Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1993 Apr 15;291(Pt 2):345–347. doi: 10.1042/bj2910345

Activation of oxidized cysteine proteinases by thioredoxin-mediated reduction in vitro.

A G Stephen 1, R Powls 1, R J Beynon 1
PMCID: PMC1132530  PMID: 8484715

Abstract

Activity of the cysteine adducts of the cysteine proteinases papain and thaumatopain can be recovered by treatment with thioredoxin, thioredoxin reductase and NADPH. Recovery of proteinase activity did not occur if any of the components of the thioredoxin system were omitted, or if thioredoxin or thioredoxin reductase were heat-inactivated. Such an enzyme-mediated process may be of significance in the recovery of cysteine proteinases inactivated by oxidative attack.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett A. J., Kembhavi A. A., Brown M. A., Kirschke H., Knight C. G., Tamai M., Hanada K. L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J. 1982 Jan 1;201(1):189–198. doi: 10.1042/bj2010189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumgartner B., Chrispeels M. J. Purification and characterization of vicilin peptidohydrolase, the major endopeptidase in the cotyledons of mung-bean seedlings. Eur J Biochem. 1977 Jul 15;77(2):223–233. doi: 10.1111/j.1432-1033.1977.tb11661.x. [DOI] [PubMed] [Google Scholar]
  3. Cusack M., Stephen A. G., Powls R., Beynon R. J. Purification and characterization of thaumatopain, a cysteine protease from the arils of the plant Thaumatococcus daniellii. Biochem J. 1991 Feb 15;274(Pt 1):231–236. doi: 10.1042/bj2740231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FERDINAND W., STEIN W. H., MOORE S. AN UNUSUAL DISULFIDE BOND IN STREPTOCOCCAL PROTEINASE. J Biol Chem. 1965 Mar;240:1150–1155. [PubMed] [Google Scholar]
  5. Gorpinchenko T. V., Vakar A. B., Kretovich V. L. Issledozanie Proteindisul'fidreduktazy Pshchenitsy. Biokhimiia. 1975 Mar-Apr;40(2):323–330. [PubMed] [Google Scholar]
  6. HATCH M. D., TURNER J. F. A protein disulphide reductase from pea seeds. Biochem J. 1960 Sep;76:556–562. doi: 10.1042/bj0760556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmgren A. Thioredoxin and glutaredoxin systems. J Biol Chem. 1989 Aug 25;264(24):13963–13966. [PubMed] [Google Scholar]
  8. Holmgren A. Thioredoxin. Annu Rev Biochem. 1985;54:237–271. doi: 10.1146/annurev.bi.54.070185.001321. [DOI] [PubMed] [Google Scholar]
  9. KIMMEL J. R., SMITH E. L. Crystalline papain. I. Preparation, specificity, and activation. J Biol Chem. 1954 Apr;207(2):515–531. [PubMed] [Google Scholar]
  10. Klein I. B., Kirsch J. F. The mechanism of the activation of papain. Biochem Biophys Res Commun. 1969 Mar 10;34(5):575–581. doi: 10.1016/0006-291x(69)90776-1. [DOI] [PubMed] [Google Scholar]
  11. Kobrehel K., Wong J. H., Balogh A., Kiss F., Yee B. C., Buchanan B. B. Specific reduction of wheat storage proteins by thioredoxin h. Plant Physiol. 1992 Jul;99(3):919–924. doi: 10.1104/pp.99.3.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koehler S. M., Ho T. H. Hormonal regulation, processing, and secretion of cysteine proteinases in barley aleurone layers. Plant Cell. 1990 Aug;2(8):769–783. doi: 10.1105/tpc.2.8.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koehler S., Ho T. H. Purification and characterization of gibberellic Acid-induced cysteine endoproteases in barley aleurone layers. Plant Physiol. 1988 May;87(1):95–103. doi: 10.1104/pp.87.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lo S. S., Fraser B. A., Liu T. Y. The mixed disulfide in the zymogen of streptococcal proteinase. Characterization and implication for its biosynthesis. J Biol Chem. 1984 Sep 10;259(17):11041–11045. [PubMed] [Google Scholar]
  15. Sluyterman L. A. The activation reaction of papain. Biochim Biophys Acta. 1967 Jul 11;139(2):430–438. doi: 10.1016/0005-2744(67)90046-0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES