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. 1992 Dec 15;288(Pt 3):941–943. doi: 10.1042/bj2880941

Calf chymosin as a catalyst of peptide synthesis.

C A Abdel Malak 1
PMCID: PMC1131977  PMID: 1472007

Abstract

Calf chymosin was shown to catalyse peptide synthesis optimally over the range pH 4-5, giving satisfactory yields of methyl esters or p-nitroanilides of benzyloxycarbonyl tetra- to hexa-peptides, provided that hydrophobic amino-acid residues form the new peptide bonds. The effectiveness of the enzyme depends also on the nature of adjacent amino-acid residues. As an aspartate-proteinase with a characteristic specificity pattern chymosin would be useful for the synthesis of middle-length peptides.

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Selected References

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