Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Aug 15;270(1):273–276. doi: 10.1042/bj2700273

Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells.

S Ullrich 1, M Prentki 1, C B Wollheim 1
PMCID: PMC1131711  PMID: 1975739

Abstract

Somatostatin inhibited Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells, albeit with decreased sensitivity relative to intact cells. The inhibitory action required the presence of GTP, whereas GDP could not substitute for GTP. Pertussis-toxin treatment before cell permeabilization abolished the inhibition of secretion. Thus somatostatin, by activating a G-protein, interferes with exocytosis distal to the generation of soluble intracellular messengers.

Full text

PDF
273

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amiranoff B., Lorinet A. M., Lagny-Pourmir I., Laburthe M. Mechanism of galanin-inhibited insulin release. Occurrence of a pertussis-toxin-sensitive inhibition of adenylate cyclase. Eur J Biochem. 1988 Oct 15;177(1):147–152. doi: 10.1111/j.1432-1033.1988.tb14355.x. [DOI] [PubMed] [Google Scholar]
  2. Barber D. L., McGuire M. E., Ganz M. B. Beta-adrenergic and somatostatin receptors regulate Na-H exchange independent of cAMP. J Biol Chem. 1989 Dec 15;264(35):21038–21042. [PubMed] [Google Scholar]
  3. Basabe J. C., Cresto J. C., Aparicio N. Studies on the mode of action of somatostatin on insulin secretion. Endocrinology. 1977 Nov;101(5):1436–1443. doi: 10.1210/endo-101-5-1436. [DOI] [PubMed] [Google Scholar]
  4. Breckenridge L. J., Almers W. Currents through the fusion pore that forms during exocytosis of a secretory vesicle. 1987 Aug 27-Sep 2Nature. 328(6133):814–817. doi: 10.1038/328814a0. [DOI] [PubMed] [Google Scholar]
  5. Dunne M. J., Bullett M. J., Li G. D., Wollheim C. B., Petersen O. H. Galanin activates nucleotide-dependent K+ channels in insulin-secreting cells via a pertussis toxin-sensitive G-protein. EMBO J. 1989 Feb;8(2):413–420. doi: 10.1002/j.1460-2075.1989.tb03392.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hierowski M. T., Liebow C., du Sapin K., Schally A. V. Stimulation by somatostatin of dephosphorylation of membrane proteins in pancreatic cancer MIA PaCa-2 cell line. FEBS Lett. 1985 Jan 7;179(2):252–256. doi: 10.1016/0014-5793(85)80529-9. [DOI] [PubMed] [Google Scholar]
  7. Hughes S. J., Ashcroft S. J. Effect of secretagogues on cytosolic free Ca2+ and insulin release in the hamster clonal beta-cell line HIT-T15. J Mol Endocrinol. 1988 Jul;1(1):13–17. doi: 10.1677/jme.0.0010013. [DOI] [PubMed] [Google Scholar]
  8. Jones P. M., Fyles J. M., Persaud S. J., Howell S. L. Catecholamine inhibition of Ca2+-induced insulin secretion from electrically permeabilised islets of Langerhans. FEBS Lett. 1987 Jul 13;219(1):139–144. doi: 10.1016/0014-5793(87)81206-1. [DOI] [PubMed] [Google Scholar]
  9. Jones P. M., Salmon D. M., Howell S. L. Protein phosphorylation in electrically permeabilized islets of Langerhans. Effects of Ca2+, cyclic AMP, a phorbol ester and noradrenaline. Biochem J. 1988 Sep 1;254(2):397–403. doi: 10.1042/bj2540397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Katada T., Ui M. Islet-activating protein. Enhanced insulin secretion and cyclic AMP accumulation in pancreatic islets due to activation of native calcium ionophores. J Biol Chem. 1979 Jan 25;254(2):469–479. [PubMed] [Google Scholar]
  11. Koch B. D., Dorflinger L. J., Schonbrunn A. Pertussis toxin blocks both cyclic AMP-mediated and cyclic AMP-independent actions of somatostatin. Evidence for coupling of Ni to decreases in intracellular free calcium. J Biol Chem. 1985 Oct 25;260(24):13138–13145. [PubMed] [Google Scholar]
  12. Lewis D. L., Weight F. F., Luini A. A guanine nucleotide-binding protein mediates the inhibition of voltage-dependent calcium current by somatostatin in a pituitary cell line. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9035–9039. doi: 10.1073/pnas.83.23.9035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liebow C., Reilly C., Serrano M., Schally A. V. Somatostatin analogues inhibit growth of pancreatic cancer by stimulating tyrosine phosphatase. Proc Natl Acad Sci U S A. 1989 Mar;86(6):2003–2007. doi: 10.1073/pnas.86.6.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Luini A., De Matteis M. A. Evidence that receptor-linked G protein inhibits exocytosis by a post-second-messenger mechanism in AtT-20 cells. J Neurochem. 1990 Jan;54(1):30–38. doi: 10.1111/j.1471-4159.1990.tb13279.x. [DOI] [PubMed] [Google Scholar]
  15. Meglasson M. D., Manning C. D., Najafi H., Matschinsky F. M. Fuel-stimulated insulin secretion by clonal hamster beta-cell line HIT T-15. Diabetes. 1987 Apr;36(4):477–484. doi: 10.2337/diab.36.4.477. [DOI] [PubMed] [Google Scholar]
  16. Nilsson T., Arkhammar P., Rorsman P., Berggren P. O. Suppression of insulin release by galanin and somatostatin is mediated by a G-protein. An effect involving repolarization and reduction in cytoplasmic free Ca2+ concentration. J Biol Chem. 1989 Jan 15;264(2):973–980. [PubMed] [Google Scholar]
  17. Pace C. S., Murphy M., Conant S., Lacy P. E. Somatostatin inhibition of glucose-induced electrical activity in cultured rat islet cells. Am J Physiol. 1977 Nov;233(5):C165–C171. doi: 10.1152/ajpcell.1977.233.5.C164. [DOI] [PubMed] [Google Scholar]
  18. Prentki M., Janjic D., Wollheim C. B. The regulation of extramitochondrial steady state free Ca2+ concentration by rat insulinoma mitochondria. J Biol Chem. 1983 Jun 25;258(12):7597–7602. [PubMed] [Google Scholar]
  19. Reichlin S. Somatostatin (second of two parts). N Engl J Med. 1983 Dec 22;309(25):1556–1563. doi: 10.1056/NEJM198312223092506. [DOI] [PubMed] [Google Scholar]
  20. Reichlin S. Somatostatin. N Engl J Med. 1983 Dec 15;309(24):1495–1501. doi: 10.1056/NEJM198312153092406. [DOI] [PubMed] [Google Scholar]
  21. Rostene W. H., Dussaillant M., Rosselin G. Rapid inhibition by somatostatin of vasoactive intestinal peptide-induced prolactin secretion by rat pituitary cells. Relationship to cyclic AMP accumulation. FEBS Lett. 1982 Sep 6;146(1):213–216. doi: 10.1016/0014-5793(82)80738-2. [DOI] [PubMed] [Google Scholar]
  22. Santerre R. F., Cook R. A., Crisel R. M., Sharp J. D., Schmidt R. J., Williams D. C., Wilson C. P. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4339–4343. doi: 10.1073/pnas.78.7.4339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schlegel W., Wuarin F., Zbaren C., Wollheim C. B., Zahnd G. R. Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH3 cells. FEBS Lett. 1985 Sep 9;189(1):27–32. doi: 10.1016/0014-5793(85)80835-8. [DOI] [PubMed] [Google Scholar]
  24. Sweatt J. D., Volterra A., Edmonds B., Karl K. A., Siegelbaum S. A., Kandel E. R. FMRFamide reverses protein phosphorylation produced by 5-HT and cAMP in Aplysia sensory neurons. Nature. 1989 Nov 16;342(6247):275–278. doi: 10.1038/342275a0. [DOI] [PubMed] [Google Scholar]
  25. Swope S. L., Schonbrunn A. Bombesin stimulates insulin secretion by a pancreatic islet cell line. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1822–1826. doi: 10.1073/pnas.81.6.1822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tamagawa T., Niki H., Niki A. Insulin release independent of a rise in cytosolic free Ca2+ by forskolin and phorbol ester. FEBS Lett. 1985 Apr 22;183(2):430–432. doi: 10.1016/0014-5793(85)80825-5. [DOI] [PubMed] [Google Scholar]
  27. Ullrich S., Wollheim C. B. GTP-dependent inhibition of insulin secretion by epinephrine in permeabilized RINm5F cells. Lack of correlation between insulin secretion and cyclic AMP levels. J Biol Chem. 1988 Jun 25;263(18):8615–8620. [PubMed] [Google Scholar]
  28. Ullrich S., Wollheim C. B. Galanin inhibits insulin secretion by direct interference with exocytosis. FEBS Lett. 1989 Apr 24;247(2):401–404. doi: 10.1016/0014-5793(89)81379-1. [DOI] [PubMed] [Google Scholar]
  29. Wollheim C. B., Blondel B., Kikuchi M., Sharp G. W. Inhibition of insulin release by somatostatin: no evidence for interaction with calcium. Metabolism. 1978 Sep;27(9 Suppl 1):1303–1307. doi: 10.1016/0026-0495(78)90063-x. [DOI] [PubMed] [Google Scholar]
  30. Wollheim C. B., Kikuchi M., Renold A. E., Sharp G. W. Somatostatin- and epinephrine-induced modifications of 45Ca++ fluxes and insulin release in rat pancreatic islets maintained in tissue culture. J Clin Invest. 1977 Nov;60(5):1165–1173. doi: 10.1172/JCI108869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yamashita N., Shibuya N., Ogata E. Requirement of GTP on somatostatin-induced K+ current in human pituitary tumor cells. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4924–4928. doi: 10.1073/pnas.85.13.4924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. de Weille J. R., Schmid-Antomarchi H., Fosset M., Lazdunski M. Regulation of ATP-sensitive K+ channels in insulinoma cells: activation by somatostatin and protein kinase C and the role of cAMP. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2971–2975. doi: 10.1073/pnas.86.8.2971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES