(7):1998–2001. doi: 10.1073/pnas.83.7.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Iynedjian P. B., Pilot P. R., Nouspikel T., Milburn J. L., Quaade C., Hughes S., Ucla C., Newgard C. B. Differential expression and regulation of the glucokinase gene in liver and islets of Langerhans. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7838–7842. doi: 10.1073/pnas.86.20.7838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iynedjian P. B., Ucla C., Mach B. Molecular cloning of glucokinase cDNA. Developmental and dietary regulation of glucokinase mRNA in rat liver. J Biol Chem. 1987 May 5;262(13):6032–6038. [PubMed] [Google Scholar]
  7. Magnuson M. A., Andreone T. L., Printz R. L., Koch S., Granner D. K. Rat glucokinase gene: structure and regulation by insulin. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4838–4842. doi: 10.1073/pnas.86.13.4838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Magnuson M. A., Shelton K. D. An alternate promoter in the glucokinase gene is active in the pancreatic beta cell. J Biol Chem. 1989 Sep 25;264(27):15936–15942. [PubMed] [Google Scholar]
  9. Meglasson M. D., Burch P. T., Berner D. K., Najafi H., Vogin A. P., Matschinsky F. M. Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans. Proc Natl Acad Sci U S A. 1983 Jan;80(1):85–89. doi: 10.1073/pnas.80.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newgard C. B., Quaade C., Hughes S. D., Milburn J. L. Glucokinase and glucose transporter expression in liver and islets: implications for control of glucose homoeostasis. Biochem Soc Trans. 1990 Oct;18(5):851–853. doi: 10.1042/bst0180851. [DOI] [PubMed] [Google Scholar]
  11. Smith C. W., Patton J. G., Nadal-Ginard B. Alternative splicing in the control of gene expression. Annu Rev Genet. 1989;23:527–577. doi: 10.1146/annurev.ge.23.120189.002523. [DOI] [PubMed] [Google Scholar]
  12. Weinhouse S. Regulation of glucokinase in liver. Curr Top Cell Regul. 1976;11:1–50. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES