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. 1990 Aug 15;270(1):273–276. doi: 10.1042/bj2700273

Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells.

S Ullrich 1, M Prentki 1, C B Wollheim 1
PMCID: PMC1131711  PMID: 1975739

Abstract

Somatostatin inhibited Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells, albeit with decreased sensitivity relative to intact cells. The inhibitory action required the presence of GTP, whereas GDP could not substitute for GTP. Pertussis-toxin treatment before cell permeabilization abolished the inhibition of secretion. Thus somatostatin, by activating a G-protein, interferes with exocytosis distal to the generation of soluble intracellular messengers.

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Selected References

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