Abstract
Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction.
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- Axelson M., Mörk B., Sjövall J. Occurrence of 3 beta-hydroxy-5-cholestenoic acid, 3 beta,7 alpha-dihydroxy-5-cholestenoic acid, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acid as normal constituents in human blood. J Lipid Res. 1988 May;29(5):629–641. [PubMed] [Google Scholar]
- Bandi Z. L., Reynolds E. S. Adsorption and reversed-phase high-performance liquid chromatography of p-nitrobenzyl esters of monohydroxy fatty acids. J Chromatogr. 1985 Jul 5;329(1):57–63. doi: 10.1016/s0021-9673(01)81895-1. [DOI] [PubMed] [Google Scholar]
- Christie W. W. Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection. J Lipid Res. 1985 Apr;26(4):507–512. [PubMed] [Google Scholar]
- Kaluzny M. A., Duncan L. A., Merritt M. V., Epps D. E. Rapid separation of lipid classes in high yield and purity using bonded phase columns. J Lipid Res. 1985 Jan;26(1):135–140. [PubMed] [Google Scholar]
- McGrath L. T., Elliott R. J. Lipid analysis and fatty acid profiles of individual arterial atherosclerotic plaques. Anal Biochem. 1990 Jun;187(2):273–276. doi: 10.1016/0003-2697(90)90456-j. [DOI] [PubMed] [Google Scholar]
- Sotirhos N., Herslöf B., Kenne L. Quantitative analysis of phospholipids by 31P-NMR. J Lipid Res. 1986 Apr;27(4):386–392. [PubMed] [Google Scholar]
- Sze D. Y., Jardetzky O. Characterization of lipid composition in stimulated human lymphocytes by 1H-NMR. Biochim Biophys Acta. 1990 Sep 1;1054(2):198–206. doi: 10.1016/0167-4889(90)90241-5. [DOI] [PubMed] [Google Scholar]