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. 1993 Apr 1;291(Pt 1):19–21. doi: 10.1042/bj2910019

A new, highly sensitive method for the detection and quantification of penicillin-binding proteins.

M Galleni 1, B Lakaye 1, S Lepage 1, M Jamin 1, I Thamm 1, B Joris 1, J M Frère 1
PMCID: PMC1132474  PMID: 8385929

Abstract

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

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Selected References

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