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. 1993 Mar 1;290(Pt 2):591–594. doi: 10.1042/bj2900591

Snake venom cardiotoxin can rapidly induce actin polymerization in intact platelets.

R F Liou 1, W C Chang 1, S T Chu 1, Y H Chen 1
PMCID: PMC1132315  PMID: 8452550

Abstract

The action of Taiwan cobra (Naja naja atra) venom cardiotoxin on rabbit platelets at 37 degrees C was characterized by observing cytoskeletal alterations and cell lysis. At a concentration of 21.4 microM the toxin produced no cell lysis within 30 s, and less than 5% of the total lactate dehydrogenase activity of intact cells was detected in the suspending medium after the interaction had proceeded for 3 min. The extent of cell lysis was proportional to toxin concentration and interaction time. Before cell lysis, the toxin caused rapid incorporation of actin monomers into cross-linked actin filaments. The actin incorporation could be inhibited by either the presence of cytochalasin B or increased CaCl2 concentration in the suspending medium. However, addition of indomethacin did not influence the toxin-induced cytoskeletal change.

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Selected References

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